Inadequate detection of the FSHR complicates future research on extragonadal FSHR localization.
antibodies
control
extragonadal cells
follicle stimulating hormone receptor
immunohistochemistry
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2023
2023
Historique:
received:
10
11
2022
accepted:
30
01
2023
entrez:
6
3
2023
pubmed:
7
3
2023
medline:
8
3
2023
Statut:
epublish
Résumé
Recently, follicle stimulating hormone (FSH) through interaction with its receptor (FSHR) has been proposed to play a role in postmenopausal osteoporosis and cardiovascular disease, rather than the loss of estrogen. To explore this hypothesis, unravelling which cells express extragonadal FSHR on protein level is key. We used two commercial anti-FSHR antibodies and validated them by performing immunohistochemistry on positive (ovary, testis) and negative controls (skin). The monoclonal anti-FSHR antibody could not identify the FSHR in ovary or testis. The polyclonal anti-FSHR antibody stained the granulosa cells (ovary) and Sertoli cells (testis), yet there was equally intense staining of other cells/extracellular matrix. Furthermore, the polyclonal anti-FSHR antibody also stained skin tissue extensively, suggesting that the antibody stains more than just FSHR. The findings in this study may add accuracy to literature on extragonadal FSHR localization and warrants attention to the use of inadequate anti-FSHR antibodies to value the potential role of FSH/FSHR in postmenopausal disease.
Identifiants
pubmed: 36875462
doi: 10.3389/fendo.2023.1095031
pmc: PMC9978812
doi:
Substances chimiques
Antibodies
0
Estrogens
0
Follicle Stimulating Hormone, Human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1095031Informations de copyright
Copyright © 2023 Tedjawirja, Hooijer, Savci-Heijink, Kovac, Balm and de Waard.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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