Episodic live imaging of cone photoreceptor maturation in GNAT2-EGFP retinal organoids.

CRISPR GNAT2 cone photoreceptor fluorescent reporter iPSC live imaging retinal organoid

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
01 Mar 2023
Historique:
pubmed: 14 3 2023
medline: 14 3 2023
entrez: 13 3 2023
Statut: epublish

Résumé

Fluorescent reporter pluripotent stem cell (PSC) derived retinal organoids are powerful tools to investigate cell type-specific development and disease phenotypes. When combined with live imaging, they enable direct and repeated observation of cell behaviors within a developing retinal tissue. Here, we generated a human cone photoreceptor reporter line by CRISPR/Cas9 genome editing of WTC11-mTagRFPT-LMNB1 human induced pluripotent stem cells (iPSCs) by inserting enhanced green fluorescent protein (EGFP) coding sequences and a 2A self-cleaving peptide at the N-terminus of

Identifiants

DOI: 10.1101/2023.02.28.530518 PMID: 36909527 PMC: PMC10002746
pubmed: 36909527
doi: 10.1101/2023.02.28.530518
pmc: PMC10002746
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NCI NIH HHS
ID : R01 CA137124
Pays : United States

Commentaires et corrections

Type : UpdateIn

Déclaration de conflit d'intérêts

COMPETING INTERESTS The authors declare no competing or financial interests.

Auteurs

Jinlun Bai (J)

The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA.
The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Development, Stem Cell, and Regenerative Medicine Program, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

David S Koos (DS)

The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Translational Biomedical Imaging Laboratory, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Department of Radiology, Children's Hospital Los Angeles, Los Angeles, CA, USA.

Kayla Stepanian (K)

The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA.
The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.

Zachary Fouladian (Z)

The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA.
The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Development, Stem Cell, and Regenerative Medicine Program, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Dominic W H Shayler (DWH)

The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA.
The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Development, Stem Cell, and Regenerative Medicine Program, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Jennifer G Aparicio (JG)

The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA.
The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.

Scott E Fraser (SE)

The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Translational Biomedical Imaging Laboratory, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.
Translational Imaging Center, University of Southern California, Los Angeles, CA, USA.

Rex A Moats (RA)

The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Translational Biomedical Imaging Laboratory, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Department of Radiology, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, Los Angeles, CA, USA.

David Cobrinik (D)

The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA.
The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA.
Department of Ophthalmology and Roski Eye Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Department of Biochemistry & Molecular Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Classifications MeSH