Detection of Anaplasma phagocytophilum in fetal and placental tissue of bovine abortions and perinatal mortalities.
Anaplasma phagocytophilum
abortion
cattle
diagnosis
tick-borne fever
Journal
The Veterinary record
ISSN: 2042-7670
Titre abrégé: Vet Rec
Pays: England
ID NLM: 0031164
Informations de publication
Date de publication:
07 Oct 2023
07 Oct 2023
Historique:
revised:
07
03
2023
received:
15
09
2022
accepted:
16
03
2023
medline:
23
10
2023
pubmed:
5
4
2023
entrez:
4
4
2023
Statut:
ppublish
Résumé
Anaplasma phagocytophilum is a tick-borne zoonotic bacterium that is the aetiologic pathogen of tick-borne fever (TBF) in ruminants. In clinical bovine cases of TBF, abortion and stillbirth may be observed. However, in this regard, the pathophysiology of TBF has not yet been completely elucidated, and no clear guidelines to diagnose A. phagocytophilum-related abortions and perinatal mortalities (APM) are available. This exploratory study aimed to investigate the presence of A. phagocytophilum in bovine cases of APM and determine whether placental or fetal spleen tissue has the greatest sensitivity for A. phagocytophilum identification. The placenta and fetal spleen of 150 late-term bovine APM cases were analysed using real-time PCR to detect A. phagocytophilum. A total of 2.7% of sampled placentas were positive for A. phagocytophilum, while none of the fetal spleen samples was. No histopathology to detect associated lesions was performed. Consequently, no evidence of causality between the detection of A. phagocytophilum and APM events could be achieved. The detection of A. phagocytophilum suggests a potential role of this pathogen in bovine APM, and placental tissue seems to be the most suitable tissue for its identification.
Sections du résumé
BACKGROUND
BACKGROUND
Anaplasma phagocytophilum is a tick-borne zoonotic bacterium that is the aetiologic pathogen of tick-borne fever (TBF) in ruminants. In clinical bovine cases of TBF, abortion and stillbirth may be observed. However, in this regard, the pathophysiology of TBF has not yet been completely elucidated, and no clear guidelines to diagnose A. phagocytophilum-related abortions and perinatal mortalities (APM) are available.
METHODS
METHODS
This exploratory study aimed to investigate the presence of A. phagocytophilum in bovine cases of APM and determine whether placental or fetal spleen tissue has the greatest sensitivity for A. phagocytophilum identification. The placenta and fetal spleen of 150 late-term bovine APM cases were analysed using real-time PCR to detect A. phagocytophilum.
RESULTS
RESULTS
A total of 2.7% of sampled placentas were positive for A. phagocytophilum, while none of the fetal spleen samples was.
LIMITATIONS
CONCLUSIONS
No histopathology to detect associated lesions was performed. Consequently, no evidence of causality between the detection of A. phagocytophilum and APM events could be achieved.
CONCLUSION
CONCLUSIONS
The detection of A. phagocytophilum suggests a potential role of this pathogen in bovine APM, and placental tissue seems to be the most suitable tissue for its identification.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2880Subventions
Organisme : Federal Agency for the Safety of the Food Chain
Organisme : Sanitary Fund
Organisme : Fonds voor Wetenschappelijk Onderzoek-Vlaanderen (FWO, Research Foundation, Flanders)
ID : 12Y5220N
Informations de copyright
© 2023 British Veterinary Association.
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