Effectiveness of conservative instrumentation in root canal disinfection.
Conservative instrumentation
Disinfection
Polymicrobial infection
Root canal preparation
Journal
Clinical oral investigations
ISSN: 1436-3771
Titre abrégé: Clin Oral Investig
Pays: Germany
ID NLM: 9707115
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
17
10
2022
accepted:
22
02
2023
medline:
15
6
2023
pubmed:
4
3
2023
entrez:
3
3
2023
Statut:
ppublish
Résumé
The impact of conservative instrumentation on the disinfection of root canals with different curvatures has not yet been determined. This ex vivo study aimed to evaluate and compare the effect of conservative instrumentation with TruNatomy (TN) and Rotate and a conventional rotary system, ProTaper Gold (PTG), on root canal disinfection during chemomechanical preparation of straight and curved canals. Ninety mandibular molars with straight (n = 45) and curved (n = 45) mesiobuccal root canals were contaminated with polymicrobial clinical samples. Teeth were divided into three subgroups (n = 14) according to the file systems and the curvature. Canals were instrumented with TN, Rotate, and PTG, respectively. Sodium hypochlorite and EDTA were used as irrigants. Intracanal samples were taken before (S1) and after (S2) instrumentation. Six uninfected teeth were used as negative controls. The bacterial reduction between S1 and S2 was measured by ATP assay, flow cytometry, and culture methods. Kruskal-Wallis and ANOVA tests were followed by the Duncan post hoc test (p < 0.05). Bacterial reduction percentages were similar for the three file systems in straight canals (p > 0.05). However, PTG showed a lower reduction percentage of intact membrane cells in flow cytometry than TN and Rotate (p = 0.036). For the curved canals, no significant differences were obtained (p > 0.05). Conservative instrumentation of straight and curved canals using TN and Rotate files resulted in similar bacterial reduction compared to PTG. The disinfection efficacy of conservative instrumentation is similar to conventional instrumentation in straight and curved root canals.
Identifiants
pubmed: 36867258
doi: 10.1007/s00784-023-04929-z
pii: 10.1007/s00784-023-04929-z
pmc: PMC10264279
doi:
Substances chimiques
Sodium Hypochlorite
DY38VHM5OD
Root Canal Irrigants
0
Types de publication
Journal Article
Langues
eng
Pagination
3181-3188Informations de copyright
© 2023. The Author(s).
Références
Siqueira JF Jr, Rôças IN (2022) Present status and future directions: microbiology of endodontic infections. Int Endod J 55(Suppl 3):512–530. https://doi.org/10.1111/iej.13677
doi: 10.1111/iej.13677
pubmed: 34958494
Hall-Stoodley L, Costerton JW, Stoodley P (2004) Bacterial biofilms: from the natural environment to infectious diseases. Nat Rev Microbiol 2:95–108. https://doi.org/10.1038/nrmicro821
doi: 10.1038/nrmicro821
pubmed: 15040259
Haapasalo M, Endal U, Zandi H, Coil JM (2005) Eradication of endodontic infection by instrumentation and irrigation solutions. Endod Topics 10:77–102. https://doi.org/10.1111/j.1601-1546.2005.00135.x
doi: 10.1111/j.1601-1546.2005.00135.x
Xu J, Gao Y, Meng Y, Wu W, Tsauo C, Guo T et al (2020) Mechano-chemical coupling of irrigation enhances endodontic biofilm debridement. Biofouling 36:792–799. https://doi.org/10.1080/08927014.2020.1814753
doi: 10.1080/08927014.2020.1814753
pubmed: 32873073
Castelo-Baz P, Lozano FJR, Ginzo-Villamayor MJ, Vila RM, Seoane-Romero J, Martín-Cruces J et al (2021) Efficacy of continuous apical negative ultrasonic irrigation (CANUI) in penetration of simulated lateral canals in extracted teeth. Sci Rep 11:10908. https://doi.org/10.1038/s41598-021-90430-0
doi: 10.1038/s41598-021-90430-0
pubmed: 34035414
pmcid: 8149429
Metzger Z, Solomonov M, Kfir A (2013) The role of mechanical instrumentation in the cleaning of root canals. Endod Topics 29:87–109. https://doi.org/10.1111/etp.12048
doi: 10.1111/etp.12048
Ruiz-Linares M, Aguado-Pérez B, Baca P, Arias-Moliz M, Ferrer-Luque C (2017) Efficacy of antimicrobial solutions against polymicrobial root canal biofilm. Int Endod J 50:77–83. https://doi.org/10.1111/iej.12598
doi: 10.1111/iej.12598
pubmed: 26660494
Gluskin AH, Peters CI, Peters OA (2014) Minimally invasive endodontics: challenging prevailing paradigms. Br Dent J 216:347–353. https://doi.org/10.1038/sj.bdj.2014.201
doi: 10.1038/sj.bdj.2014.201
pubmed: 24651341
Plotino G, Özyürek T, Grande NM, Gündoğar M (2019) Influence of size and taper of basic root canal preparation on root canal cleanliness: a scanning electron microscopy study. Int Endod J 52:343–351. https://doi.org/10.1111/iej.13002
doi: 10.1111/iej.13002
pubmed: 30129186
Perez Morales MLN, González Sánchez JA, OlivieriFernández JG, Laperre K, AbellaSans F, Jaramillo DE et al (2020) TRUShape versus XP-endo shaper: a micro-computed tomographic assessment and comparative study of the shaping ability-an in vitro study. J Endod 46:271–276. https://doi.org/10.1016/j.joen.2019.10.027
doi: 10.1016/j.joen.2019.10.027
pubmed: 31839412
Roane JB, Sabala CL, Duncanson MG Jr (1985) The “balanced force” concept for instrumentation of curved canals. J Endod 11:203–211. https://doi.org/10.1016/s0099-2399(85)80061-3
doi: 10.1016/s0099-2399(85)80061-3
pubmed: 3858415
DentsplySirona. TruNatomy Brochure. Available from: https://assets.dentsplysirona.com/flagship/en/explore/endodontics/brochure/trunatomy/END-TruNatomy-Brochure.pdf . Accessed 8 Dec 2021
VDW. WDV.Rotate Brochure [Available from: https://www.vdw-dental.com/fileadmin/Dokumente/Sortiment/Aufbereitung/Rotierende-Aufbereitung/ROTATE/VDW-Dental-ROTATE-Step-by-Step-Card-EN.pdf . Accessed 8 Dec 2021
Boutsioukis C, Gutierrez Nova P (2021) Syringe irrigation in minimally shaped root canals using 3 endodontic needles: a computational fluid dynamics study. J Endod 47:1487–1495. https://doi.org/10.1016/j.joen.2021.06.001
doi: 10.1016/j.joen.2021.06.001
pubmed: 34118256
Silva EJNL, de Lima CO, Barbosa AFA, Lopes RT, Sassone LM, Versiani MA (2022) The impact of TruNatomy and ProTaper Gold instruments on the preservation of the periradicular dentin and on the enlargement of the apical canal of mandibular molars. J Endod 48:650–658. https://doi.org/10.1016/j.joen.2022.02.003
doi: 10.1016/j.joen.2022.02.003
pubmed: 35181453
Gündoğar M, Uslu G, Özyürek T, Plotino G (2020) Comparison of the cyclic fatigue resistance of VDW ROTATE, TruNatomy, 2Shape, and HyFlex CM nickel-titanium rotary files at body temperature. Restor Dent Endod 45:37. https://doi.org/10.5395/rde.2020.45.e37
doi: 10.5395/rde.2020.45.e37
Al Omari T, El-Farraj H, Arıcan B, AtavAteş A (2021) Apical debris extrusion of full-sequenced rotary systems in narrow ribbon-shaped canals. Aust Endod J 48:245–250. https://doi.org/10.1111/aej.12540
doi: 10.1111/aej.12540
pubmed: 34125465
Martinho FC, Gomes AP, Fernandes AM, Ferreira NS, Endo MS, Freitas LF et al (2014) Clinical comparison of the effectiveness of single-file reciprocating systems and rotary systems for removal of endotoxins and cultivable bacteria from primarily infected root canals. J Endod 40:625–629. https://doi.org/10.1016/j.joen.2013.12.006
doi: 10.1016/j.joen.2013.12.006
pubmed: 24767554
Schneider SW (1971) A comparison of canal preparations in straight and curved root canals. Oral Surg Oral Med Oral Pathol 32:271–275. https://doi.org/10.1016/0030-4220(71)90230-1
doi: 10.1016/0030-4220(71)90230-1
pubmed: 5284110
Wang L, Lin R, Chen H, Li Z, Tay FR, Gu L (2022) Influence of the number of pecking motions at working length on the shaping ability of single-file systems in long oval-shaped curved canals. J Endod 48:548–554. https://doi.org/10.1016/j.joen.2022.01.009
doi: 10.1016/j.joen.2022.01.009
pubmed: 35081440
Nakamura VC, Kataoka SH, Gavini G, Ferrari PH, Cai S (2012) FE-SEM evaluation of dental specimens prepared by different methods for in vitro contamination. Int J Dent 2012:748471. https://doi.org/10.1155/2012/748471
doi: 10.1155/2012/748471
pubmed: 22505908
pmcid: 3312258
Rôças IN, Siqueira JF Jr (2011) In vivo antimicrobial effects of endodontic treatment procedures as assessed by molecular microbiologic techniques. J Endod 37:304–310. https://doi.org/10.1016/j.joen.2010.11.003
doi: 10.1016/j.joen.2010.11.003
pubmed: 21329812
Cohen J (1988) Statistical power analysis for the behavioral sciences. Hillsdale, N.J.: L. Erlbaum Associates
Farrugia C, Lung CYK, SchembriWismayer P, Arias-Moliz MT, Camilleri J (2018) The relationship of surface characteristics and antimicrobial performance of pulp capping materials. J Endod 44:1115–1120. https://doi.org/10.1016/j.joen.2018.04.002
doi: 10.1016/j.joen.2018.04.002
pubmed: 29861063
Pérez AR, Alves FRF, Marceliano-Alves MF, Provenzano JC, Gonçalves LS, Neves AA et al (2018) Effects of increased apical enlargement on the amount of unprepared areas and coronal dentine removal: a micro-computed tomography study. Int Endod J 51:684–690. https://doi.org/10.1111/iej.12873
doi: 10.1111/iej.12873
pubmed: 29112281
Rodrigues RCV, Zandi H, Kristoffersen AK, Enersen M, Mdala I, Ørstavik D et al (2017) Influence of the apical preparation size and the irrigant type on bacterial reduction in root canal-treated teeth with apical periodontitis. J Endod 43:1058–1063. https://doi.org/10.1016/j.joen.2017.02.004
doi: 10.1016/j.joen.2017.02.004
pubmed: 28483164
Boutsioukis C, Arias-Moliz MT, Chávez de Paz LE (2022) A critical analysis of research methods and experimental models to study irrigants and irrigation systems. Int Endod J 55:295–329. https://doi.org/10.1111/iej.13710
doi: 10.1111/iej.13710
pubmed: 35171506
pmcid: 9314845
Camilleri J, Arias Moliz T, Bettencourt A, Costa J, Martins F, Rabadijeva D et al (2020) Standardization of antimicrobial testing of dental devices. Dent Mater 36:e59–e73. https://doi.org/10.1016/j.dental.2019.12.006
doi: 10.1016/j.dental.2019.12.006
pubmed: 31928776
Sánchez MC, Llama-Palacios A, Marín MJ, Figuero E, León R, Blanc V et al (2013) Validation of ATP bioluminescence as a tool to assess antimicrobial effects of mouthrinses in an in vitro subgingival-biofilm model. Med Oral Patol Oral Cir Bucal 18:e86-92. https://doi.org/10.4317/medoral.18376
doi: 10.4317/medoral.18376
pubmed: 23229259
Netuschil L, Auschill TM, Sculean A, Arweiler NB (2014) Confusion over live/dead stainings for the detection of vital microorganisms in oral biofilms–which stain is suitable? BMC Oral Health 14:2. https://doi.org/10.1186/1472-6831-14-2
doi: 10.1186/1472-6831-14-2
pubmed: 24410850
pmcid: 3898065
Ahmed HMA (2022) A critical analysis of laboratory and clinical research methods to study root and canal anatomy. Int Endod J 55(Suppl 2):229–280. https://doi.org/10.1111/iej.13702
doi: 10.1111/iej.13702
pubmed: 35124829
Zhang X, Xiong S, Ma Y, Han T, Chen X, Wan F et al (2015) A cone-beam computed tomographic study on mandibular first molars in a Chinese subpopulation. PLoS One 10:e0134919. https://doi.org/10.1371/journal.pone.0134919
doi: 10.1371/journal.pone.0134919
pubmed: 26241480
pmcid: 4524594
Saber SE, Schäfer E (2016) Incidence of dentinal defects after preparation of severely curved root canals using the Reciproc single-file system with and without prior creation of a glide path. Int Endod J 49:1057–1064. https://doi.org/10.1111/iej.12555
doi: 10.1111/iej.12555
pubmed: 26426069
Petričević GK, Katić M, Anić I, Salarić I, Vražić D, Bago I (2022) Efficacy of different Er:YAG laser-activated photoacoustic streaming modes compared to passive ultrasonic irrigation in the retreatment of curved root canals. Clin Oral Investig 26:6773–6781. https://doi.org/10.1007/s00784-022-04637-0
doi: 10.1007/s00784-022-04637-0
pubmed: 35879623
Berutti E, Alovisi M, Moccia E, Carossa M, De Caro G, Roccuzzo A et al (2022) Micro-computed tomographic evaluation of endodontic ledge position in relation to canal curvatures. BMC Oral Health 22:482. https://doi.org/10.1186/s12903-022-02531-5
doi: 10.1186/s12903-022-02531-5
pubmed: 36357901
pmcid: 9650826
Moon W, Chung SH, Chang J (2022) Sonic irrigation for removal of calcium hydroxide in the apical root canal: a micro-CT and light-coupled tracking analysis. PLoS One 17:e0268791. https://doi.org/10.1371/journal.pone.0268791
doi: 10.1371/journal.pone.0268791
pubmed: 35653310
pmcid: 9162323
Domark JD, Hatton JF, Benison RP, Hildebolt CF (2013) An ex vivo comparison of digital radiography and cone-beam and micro computed tomography in the detection of the number of canals in the mesiobuccal roots of maxillary molars. J Endod 39:901–905. https://doi.org/10.1016/j.joen.2013.01.010
doi: 10.1016/j.joen.2013.01.010
pubmed: 23791260
pmcid: 3904227
Rhodes JS, Ford TR, Lynch JA, Liepins PJ, Curtis RV (1999) Micro-computed tomography: a new tool for experimental endodontology. Int Endod J 32:165–170. https://doi.org/10.1046/j.1365-2591.1999.00204.x
doi: 10.1046/j.1365-2591.1999.00204.x
pubmed: 10530203
Alimadadi H, Asnaashari M, Naseri M, Yadegari Z (2021) Effect of apical size and taper on the efficacy of root canal disinfection with LED photodynamic therapy as an adjunct to irrigation with sodium hypochlorite. J Lasers Med Sci 12:e58. https://doi.org/10.34172/jlms.2021.58
doi: 10.34172/jlms.2021.58
pubmed: 35155143
pmcid: 8837831
Paraskevopoulou MT, Khabbaz MG (2016) Influence of taper of root canal shape on the intracanal bacterial reduction. Open Dent J 10:568–574. https://doi.org/10.2174/1874210601610010568
doi: 10.2174/1874210601610010568
pubmed: 27857818
pmcid: 5093865
Truchado P, Gil MI, Larrosa M, Allende A (2020) Detection and quantification methods for viable but non-culturable (VBNC) cells in process wash water of fresh-cut produce: industrial validation. Front Microbiol 11:673. https://doi.org/10.3389/fmicb.2020.00673
doi: 10.3389/fmicb.2020.00673
pubmed: 32431672
pmcid: 7214806
Solana C, Ruiz-Linares M, Baca P, Valderrama MJ, Arias-Moliz MT, Ferrer-Luque CM (2017) Antibiofilm activity of sodium hypochlorite and alkaline tetrasodium EDTA solutions. J Endod 43:2093–2096. https://doi.org/10.1016/j.joen.2017.06.038
doi: 10.1016/j.joen.2017.06.038
pubmed: 28958677
Boutsioukis C, Lambrianidis T, Verhaagen B, Versluis M, Kastrinakis E, Wesselink PR et al (2010) The effect of needle-insertion depth on the irrigant flow in the root canal: evaluation using an unsteady computational fluid dynamics model. J Endod 36:1664–1668. https://doi.org/10.1016/j.joen.2010.06.023
doi: 10.1016/j.joen.2010.06.023
pubmed: 20850673
Boutsioukis C, Arias-Moliz MT (2022) Present status and future directions - irrigants and irrigation methods. Int Endod J 55:588–612. https://doi.org/10.1111/iej.13739
doi: 10.1111/iej.13739
pubmed: 35338652
pmcid: 9321999
Robberecht L, Dehurtevent M, Lemaitre G, Béhal H, Hornez JC, Claisse-Crinquette A (2017) Influence of root canal curvature on wall cleanliness in the apical third during canal preparation. Eur Endod J 2:1–6. https://doi.org/10.14744/eej.2017.16035
doi: 10.14744/eej.2017.16035
pubmed: 33403324
pmcid: 7757941
Sathorn C, Parashos P, Messer HH (2007) How useful is root canal culturing in predicting treatment outcome? J Endod 33:220–225. https://doi.org/10.1016/j.joen.2006.11.006
doi: 10.1016/j.joen.2006.11.006
pubmed: 17320700