Hydrogel armed with Bmp2 mRNA-enriched exosomes enhances bone regeneration.
Bmp2 mRNA
CP05
Engineered exosome
Gene therapy
Hydrogel
Osteogenesis
Journal
Journal of nanobiotechnology
ISSN: 1477-3155
Titre abrégé: J Nanobiotechnology
Pays: England
ID NLM: 101152208
Informations de publication
Date de publication:
05 Apr 2023
05 Apr 2023
Historique:
received:
21
09
2022
accepted:
24
03
2023
medline:
7
4
2023
entrez:
5
4
2023
pubmed:
6
4
2023
Statut:
epublish
Résumé
Sustained release of bioactive BMP2 (bone morphogenetic protein-2) is important for bone regeneration, while the intrinsic short half-life of BMP2 at protein level cannot meet the clinical need. In this study, we aimed to design Bmp2 mRNA-enriched engineered exosomes, which were then loaded into specific hydrogel to achieve sustained release for more efficient and safe bone regeneration. Bmp2 mRNA was enriched into exosomes by selective inhibition of translation in donor cells, in which NoBody (non-annotated P-body dissociating polypeptide, a protein that inhibits mRNA translation) and modified engineered BMP2 plasmids were co-transfected. The derived exosomes were named Exo Together, the proposed Exo
Sections du résumé
BACKGROUND
BACKGROUND
Sustained release of bioactive BMP2 (bone morphogenetic protein-2) is important for bone regeneration, while the intrinsic short half-life of BMP2 at protein level cannot meet the clinical need. In this study, we aimed to design Bmp2 mRNA-enriched engineered exosomes, which were then loaded into specific hydrogel to achieve sustained release for more efficient and safe bone regeneration.
RESULTS
RESULTS
Bmp2 mRNA was enriched into exosomes by selective inhibition of translation in donor cells, in which NoBody (non-annotated P-body dissociating polypeptide, a protein that inhibits mRNA translation) and modified engineered BMP2 plasmids were co-transfected. The derived exosomes were named Exo
CONCLUSIONS
CONCLUSIONS
Together, the proposed Exo
Identifiants
pubmed: 37020301
doi: 10.1186/s12951-023-01871-w
pii: 10.1186/s12951-023-01871-w
pmc: PMC10075167
doi:
Substances chimiques
Delayed-Action Preparations
0
Hydrogels
0
RNA, Messenger
0
Bone Morphogenetic Protein 2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
119Subventions
Organisme : Sichuan Science and Technology Program
ID : 2020YJ0297
Organisme : National Natural Science Foundation of China
ID : 81700930
Organisme : Shaanxi Science and Technology Research and Development Project
ID : 2021SF-105
Informations de copyright
© 2023. The Author(s).
Références
Nat Chem Biol. 2017 Feb;13(2):174-180
pubmed: 27918561
Nat Methods. 2016 Apr 28;13(5):405-14
pubmed: 27123816
Curr Atheroscler Rep. 2022 May;24(5):307-321
pubmed: 35364795
Biomater Adv. 2022 May;136:212789
pubmed: 35929321
Mater Sci Eng C Mater Biol Appl. 2018 Oct 1;91:806-816
pubmed: 30033316
J Nanobiotechnology. 2022 Jun 14;20(1):279
pubmed: 35701788
BMC Biotechnol. 2021 Mar 10;21(1):20
pubmed: 33691652
Front Mol Biosci. 2021 Jun 08;8:682581
pubmed: 34169095
Nature. 2015 Jan 1;517(7532):33-8
pubmed: 25409156
Adv Sci (Weinh). 2020 Jun 08;7(14):2000542
pubmed: 32714762
Wiley Interdiscip Rev RNA. 2011 Mar-Apr;2(2):277-98
pubmed: 21957010
J Control Release. 2014 Dec 10;195:72-85
pubmed: 25094032
Nano Lett. 2022 Apr 13;22(7):2691-2701
pubmed: 35298182
Cell. 2005 May 6;121(3):411-23
pubmed: 15882623
BioDrugs. 2006;20(1):1-11
pubmed: 16573347
Nature. 1990 Jun 7;345(6275):544-7
pubmed: 2348862
Nano Res. 2023;16(1):672-691
pubmed: 35818566
Biomaterials. 2021 May;272:120718
pubmed: 33838528
BMC Biol. 2016 Jun 13;14:46
pubmed: 27296830
Sci Adv. 2022 Feb 18;8(7):eabl6242
pubmed: 35171668
Bioact Mater. 2022 Feb 28;16:271-284
pubmed: 35386320
ACS Nano. 2020 Sep 22;14(9):11973-11984
pubmed: 32897692
Nature. 2015 Oct 15;526(7573):351-60
pubmed: 26469046
ACS Nano. 2020 Oct 27;14(10):12732-12748
pubmed: 32931251
J Nanobiotechnology. 2022 Mar 15;20(1):135
pubmed: 35292020
Nature. 1975 Jan 31;253(5490):374-5
pubmed: 163011
Acta Biomater. 2020 Jun;109:182-194
pubmed: 32305445
Adv Sci (Weinh). 2018 Oct 12;5(12):1801037
pubmed: 30581705
Nat Commun. 2018 Apr 3;9(1):1305
pubmed: 29610454
Nat Methods. 2018 Jan;15(1):81-89
pubmed: 29131164
Nature. 2012 Sep 6;489(7414):133-6
pubmed: 22955625
Tissue Eng Part A. 2021 Nov;27(21-22):1422-1433
pubmed: 33882718
J Control Release. 2016 Oct 10;239:137-48
pubmed: 27586186
J Control Release. 2019 Jul 10;305:165-175
pubmed: 31121277
Nature. 1975 May 1;255(5503):33-7
pubmed: 165427
J Control Release. 2022 May;345:494-511
pubmed: 35337940
Nat Commun. 2022 Jul 27;13(1):4350
pubmed: 35896523
Science. 1999 Sep 24;285(5436):2084-8
pubmed: 10523208
Science. 2020 Feb 7;367(6478):
pubmed: 32029601
Int J Nanomedicine. 2022 Aug 16;17:3633-3653
pubmed: 35996527
J Mater Sci Mater Med. 2017 Dec 01;29(1):2
pubmed: 29196819
J Biomed Nanotechnol. 2020 Dec 1;16(12):1667-1686
pubmed: 33485397
Front Bioeng Biotechnol. 2021 Mar 18;9:628137
pubmed: 33816449
Adv Healthc Mater. 2013 Jul;2(7):934-9
pubmed: 23296589
Transl Res. 2021 Oct;236:1-16
pubmed: 33964474
Acta Pharm Sin B. 2016 Jul;6(4):287-96
pubmed: 27471669
Nat Rev Genet. 2022 May;23(5):265-280
pubmed: 34983972
Science. 2012 Jun 1;336(6085):1124-8
pubmed: 22654050
J Control Release. 2012 Jul 20;161(2):554-65
pubmed: 22123560
Stem Cell Res Ther. 2020 Jan 28;11(1):38
pubmed: 31992369
Adv Drug Deliv Rev. 2013 Mar;65(3):357-67
pubmed: 22820532
Int J Mol Sci. 2019 Mar 20;20(6):
pubmed: 30897788
Sci Transl Med. 2018 Jun 6;10(444):
pubmed: 29875202
Leukemia. 2021 Mar;35(3):752-763
pubmed: 32632095
Biomaterials. 2016 May;87:131-146
pubmed: 26923361
Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13368-73
pubmed: 15326289