A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
07 03 2023
Historique:
received: 11 08 2022
accepted: 27 02 2023
entrez: 7 3 2023
pubmed: 8 3 2023
medline: 10 3 2023
Statut: epublish

Résumé

Adequate pain management is essential for ethical and scientific reasons in animal experiments and should completely cover the period of expected pain without the need for frequent re-application. However, current depot formulations of Buprenorphine are only available in the USA and have limited duration of action. Recently, a new microparticulate Buprenorphine formulation (BUP-Depot) for sustained release has been developed as a potential future alternative to standard formulations available in Europe. Pharmacokinetics indicate a possible effectiveness for about 72 h. Here, we investigated whether the administration of the BUP-Depot ensures continuous and sufficient analgesia in two mouse fracture models (femoral osteotomy) and could, therefore, serve as a potent alternative to the application of Tramadol via the drinking water. Both protocols were examined for analgesic effectiveness, side effects on experimental readout, and effects on fracture healing outcomes in male and female C57BL/6N mice. The BUP-Depot provided effective analgesia for 72 h, comparable to the effectiveness of Tramadol in the drinking water. Fracture healing outcome was not different between analgesic regimes. The availability of a Buprenorphine depot formulation for rodents in Europe would be a beneficial addition for extended pain relief in mice, thereby increasing animal welfare.

Identifiants

pubmed: 36882427
doi: 10.1038/s41598-023-30641-9
pii: 10.1038/s41598-023-30641-9
pmc: PMC9992384
doi:

Substances chimiques

Buprenorphine 40D3SCR4GZ
Tramadol 39J1LGJ30J
Drinking Water 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3824

Informations de copyright

© 2023. The Author(s).

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Auteurs

Angelique Wolter (A)

Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany. a.wolter@fu-berlin.de.
German Rheumatism Research Centre (DRFZ), Leibniz Institute, Berlin, Germany. a.wolter@fu-berlin.de.
Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany. a.wolter@fu-berlin.de.

Christian H Bucher (CH)

Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Berlin Institute of Health Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Berlin, Germany.

Sebastian Kurmies (S)

Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
German Rheumatism Research Centre (DRFZ), Leibniz Institute, Berlin, Germany.

Viktoria Schreiner (V)

Division of Pharmaceutical Technology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland.

Frank Konietschke (F)

Berlin Institute of Health Center for Regenerative Therapies (BCRT), Charité-Universitätsmedizin Berlin, Berlin, Germany.
Institute of Biometry and Clinical Epidemiology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.

Katharina Hohlbaum (K)

Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
German Centre for the Protection of Laboratory Animals (Bf3R), German Federal Institute for Risk Assessment (BfR), Berlin, Germany.

Robert Klopfleisch (R)

Institute of Veterinary Pathology, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.

Max Löhning (M)

Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
German Rheumatism Research Centre (DRFZ), Leibniz Institute, Berlin, Germany.

Christa Thöne-Reineke (C)

Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.

Frank Buttgereit (F)

Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
German Rheumatism Research Centre (DRFZ), Leibniz Institute, Berlin, Germany.

Jörg Huwyler (J)

Division of Pharmaceutical Technology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland.

Paulin Jirkof (P)

Office for Animal Welfare and 3Rs, University of Zurich, Zurich, Switzerland.

Anna E Rapp (AE)

Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
German Rheumatism Research Centre (DRFZ), Leibniz Institute, Berlin, Germany.
Dr. Rolf M. Schwiete Research Unit for Osteoarthritis, Department of Orthopedics (Friedrichsheim), University Hospital Frankfurt, Goethe University, Frankfurt, Germany.

Annemarie Lang (A)

Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany. Annemarie.lang@pennmedicine.upenn.edu.
German Rheumatism Research Centre (DRFZ), Leibniz Institute, Berlin, Germany. Annemarie.lang@pennmedicine.upenn.edu.
Departments of Orthopaedic Surgery and Bioengineering, University of Pennsylvania, Philadelphia, PA, USA. Annemarie.lang@pennmedicine.upenn.edu.

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Classifications MeSH