Towards engineering and production of artificial spider silk using tools of synthetic biology.

artificial spider silk biochemistry biomedical materials biomimetics bio‐inspired materials molecular biophysics proteins reviews spider silk proteins synthetic biology synthetic spider silk

Journal

Engineering biology
ISSN: 2398-6182
Titre abrégé: Eng Biol
Pays: United States
ID NLM: 9918539388906676

Informations de publication

Date de publication:
Mar 2020
Historique:
received: 18 11 2019
revised: 21 01 2020
accepted: 10 02 2020
entrez: 27 3 2023
pubmed: 16 3 2020
medline: 16 3 2020
Statut: epublish

Résumé

Spider silk is one of the strongest biomaterials available in nature. Its mechanical properties make it a good candidate for applications in various fields ranging from protective armour to bandages for wound dressing to coatings for medical implants. Spider silk is formed by an intricate arrangement of spidroins, which are extremely large proteins containing long stretches of repeating segments rich in alanine and glycine. A large amount of research has been directed towards harnessing the spectacular potential of spider silks and using them for different applications. The interdisciplinary approach of synthetic biology is an ideal tool to study these spider silk proteins and work towards the engineering and production of synthetic spider silk. This review aims to highlight the recent progress that has been made in the study of spider silk proteins using different branches of synthetic biology. Here, the authors discuss the different computational approaches, directed evolution techniques and various expression platforms that have been tested for the successful production of spider silk. Future challenges facing the field and possible solutions offered by synthetic biology are also discussed.

Identifiants

DOI: 10.1049/enb.2019.0017 PMID: 36970229 PMC: PMC9996717
pubmed: 36970229
doi: 10.1049/enb.2019.0017
pii: ENB2BF00046
pmc: PMC9996717
doi:

Types de publication

Journal Article Review

Langues

eng

Pagination

1-6

Informations de copyright

© 2020 The Institution of Engineering and Technology.

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Auteurs

Hashwardhan Poddar (H)

Faculty of Science and Engineering, Manchester Institute of Biotechnology, Manchester Synthetic Biology Research Centre SYNBIOCHEM The University of Manchester Manchester M1 7DN UK.

Rainer Breitling (R)

Faculty of Science and Engineering, Manchester Institute of Biotechnology, Manchester Synthetic Biology Research Centre SYNBIOCHEM The University of Manchester Manchester M1 7DN UK.
ORCID: 0000-0001-7173-0922

Eriko Takano (E)

Faculty of Science and Engineering, Manchester Institute of Biotechnology, Manchester Synthetic Biology Research Centre SYNBIOCHEM The University of Manchester Manchester M1 7DN UK.

Classifications MeSH