Characterization of Shigella flexneri Serotype 6 Strains Isolated from Bangladesh and Identification of a New Phylogenetic Cluster.
Bangladesh
E1037
S. flexneri 6a
S. flexneri 6b
Shigella
Shigella flexneri 6
phylogeny
shigellosis
Journal
Journal of bacteriology
ISSN: 1098-5530
Titre abrégé: J Bacteriol
Pays: United States
ID NLM: 2985120R
Informations de publication
Date de publication:
25 04 2023
25 04 2023
Historique:
medline:
27
4
2023
pubmed:
18
3
2023
entrez:
17
3
2023
Statut:
ppublish
Résumé
A significant cause of shigellosis in Bangladesh and other developing countries is Shigella flexneri serotype 6. This serotype has been subtyped, on the basis of the absence or presence of a group-specific antigen, E1037, into S. flexneri 6a and 6b, respectively. Here, we provided rationales for the subclassification, using several phenotypic and molecular tools. A set of S. flexneri 6a and 6b strains isolated between 1997 and 2015 were characterized by analyzing their biochemical properties, plasmid profiles, virulence markers, pulsed-field gel electrophoresis (PFGE) results, and ribotype. Additionally, the genomic relatedness of these subserotypes was investigated with global isolates of serotype 6 using publicly available genomes. Both subserotypes of S. flexneri 6 agglutinated with monoclonal antiserum against S. flexneri (MASF) B and type VI-specific antiserum (MASF VI) and were PCR positive for O-antigen flippase-specific genes and virulence markers (
Identifiants
pubmed: 36927058
doi: 10.1128/jb.00406-22
pmc: PMC10127597
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0040622Références
J Clin Microbiol. 2003 Jan;41(1):110-7
pubmed: 12517835
Nucleic Acids Res. 2016 Jul 8;44(W1):W242-5
pubmed: 27095192
Proc Natl Acad Sci U S A. 2000 Sep 12;97(19):10567-72
pubmed: 10954745
Microb Genom. 2021 Dec;7(12):
pubmed: 34904947
Int J Mol Sci. 2021 Nov 10;22(22):
pubmed: 34830042
J Clin Microbiol. 2002 Jul;40(7):2490-7
pubmed: 12089268
PLoS One. 2018 Aug 24;13(8):e0202704
pubmed: 30142163
Clin Lab. 2021 Apr 1;67(4):
pubmed: 33865251
Clin Infect Dis. 2021 May 18;72(10):1793-1798
pubmed: 32239137
Vaccines (Basel). 2020 Apr 03;8(2):
pubmed: 32260067
Vet World. 2022 Feb;15(2):281-287
pubmed: 35400957
Pathog Dis. 2016 Jul;74(5):
pubmed: 27106253
Biosci Rep. 2019 Sep 24;39(9):
pubmed: 31519769
Lancet Infect Dis. 2018 Nov;18(11):1229-1240
pubmed: 30266330
Braz J Microbiol. 2020 Dec;51(4):1563-1571
pubmed: 32710175
Sci Rep. 2020 Apr 30;10(1):7345
pubmed: 32355292
Elife. 2015 Aug 04;4:e07335
pubmed: 26238191
PLoS One. 2011;6(11):e27754
pubmed: 22110755
Epidemiol Infect. 1989 Jun;102(3):421-8
pubmed: 2661251
J Clin Microbiol. 2019 Mar 28;57(4):
pubmed: 30700507
Nat Commun. 2022 Jan 27;13(1):551
pubmed: 35087053
Lancet. 1975 Jan 11;1(7898):88-90
pubmed: 46035
PLoS One. 2012;7(9):e46095
pubmed: 23049947
mSphere. 2022 Jun 29;7(3):e0102021
pubmed: 35611657
Appl Microbiol Biotechnol. 2021 Feb;105(3):1159-1173
pubmed: 33452891
mSphere. 2020 Oct 7;5(5):
pubmed: 33028681
Genome Res. 2020 Jan;30(1):138-152
pubmed: 31809257
Gut Pathog. 2014 Mar 31;6(1):7
pubmed: 24684748
Lett Appl Microbiol. 2020 Jul;71(1):86-93
pubmed: 31829434
Gut Pathog. 2015 Jun 04;7:14
pubmed: 26042182
J Med Microbiol. 1987 Jun;23(4):289-302
pubmed: 2438412
J Clin Microbiol. 2001 Oct;39(10):3757-9
pubmed: 11574611
Gut Pathog. 2019 Jun 14;11:33
pubmed: 31249630
Infect Immun. 1999 Feb;67(2):782-8
pubmed: 9916090
J Clin Microbiol. 2019 Mar 28;57(4):
pubmed: 30700505
Vaccines (Basel). 2022 Mar 07;10(3):
pubmed: 35335035
Front Microbiol. 2018 Oct 31;9:2609
pubmed: 30429838
Commun Biol. 2021 Mar 19;4(1):353
pubmed: 33742111
Genome Res. 2004 Jul;14(7):1394-403
pubmed: 15231754
Foodborne Pathog Dis. 2012 May;9(5):418-24
pubmed: 22506731
Front Microbiol. 2021 Apr 12;12:628622
pubmed: 33912145
Sci Rep. 2021 Jun 17;11(1):12738
pubmed: 34140589
Curr Microbiol. 2022 Feb 27;79(4):118
pubmed: 35220467
J Bacteriol. 1995 Jun;177(11):3355-7
pubmed: 7768842
Mol Biol Evol. 2021 Apr 13;38(4):1570-1579
pubmed: 33313861
Gut Pathog. 2022 Feb 22;14(1):10
pubmed: 35193669
PLoS Negl Trop Dis. 2021 Oct 13;15(10):e0009826
pubmed: 34644291
AIMS Microbiol. 2019 Aug 13;5(3):205-222
pubmed: 31663057