Intranasal administration of interleukin-4 ameliorates depression-like behavior and biochemical alterations in mouse submitted to the chronic unpredictable mild stress: modulation of neuroinflammation and oxidative stress.


Journal

Psychopharmacology
ISSN: 1432-2072
Titre abrégé: Psychopharmacology (Berl)
Pays: Germany
ID NLM: 7608025

Informations de publication

Date de publication:
Apr 2023
Historique:
received: 30 03 2022
accepted: 03 02 2023
pubmed: 2 3 2023
medline: 15 3 2023
entrez: 1 3 2023
Statut: ppublish

Résumé

Physical and psychological stress modulates the hypothalamic pituitary adrenal (HPA) axis, and the redox and inflammatory systems. Impairments in these systems have been extensively reported in major depression (MD) patients. Therefore, our study aimed to investigate the effects of the intranasal administration of interleukin-4 (IL-4) in mice with depressive-like behavior induced by chronic unpredictable mild stress (CUMS) for 28 days. On the 28th day, mice received IL-4 intranasally (1 ng/mouse) or vehicle (sterile saline), and after 30 min, they were submitted to behavioral tests or euthanasia for blood collection and removal of the adrenal glands, axillary lymph nodes, spleen, thymus, prefrontal cortices (PFC), and hippocampi (HC). A single administration of IL-4 reversed CUMS-induced depression-like behavior in the tail suspension test and splash test, without evoking locomotor changes. IL-4 administration reduced the plasma levels of corticosterone and the increased weight of suprarenal glands in stressed mice. Moreover, IL-4 restored the expression of nuclear factor erythroid 2-related factor 2 (NRF2), nuclear factor kappa B (NF-kB), interleukin 1 beta (IL-1β), IL-4, brain derived neurotrophic factor (BDNF), and indoleamine 2,3-dioxygenase (IDO) in the PFC and HC and modulated oxidative stress markers in these brain structures in stressed mice. Our results showed for the first time the antidepressant-like effect of IL-4 through the modulation of neuroinflammation and oxidative stress. The potential effect of IL-4 administered intranasally arises as an innovative strategy for MD treatment.

Identifiants

pubmed: 36856802
doi: 10.1007/s00213-023-06336-4
pii: 10.1007/s00213-023-06336-4
doi:

Substances chimiques

Interleukin-4 207137-56-2
Brain-Derived Neurotrophic Factor 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

935-950

Informations de copyright

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

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Auteurs

Thiago Ângelo Smaniotto (TÂ)

Technology Development Center, Division of Biotechnology, Neurobiotechnology Research Group, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Angela Maria Casaril (AM)

Technology Development Center, Division of Biotechnology, Neurobiotechnology Research Group, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Darling de Andrade Lourenço (D)

Technology Development Center, Division of Biotechnology, Neurobiotechnology Research Group, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Fernanda S Sousa (FS)

Technology Development Center, Division of Biotechnology, Cancer Biotechnology Laboratory, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Fabiana K Seixas (FK)

Technology Development Center, Division of Biotechnology, Cancer Biotechnology Laboratory, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Tiago Collares (T)

Technology Development Center, Division of Biotechnology, Cancer Biotechnology Laboratory, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Rafael Woloski (R)

Technology Development Center, Division of Biotechnology, Bioinformatics and Proteomics Laboratory, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Luciano da Silva Pinto (L)

Technology Development Center, Division of Biotechnology, Bioinformatics and Proteomics Laboratory, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Diego Alves (D)

Center of Chemical, Pharmaceutical and Food Sciences, Laboratory of Clean Organic Synthesis, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil.

Lucielli Savegnago (L)

Technology Development Center, Division of Biotechnology, Neurobiotechnology Research Group, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil. luciellisavegnago@yahoo.com.br.
Center of Chemical, Pharmaceutical and Food Sciences, Laboratory of Clean Organic Synthesis, Federal University of Pelotas, Pelotas, RS, 96010900, Brazil. luciellisavegnago@yahoo.com.br.

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