PIEZO1 and PECAM1 interact at cell-cell junctions and partner in endothelial force sensing.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
01 04 2023
01 04 2023
Historique:
received:
30
03
2022
accepted:
14
03
2023
medline:
4
4
2023
entrez:
3
4
2023
pubmed:
4
4
2023
Statut:
epublish
Résumé
Two prominent concepts for the sensing of shear stress by endothelium are the PIEZO1 channel as a mediator of mechanically activated calcium ion entry and the PECAM1 cell adhesion molecule as the apex of a triad with CDH5 and VGFR2. Here, we investigated if there is a relationship. By inserting a non-disruptive tag in native PIEZO1 of mice, we reveal in situ overlap of PIEZO1 with PECAM1. Through reconstitution and high resolution microscopy studies we show that PECAM1 interacts with PIEZO1 and directs it to cell-cell junctions. PECAM1 extracellular N-terminus is critical in this, but a C-terminal intracellular domain linked to shear stress also contributes. CDH5 similarly drives PIEZO1 to junctions but unlike PECAM1 its interaction with PIEZO1 is dynamic, increasing with shear stress. PIEZO1 does not interact with VGFR2. PIEZO1 is required in Ca
Identifiants
pubmed: 37005489
doi: 10.1038/s42003-023-04706-4
pii: 10.1038/s42003-023-04706-4
pmc: PMC10067937
doi:
Substances chimiques
Ion Channels
0
Platelet Endothelial Cell Adhesion Molecule-1
0
Piezo1 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
358Subventions
Organisme : Wellcome Trust
ID : 110044/Z/15/Z
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RG/17/11/33042
Pays : United Kingdom
Organisme : Wellcome Trust (Wellcome)
ID : 204825/Z/16/Z
Organisme : British Heart Foundation
ID : FS/14/22/30734
Pays : United Kingdom
Organisme : British Heart Foundation
ID : FS/17/2/32559
Pays : United Kingdom
Organisme : British Heart Foundation (BHF)
ID : PG/19/2/34084
Informations de copyright
© 2023. The Author(s).
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