Determinants of functional synaptic connectivity among amygdala-projecting prefrontal cortical neurons in male mice.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
25 03 2023
25 03 2023
Historique:
received:
25
11
2021
accepted:
13
03
2023
medline:
28
3
2023
entrez:
25
3
2023
pubmed:
26
3
2023
Statut:
epublish
Résumé
The medial prefrontal cortex (mPFC) mediates a variety of complex cognitive functions via its vast and diverse connections with cortical and subcortical structures. Understanding the patterns of synaptic connectivity that comprise the mPFC local network is crucial for deciphering how this circuit processes information and relays it to downstream structures. To elucidate the synaptic organization of the mPFC, we developed a high-throughput optogenetic method for mapping large-scale functional synaptic connectivity in acute brain slices. We show that in male mice, mPFC neurons that project to the basolateral amygdala (BLA) display unique spatial patterns of local-circuit synaptic connectivity, which distinguish them from the general mPFC cell population. When considering synaptic connections between pairs of mPFC neurons, the intrinsic properties of the postsynaptic cell and the anatomical positions of both cells jointly account for ~7.5% of the variation in the probability of connection. Moreover, anatomical distance and laminar position explain most of this fraction in variation. Our findings reveal the factors determining connectivity in the mPFC and delineate the architecture of synaptic connections in the BLA-projecting subnetwork.
Identifiants
pubmed: 36966143
doi: 10.1038/s41467-023-37318-x
pii: 10.1038/s41467-023-37318-x
pmc: PMC10039875
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1667Informations de copyright
© 2023. The Author(s).
Références
Nature. 2009 Feb 26;457(7233):1142-5
pubmed: 19151697
Nat Neurosci. 2019 Dec;22(12):2013-2022
pubmed: 31768051
PLoS Comput Biol. 2017 Mar 14;13(3):e1005423
pubmed: 28291787
Mol Ther. 2003 Jun;7(6):839-50
pubmed: 12788658
Science. 2015 Nov 27;350(6264):aac9462
pubmed: 26612957
iScience. 2018 Oct 26;8:15-28
pubmed: 30268510
J Neurosci. 2006 Apr 19;26(16):4394-405
pubmed: 16624959
Cell Rep. 2015 Dec 15;13(10):2098-106
pubmed: 26670044
Nat Neurosci. 2017 Dec;20(12):1796-1806
pubmed: 29184208
Nature. 2013 Nov 7;503(7474):51-8
pubmed: 24201278
Neuron. 2009 Jan 29;61(2):301-16
pubmed: 19186171
Nat Neurosci. 2005 Nov;8(11):1552-9
pubmed: 16222228
Neuron. 2012 Dec 20;76(6):1057-70
pubmed: 23259943
Nat Biotechnol. 2016 Feb;34(2):199-203
pubmed: 26689543
Nat Neurosci. 2010 Jan;13(1):133-40
pubmed: 20023653
Nature. 2009 Mar 26;458(7237):501-4
pubmed: 19204731
Nat Neurosci. 2017 Jun;20(6):824-835
pubmed: 28436980
Nat Neurosci. 2015 Feb;18(2):170-81
pubmed: 25622573
PLoS Biol. 2005 Mar;3(3):e68
pubmed: 15737062
Nat Commun. 2019 Nov 7;10(1):5067
pubmed: 31699990
Nature. 2016 Jul 21;535(7612):420-4
pubmed: 27409809
Nature. 2011 May 5;473(7345):87-91
pubmed: 21478872
Elife. 2020 Mar 05;9:
pubmed: 32134385
Neuron. 2016 Oct 19;92(2):372-382
pubmed: 27720486
Cell Rep. 2017 Nov 7;21(6):1426-1433
pubmed: 29117549
Front Psychol. 2015 Nov 26;6:1805
pubmed: 26635701
Brain Res. 1996 May 13;720(1-2):211-9
pubmed: 8782914
Elife. 2018 Sep 26;7:
pubmed: 30256194
Transl Psychiatry. 2018 Mar 6;8(1):60
pubmed: 29507292
Neuropsychopharmacology. 2011 Jan;36(2):529-38
pubmed: 20962768
eNeuro. 2016 Feb 02;3(1):
pubmed: 26866055
Sci Adv. 2015 Jul;1(6):
pubmed: 26504902
Nat Commun. 2021 May 17;12(1):2859
pubmed: 34001873
Nature. 2017 Mar 2;543(7643):103-107
pubmed: 28225752
Elife. 2016 Aug 15;5:
pubmed: 27525487
Nat Methods. 2020 Mar;17(3):261-272
pubmed: 32015543
Elife. 2021 May 25;10:
pubmed: 34032211
Nat Commun. 2017 Jul 13;8:16091
pubmed: 28703129
Nature. 2013 Jul 18;499(7458):295-300
pubmed: 23868258
J Neurophysiol. 2019 Oct 1;122(4):1461-1472
pubmed: 31339785
Science. 2016 Sep 9;353(6304):1108
pubmed: 27609883
J Neurosci. 2011 Nov 23;31(47):17269-77
pubmed: 22114293
Nature. 2013 Apr 4;496(7443):96-100
pubmed: 23552948
Neuron. 2018 Dec 19;100(6):1313-1321.e6
pubmed: 30415996
Nat Neurosci. 2015 Nov;18(11):1687-1697
pubmed: 26457553
J Comp Neurol. 2005 Nov 14;492(2):145-77
pubmed: 16196030
Nature. 2015 Jun 18;522(7556):309-14
pubmed: 26053122
Nature. 2012 May 02;486(7401):113-7
pubmed: 22678291
Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3555-60
pubmed: 19221032
J Neurosci. 2012 Apr 18;32(16):5609-19
pubmed: 22514322
Science. 2001 Aug 3;293(5531):868-72
pubmed: 11486089
Neuron. 2013 Apr 24;78(2):364-75
pubmed: 23562541
Neuron. 2015 Jul 15;87(2):437-50
pubmed: 26143660
J Physiol. 1993 Dec;472:615-63
pubmed: 7908327
Nature. 2014 Jan 2;505(7481):92-6
pubmed: 24256726
Eur J Neurosci. 2005 Feb;21(4):1030-44
pubmed: 15787708
J Physiol. 2001 Mar 15;531(Pt 3):807-26
pubmed: 11251060
J Neurosci. 2013 Sep 25;33(39):15333-42
pubmed: 24068800
J Physiol. 2003 Aug 15;551(Pt 1):139-53
pubmed: 12813147
Nat Methods. 2017 Jan;14(1):53-56
pubmed: 27869816
Nature. 2009 Feb 26;457(7233):1133-6
pubmed: 19151698
J Neurosci. 2016 Sep 7;36(36):9391-406
pubmed: 27605614
Cereb Cortex. 2003 Nov;13(11):1124-38
pubmed: 14576205
Nature. 2008 Jul 31;454(7204):600-6
pubmed: 18615015
Annu Rev Neurosci. 2001;24:167-202
pubmed: 11283309
Nature. 2016 Apr 21;532(7599):370-4
pubmed: 27018655
Elife. 2022 Jan 21;11:
pubmed: 35060903
Nat Biotechnol. 2004 Dec;22(12):1567-72
pubmed: 15558047
Nature. 2015 Mar 26;519(7544):460-3
pubmed: 25600268
Cereb Cortex. 2007 Sep;17(9):2204-13
pubmed: 17124287
Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):16807-12
pubmed: 21949377
Front Neurosci. 2007 Oct 15;1(1):19-42
pubmed: 18982117
Curr Biol. 2020 Oct 19;30(20):4063-4070.e2
pubmed: 32822604
J Neurosci. 2014 Jul 16;34(29):9812-6
pubmed: 25031418
J Neurochem. 2019 May;149(4):452-470
pubmed: 30851210
Nat Commun. 2018 Apr 18;9(1):1540
pubmed: 29670095
Neuron. 2015 Jan 7;85(1):68-75
pubmed: 25543458
Brain Struct Funct. 2007 Sep;212(2):149-79
pubmed: 17717690
Brain Res Bull. 2007 Jan 9;71(4):416-27
pubmed: 17208660
J Neurosci. 2011 Mar 9;31(10):3862-70
pubmed: 21389241
J Neurosci. 2009 Nov 4;29(44):13770-84
pubmed: 19889989
Proc Natl Acad Sci U S A. 2009 Sep 1;106(35):15025-30
pubmed: 19706471
J Neurosci. 1999 Apr 15;19(8):2876-86
pubmed: 10191305
Nature. 2015 Feb 19;518(7539):399-403
pubmed: 25652823
Neuron. 2019 Oct 23;104(2):385-401.e3
pubmed: 31371111
J Neurosci. 2012 Sep 12;32(37):12808-19
pubmed: 22973004
Nat Neurosci. 2006 Apr;9(4):534-42
pubmed: 16547512
J Neurosci. 2015 Feb 25;35(8):3607-15
pubmed: 25716859
Nat Neurosci. 2017 Oct;20(10):1377-1383
pubmed: 28825718
Nat Methods. 2012 Dec;9(12):1202-5
pubmed: 23142873
J Neurosci. 2015 Mar 18;35(11):4719-28
pubmed: 25788688
Nature. 2005 Feb 24;433(7028):868-73
pubmed: 15729343
Nat Neurosci. 2006 Dec;9(12):1472-3
pubmed: 17099707
PLoS One. 2013 Apr 24;8(4):e62579
pubmed: 23638117
J Neurosci. 2011 Jul 13;31(28):10380-91
pubmed: 21753015