In cellulo and in vivo assays for compound testing against Trypanosoma cruzi.

Humans Trypanosoma cruzi Chagas Disease / drug therapy
Cell Biology Cell-based Assays High-throughput Screening Microbiology Model Organisms

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
17 03 2023
Historique:
received: 16 08 2022
revised: 18 09 2022
accepted: 03 01 2023
medline: 23 10 2023
pubmed: 1 3 2023
entrez: 28 2 2023
Statut: ppublish

Résumé

Here, we describe a combined in cellulo and in vivo approach to identify compounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC

Identifiants

DOI: 10.1016/j.xpro.2023.102058 PMID: 36853683 PMC: PMC9881407
pubmed: 36853683
pii: S2666-1667(23)00016-3
doi: 10.1016/j.xpro.2023.102058
pmc: PMC9881407
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102058

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

Auteurs

Eloise Pavão Guerra-Slompo (EP)

Instituto Carlos Chagas, Fiocruz Paraná, Curitiba, Paraná 81350-010, Brazil. Electronic address: eloise.slompo@fiocruz.br.

Gisele Fernanda Assine Picchi-Constante (GFA)

Instituto Carlos Chagas, Fiocruz Paraná, Curitiba, Paraná 81350-010, Brazil. Electronic address: gisele.picchi@fiocruz.br.

Martin Marek (M)

Université de Strasbourg, CNRS, INSERM, Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, U 1258, 67404 Illkirch, France; IGBMC, Department of Integrated Structural Biology, 1 rue Laurent Fries, B.P. 10142, 67404 Illkirch Cedex, France.

Christophe Romier (C)

Université de Strasbourg, CNRS, INSERM, Institut de Génétique et de Biologie Moléculaire et Cellulaire, UMR 7104, U 1258, 67404 Illkirch, France; IGBMC, Department of Integrated Structural Biology, 1 rue Laurent Fries, B.P. 10142, 67404 Illkirch Cedex, France.

Wolfgang Sippl (W)

Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, Wolfgang-Langenbeck-Straße 4, 06120 Halle/Saale, Germany.

Nilson Ivo Tonin Zanchin (NIT)

Instituto Carlos Chagas, Fiocruz Paraná, Curitiba, Paraná 81350-010, Brazil. Electronic address: nilson.zanchin@fiocruz.br.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › In cellulo and in vivo assays for compound...
questionsmedicales.fr › Eucaryotes › Euglénozoaires › Kinetoplastida › Trypanosomatina › Trypanosoma › Trypanosoma cruzi › In cellulo and in vivo assays for compound...
questionsmedicales.fr › Infection › Maladies vectorielles › Maladie de Chagas › In cellulo and in vivo assays for compound...