The importance of unambiguous cell origin determination in neuronal repopulation studies.
Cell biology
Molecular biology
Neuroscience
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
21 Apr 2023
21 Apr 2023
Historique:
medline:
4
4
2023
entrez:
3
4
2023
pubmed:
4
4
2023
Statut:
epublish
Résumé
Neuronal repopulation achieved through transplantation or transdifferentiation from endogenous sources holds tremendous potential for restoring function in chronic neurodegenerative disease or acute injury. Key to the evaluation of neuronal engraftment is the definitive discrimination of new or donor neurons from preexisting cells within the host tissue. Recent work has identified mechanisms by which genetically encoded donor cell reporters can be transferred to host neurons through intercellular material transfer. In addition, labeling transplanted and endogenously transdifferentiated neurons through viral vector transduction can yield misexpression in host cells in some circumstances. These issues can confound the tracking and evaluation of repopulated neurons in regenerative experimental paradigms. Using the retina as an example, we discuss common reasons for artifactual labeling of endogenous host neurons with donor cell reporters and suggest strategies to prevent erroneous conclusions based on misidentification of cell origin.
Identifiants
pubmed: 37009209
doi: 10.1016/j.isci.2023.106361
pii: S2589-0042(23)00438-8
pmc: PMC10060674
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
106361Subventions
Organisme : NEI NIH HHS
ID : R01 EY026942
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY033022
Pays : United States
Organisme : NEI NIH HHS
ID : U24 EY033269
Pays : United States
Informations de copyright
© 2023 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Invest Ophthalmol Vis Sci. 2011 Mar 01;52(3):1539-47
pubmed: 21245394
Exp Eye Res. 2010 Feb;90(2):308-15
pubmed: 19961848
Stem Cell Res Ther. 2015 Sep 14;6:161
pubmed: 26364954
Front Neural Circuits. 2018 Mar 06;12:17
pubmed: 29559897
Nature. 2012 May 3;485(7396):99-103
pubmed: 22522934
Nat Biotechnol. 2013 Aug;31(8):741-7
pubmed: 23873086
Nat Commun. 2016 Nov 30;7:13537
pubmed: 27901042
Front Cell Dev Biol. 2022 Sep 19;10:914386
pubmed: 36200040
Oncol Rep. 2019 Dec;42(6):2486-2498
pubmed: 31578590
Nat Commun. 2016 Oct 04;7:13028
pubmed: 27701381
Nature. 2006 Nov 9;444(7116):203-7
pubmed: 17093405
Nat Clin Pract Neurol. 2007 Nov;3(11):590-1
pubmed: 17876349
Nat Commun. 2016 Oct 04;7:13029
pubmed: 27701378
EMBO J. 2021 Nov 15;40(22):e107264
pubmed: 34494680
J Comp Neurol. 2011 Mar 1;519(4):599-620
pubmed: 21246546
Invest Ophthalmol Vis Sci. 2018 Mar 1;59(3):1571-1576
pubmed: 29625481
J Clin Invest. 2021 Feb 1;131(3):
pubmed: 33529169
Stem Cell Reports. 2022 Aug 9;17(8):1824-1841
pubmed: 35905738
Stem Cell Reports. 2022 Dec 13;17(12):2690-2703
pubmed: 36368332
Cell Stem Cell. 2017 Mar 2;20(3):374-384.e5
pubmed: 28089909
Stem Cells Dev. 2019 Oct 15;28(20):1365-1375
pubmed: 31580778
Front Cell Dev Biol. 2022 Mar 31;10:830382
pubmed: 35433694
Sci Adv. 2022 Nov 25;8(47):eabq7219
pubmed: 36417510
Mol Ther Methods Clin Dev. 2021 Mar 10;21:180-198
pubmed: 33816648
Stem Cell Reports. 2018 Feb 13;10(2):406-421
pubmed: 29307580
iScience. 2022 Oct 07;25(11):105308
pubmed: 36388952
Stem Cell Res Ther. 2022 Aug 20;13(1):430
pubmed: 35987845
Eur Neuropsychopharmacol. 2019 Dec;29(12):1312-1320
pubmed: 31753777
Stem Cell Reports. 2021 Jan 12;16(1):149-167
pubmed: 33382979
Invest Ophthalmol Vis Sci. 2021 Jul 1;62(9):5
pubmed: 34232261
Nature. 2019 Oct 4;:
pubmed: 33009522
Stem Cells. 2017 Apr;35(4):932-939
pubmed: 27977075
Proc Natl Acad Sci U S A. 2022 Nov 29;119(48):e2206829119
pubmed: 36409915
Exp Eye Res. 2021 May;206:108545
pubmed: 33753089
Stem Cells. 2010 Nov;28(11):1997-2007
pubmed: 20857496
Front Immunol. 2021 May 12;12:621007
pubmed: 34054796
J Clin Invest. 2022 Jun 15;132(12):
pubmed: 35482419
EMBO Rep. 2021 Nov 4;22(11):e53732
pubmed: 34494703
Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):354-9
pubmed: 23248312
Invest Ophthalmol Vis Sci. 2021 Oct 4;62(13):26
pubmed: 34705025
Nat Commun. 2016 Feb 04;7:10472
pubmed: 26843334