Growth hormone-releasing hormone antagonists protect against hydrochloric acid-induced endothelial injury in vitro.
Endothelium
Inflammation
Lung injury
Vasculature
Journal
Environmental toxicology and pharmacology
ISSN: 1872-7077
Titre abrégé: Environ Toxicol Pharmacol
Pays: Netherlands
ID NLM: 9612020
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
received:
18
02
2023
revised:
13
03
2023
accepted:
16
03
2023
pmc-release:
01
04
2024
medline:
18
4
2023
pubmed:
21
3
2023
entrez:
20
3
2023
Statut:
ppublish
Résumé
Growth hormone-releasing hormone (GHRH) regulates the synthesis of growth hormone from the anterior pituitary gland, and it is involved in inflammatory responses. On the other hand, GHRH antagonists (GHRHAnt) exhibit the opposite effects, resulting in endothelial barrier enhancement. Exposure to hydrochloric acid (HCL) is associated with acute and chronic lung injury. In this study, we investigate the effects of GHRHAnt in HCL-induced endothelial barrier dysfunction, utilizing commercially available bovine pulmonary artery endothelial cells (BPAEC). Cell viability was measured by utilizing 3-(4,5-dimethylthiazol2-yl)- 2,5-diphenyltetrazolium bromide (MTT) assay. Moreover, fluorescein isothiocyanate (FITC)-dextran was used to assess barrier function. Our observations suggest that GHRHAnt exert protective effects against HCL-induced endothelial breakdown, since those peptides counteract HCL-triggered paracellular hyperpermeability. Based on those findings, we propose that GHRHAnt represent a new therapeutic approach towards HCL-induced endothelial injury.
Identifiants
pubmed: 36940786
pii: S1382-6689(23)00055-8
doi: 10.1016/j.etap.2023.104113
pmc: PMC10111240
mid: NIHMS1885997
pii:
doi:
Substances chimiques
Hydrochloric Acid
QTT17582CB
Growth Hormone-Releasing Hormone
9034-39-3
Growth Hormone
9002-72-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
104113Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103424
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Références
Pharmaceuticals (Basel). 2022 Jan 24;15(2):
pubmed: 35215247
Chest. 1988 Sep;94(3):476-81
pubmed: 2842114
Trends Endocrinol Metab. 2021 Jun;32(6):338-340
pubmed: 33771415
Am J Respir Cell Mol Biol. 2014 Jan;50(1):170-9
pubmed: 23972231
DNA Repair (Amst). 2020 Nov;95:102952
pubmed: 32846356
Molecules. 2022 Apr 21;27(9):
pubmed: 35566020
J Cell Mol Med. 2018 Mar;22(3):1792-1804
pubmed: 29363851
Cell Mol Life Sci. 2020 Nov;77(22):4725-4727
pubmed: 32886127
Am J Physiol Lung Cell Mol Physiol. 2015 Apr 15;308(8):L776-87
pubmed: 25713322
Inflammation. 2020 Apr;43(2):686-691
pubmed: 31838664
Autops Case Rep. 2021 Apr 20;11:e2021266
pubmed: 33968833
Br J Cancer. 2008 Jun 3;98(11):1790-6
pubmed: 18506184
Injury. 2022 Jul;53(7):2400-2412
pubmed: 35577600
Proc Natl Acad Sci U S A. 2014 Dec 23;111(51):18303-8
pubmed: 25489106
Cells. 2021 Jun 13;10(6):
pubmed: 34199261
Cell Biol Int. 2020 Aug;44(8):1558-1563
pubmed: 32281696
Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3755-60
pubmed: 21321192
Cell Biol Int. 2022 Dec;46(12):2257-2261
pubmed: 35998257
Ann N Y Acad Sci. 2016 Aug;1378(1):33-40
pubmed: 27385061
Exp Lung Res. 2020 May - Aug;46(6):203-216
pubmed: 32400213
Heliyon. 2019 Dec 03;5(12):e02710
pubmed: 31872097
Cell Cycle. 2022 Oct;21(19):2009-2012
pubmed: 35617133
Am J Physiol Lung Cell Mol Physiol. 2006 Jun;290(6):L1173-82
pubmed: 16428270
Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20470-5
pubmed: 19075233
Cell Mol Life Sci. 2010 Mar;67(6):959-64
pubmed: 20012909
J Clin Invest. 2020 Dec 1;130(12):6218-6221
pubmed: 32976118
Cell Signal. 2022 Nov;99:110432
pubmed: 35933031
Trends Endocrinol Metab. 2011 Aug;22(8):311-7
pubmed: 21530304
Cells. 2022 Mar 19;11(6):
pubmed: 35326496
Endocr Metab Sci. 2021 Jun 30;3:
pubmed: 33796874
EBioMedicine. 2018 Nov;37:557-562
pubmed: 30344124
Endocrine. 2023 Mar;79(3):587-592
pubmed: 36261700
TH Open. 2020 Aug 20;4(3):e173-e174
pubmed: 32844143
Cardiovasc Res. 2018 Jan 1;114(1):35-52
pubmed: 29228169
Cytoskeleton (Hoboken). 2020 May;77(5-6):197-201
pubmed: 32105405
Endocrinology. 2019 Jul 1;160(7):1600-1612
pubmed: 31070727
Endocrines. 2022 Dec;3(4):813-820
pubmed: 36540765
Tissue Barriers. 2019;7(4):1669989
pubmed: 31578921
J Cell Mol Med. 2011 May;15(5):1148-55
pubmed: 20518847
BMJ. 2016 May 23;353:i1585
pubmed: 27217054
J Cell Commun Signal. 2021 Mar;15(1):125-129
pubmed: 33185812
Sci Rep. 2020 Jan 20;10(1):732
pubmed: 31959947
Sci Signal. 2016 Apr 19;9(424):ra40
pubmed: 27095594
Med Gas Res. 2019 Oct-Dec;9(4):208-212
pubmed: 31898605
Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):6067-6074
pubmed: 32123064
J Mol Endocrinol. 2010 Feb;44(2):127-34
pubmed: 19897610
Vitam Horm. 2005;70:1-24
pubmed: 15727800
Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12495-500
pubmed: 16118272
J Biochem Mol Toxicol. 2021 Oct;35(10):e22879
pubmed: 34369038
J Biochem Mol Toxicol. 2019 Oct;33(10):e22380
pubmed: 31339623
Domest Anim Endocrinol. 2002 Jun;22(4):189-200
pubmed: 12044609
Cells. 2020 Oct 21;9(10):
pubmed: 33096674
Nat Clin Pract Endocrinol Metab. 2008 Jan;4(1):33-43
pubmed: 18084344
Cytokine. 2019 Jan;113:427-432
pubmed: 30420201
Prostate. 2010 Jul 1;70(10):1087-93
pubmed: 20232355
Endocrinology. 2022 Dec 19;164(2):
pubmed: 36503995
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6726-6732
pubmed: 32156725
Horm Metab Res. 2020 May;52(5):336-339
pubmed: 32403147
Inflamm Res. 2022 Feb;71(2):183-185
pubmed: 34993559