Refining the Dynamic Network of T2SS Endopilus Tip Heterocomplex Combining cw-EPR and Nitroxide-Gd
Cw-EPR
DEER
endopilus
orthogonal spin labelling
type 2 secretion system
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
01 08 2023
01 08 2023
Historique:
revised:
16
03
2023
received:
07
02
2023
medline:
23
10
2023
pubmed:
1
4
2023
entrez:
31
3
2023
Statut:
ppublish
Résumé
The type 2 secretion system (T2SS) is a bacterial nanomachine composed of an inner membrane assembly platform, an outer membrane pore and a dynamic endopilus. T2SS endopili are organized into a homo-multimeric body formed by the major pilin capped by a heterocomplex of four minor pilins. The first model of the T2SS endopilus was recently released, even if structural dynamics insights are still required to decipher the role of each protein in the full tetrameric complex. Here, we applied continuous-wave and pulse EPR spectroscopy using nitroxide-gadolinium orthogonal labelling strategies to investigate the hetero-oligomeric assembly of the minor pilins. Overall, our data are in line with the endopilus model even if they evidenced conformational flexibility and alternative orientations at local scale of specific regions of minor pilins. The integration of different labelling strategies and EPR experiments demonstrates the pertinence of this approach to investigate protein-protein interactions in such multiprotein heterocomplexes.
Identifiants
pubmed: 36999435
doi: 10.1002/cbic.202300099
doi:
Substances chimiques
Type II Secretion Systems
0
Fimbriae Proteins
147680-16-8
nitroxyl
GFQ4MMS07W
Proteins
0
Spin Labels
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300099Informations de copyright
© 2023 Aix-Marseille Univ, CNRS. ChemBioChem published by Wiley-VCH GmbH.
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