RHAMM marks proliferative subpopulation of human colorectal cancer stem cells.
CD44
RHAMM
cancer stem cells
colorectal cancer
organoid
Journal
Cancer science
ISSN: 1349-7006
Titre abrégé: Cancer Sci
Pays: England
ID NLM: 101168776
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
revised:
07
03
2023
received:
15
11
2022
accepted:
10
03
2023
medline:
7
7
2023
pubmed:
23
3
2023
entrez:
22
3
2023
Statut:
ppublish
Résumé
The cancer stem cell (CSC) theory features typically rare self-renewing subpopulations that reconstitute the heterogeneous tumor. Identification of molecules that characterize the features of CSCs is a key imperative for further understanding tumor heterogeneity and for the development of novel therapeutic strategies. However, the use of conventional markers of CSCs is still insufficient for the isolation of bona fide CSCs. We investigated organoids that are miniature forms of tumor tissues by reconstructing cellular diversity to identify specific markers to characterize CSCs in heterogeneous tumors. Here, we report that the receptor for hyaluronan-mediated motility (RHAMM) expresses in a subpopulation of CD44+ conventional human colorectal CSC fraction. Single-cell transcriptomics of organoids highlighted RHAMM-positive proliferative cells that revealed distinct characteristics among the various cell types. Prospectively isolated RHAMM+CD44+ cells from the human colorectal cancer tissues showed highly proliferative characteristics with a self-renewal ability in comparison with the other cancer cells. Furthermore, inhibition of RHAMM strongly suppressed organoid formation in vitro and inhibited tumor growth in vivo. Our findings suggest that RHAMM is a potential therapeutic target because it is a specific marker of the proliferative subpopulation within the conventional CSC fraction.
Identifiants
pubmed: 36945114
doi: 10.1111/cas.15795
pmc: PMC10323086
doi:
Substances chimiques
Hyaluronan Receptors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2895-2906Subventions
Organisme : Japan Agency for Medical Research and Development
ID : 16768249
Organisme : Japan Agency for Medical Research and Development
ID : 16770576
Organisme : Japan Society for the Promotion of Science
ID : 15K08970
Organisme : Japan Society for the Promotion of Science
ID : 16747244
Organisme : Japan Society for the Promotion of Science
ID : 16748470
Organisme : Japan Society for the Promotion of Science
ID : 18K07202
Organisme : Japan Society for the Promotion of Science
ID : 19109659
Organisme : Japan Society for the Promotion of Science
ID : 19K16852
Organisme : Shinnihon Foundation of Advanced Medical Treatment Research
Informations de copyright
© 2023 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
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