In-vitro assessment of a novel plant rhizobacterium, Citrobacter freundii, for degrading and biocontrol of food mycotoxin deoxynivalenol.

Animals Mycotoxins / analysis Chromatography, Liquid Citrobacter freundii / metabolism Tandem Mass Spectrometry Bacteria / metabolism Food Contamination / prevention & control
Bacteria Biodegradation Citrobacter freundii Deoxynivalenol (DON) High performance liquid chromatography (HPLC)

Journal

Toxicon : official journal of the International Society on Toxinology
ISSN: 1879-3150
Titre abrégé: Toxicon
Pays: England
ID NLM: 1307333

Informations de publication

Date de publication:
May 2023
Historique:
received: 23 01 2023
revised: 19 03 2023
accepted: 22 03 2023
medline: 18 4 2023
pubmed: 28 3 2023
entrez: 27 3 2023
Statut: ppublish

Résumé

Deoxynivalenol (DON) is one of the most harmful and well-known toxins present in food and animal feed throughout the world. Citrobacter freundii (C. freundii-ON077584), a novel DON-degrading strain, was isolated from rice root-linked soil samples. The degrading properties, including DON concentrations, incubation pH, incubation temperatures, bacterial concentrations, and acid treatment effect on degradation, were evaluated. At pH 7 and an incubation temperature of 37 °C, C. freundii demonstrated the capability to degrade more than 90% of DON. The degraded products of DON were identified as 3-keto-DON and DOM-1, which were confirmed by High Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography hyphenated with Tandem Mass Spectrometry (UPLC-MS/MS) analyses. The mechanism of DON degradation into 3-keto-DON and DOM-1 by this bacterial strain will be further explored to identify and purify novel degrading enzymes that can be cloned to the microorganism and added to the animal feed to degrade the DON in the digestion tract.

Identifiants

DOI: 10.1016/j.toxicon.2023.107095 PMID: 36972839
pubmed: 36972839
pii: S0041-0101(23)00081-8
doi: 10.1016/j.toxicon.2023.107095
pii:
doi:

Substances chimiques

deoxynivalenol JT37HYP23V
deepoxy-deoxynivalenol 88054-24-4
Mycotoxins 0
3-keto-deoxynivalenol 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

107095

Informations de copyright

Copyright © 2023 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Bilal Murtaza (B)

School of Bioengineering, Dalian University of Technology, Dalian, 116024, China. Electronic address: bilalmurtaza50@gmail.com.

Xiaoyu Li (X)

School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China.

Liming Dong (L)

Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing, 100048, China.

Muhammad Kashif Saleemi (MK)

Department of Pathology, University of Agriculture, Faisalabad, Pakistan.

Mazhar Iqbal (M)

National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.

Saima Majeed (S)

Institute of Biochemistry, Biotechnology and Bioinformatics (IBBB), The Islamia University, Bahawalpur, 63100, Pakistan.

Ashiq Ali (A)

Department of Pathology, University of Agriculture, Faisalabad, Pakistan.

Gen Li (G)

School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.

Bowen Jin (B)

School of Bioengineering, Dalian University of Technology, Dalian, 116024, China.

Lili Wang (L)

School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China.

Yongping Xu (Y)

School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Center for Food Safety of Animal Origin, Ministry of Education, Dalian University of Technology, Dalian, 116600, China. Electronic address: xyping@dlut.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › In-vitro assessment of a novel plant...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Mycotoxines › In-vitro assessment of a novel plant...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › In-vitro assessment of a novel plant...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Citrobacter › Citrobacter freundii › In-vitro assessment of a novel plant...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › In-vitro assessment of a novel plant...
questionsmedicales.fr › Bactéries › In-vitro assessment of a novel plant...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Qualité alimentaire › Sécurité des aliments › Contamination des aliments › In-vitro assessment of a novel plant...