A simple, time- and cost-effective, high-throughput depletion strategy for deep plasma proteomics.

Cost-Benefit Analysis Proteomics / methods Proteins

Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
29 03 2023
Historique:
medline: 31 3 2023
entrez: 29 3 2023
pubmed: 30 3 2023
Statut: ppublish

Résumé

We introduce a cost-effective, robust high-throughput-compatible plasma depletion method enabling in-depth profiling of plasma that detects >1300 proteins per run with a throughput of 60 samples per day. The method has been fully validated by processing >3000 samples with no apparent batch effect at a cost for the depletion step of ~$2.5 per sample.

Identifiants

DOI: 10.1126/sciadv.adf9717 PMID: 36989362 PMC: PMC10058233
pubmed: 36989362
doi: 10.1126/sciadv.adf9717
pmc: PMC10058233
doi:

Substances chimiques

Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eadf9717

Références

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Auteurs

Arthur Viode (A)

Department of Pathology, Boston Children's Hospital, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.
ORCID: 0000-0002-3418-734X

Patrick van Zalm (P)

Department of Pathology, Boston Children's Hospital, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.
Department of Neuropsychology and Psychopharmacology, EURON, Faculty of Psychology and Neuroscience, Maastricht University, Maastricht, Netherlands.
ORCID: 0000-0002-0806-3685

Kinga K Smolen (KK)

Harvard Medical School, Boston, MA, USA.
Precision Vaccines Program, Division of Infectious Diseases, Boston Children's Hospital, Boston, MA, USA.
ORCID: 0000-0001-8650-3693

Benoit Fatou (B)

Department of Pathology, Boston Children's Hospital, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.
ORCID: 0000-0001-8650-3693

David Stevenson (D)

Department of Pathology, Boston Children's Hospital, Boston, MA, USA.

Meenakshi Jha (M)

Department of Pathology, Boston Children's Hospital, Boston, MA, USA.
ORCID: 0000-0001-6093-871X

Ofer Levy (O)

Harvard Medical School, Boston, MA, USA.
Precision Vaccines Program, Division of Infectious Diseases, Boston Children's Hospital, Boston, MA, USA.
Broad Institute of MIT and Harvard, Cambridge, MA, USA.
ORCID: 0000-0002-5859-1945

Judith Steen (J)

Department of Neuropsychology and Psychopharmacology, EURON, Faculty of Psychology and Neuroscience, Maastricht University, Maastricht, Netherlands.
F. M. Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA.
ORCID: 0000-0002-8167-0772

Hanno Steen (H)

Department of Pathology, Boston Children's Hospital, Boston, MA, USA.
Harvard Medical School, Boston, MA, USA.
Precision Vaccines Program, Division of Infectious Diseases, Boston Children's Hospital, Boston, MA, USA.
ORCID: 0000-0003-0179-6648

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Classifications MeSH

questionsmedicales.fr › Organisations et économie des soins de santé › Économie › Coûts et analyse des coûts › Analyse coût-bénéfice › A simple, time- and cost-effective,...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › A simple, time- and cost-effective,...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › A simple, time- and cost-effective,...