Systematic analysis of human antibody response to ebolavirus glycoprotein shows high prevalence of neutralizing public clonotypes.
CP: Immunology
Ebola
adaptive immunity
antibodies
human
monoclonal
repertoire
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
25 04 2023
25 04 2023
Historique:
received:
12
07
2022
revised:
19
08
2022
accepted:
22
03
2023
medline:
4
10
2023
pubmed:
9
4
2023
entrez:
8
4
2023
Statut:
ppublish
Résumé
Understanding the human antibody response to emerging viral pathogens is key to epidemic preparedness. As the size of the B cell response to a pathogenic-virus-protective antigen is poorly defined, we perform deep paired heavy- and light-chain sequencing in Ebola virus glycoprotein (EBOV-GP)-specific memory B cells, allowing analysis of the ebolavirus-specific antibody repertoire both genetically and functionally. This approach facilitates investigation of the molecular and genetic basis for the evolution of cross-reactive antibodies by elucidating germline-encoded properties of antibodies to EBOV and identification of the overlap between antibodies in the memory B cell and serum repertoire. We identify 73 public clonotypes of EBOV, 20% of which encode antibodies with neutralization activity and capacity to protect mice in vivo. This comprehensive analysis of the public and private antibody repertoire provides insight into the molecular basis of the humoral immune response to EBOV GP, which informs the design of vaccines and improved therapeutics.
Identifiants
pubmed: 37029928
pii: S2211-1247(23)00381-9
doi: 10.1016/j.celrep.2023.112370
pmc: PMC10556194
mid: NIHMS1895593
pii:
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Glycoproteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112370Subventions
Organisme : NIAID NIH HHS
ID : T32 AI138932
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI142785
Pays : United States
Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests E.D. and B.J.D. are employees of Integral Molecular, and B.J.D.is a shareholder in that company. J.E.C. has served as a consultant for Luna Labs USA, Merck Sharp & Dohme, Emergent Biosolutions, and GlaxoSmithKline; is a member of the scientific advisory board of Meissa Vaccines; is a former member of the scientific advisory board of Gigagen (Grifols); and is the founder of IDBiologics. The laboratory of J.E.C. received unrelated sponsored research agreements from AstraZeneca, Takeda, and IDBiologics during the conduct of the study.
Références
Immunity. 2018 Aug 21;49(2):363-374.e10
pubmed: 30029854
Immunity. 2021 Apr 13;54(4):769-780.e6
pubmed: 33823129
Cell. 2016 Jan 28;164(3):392-405
pubmed: 26806128
Nat Commun. 2020 Oct 15;11(1):5208
pubmed: 33060581
Cell. 2016 Jul 28;166(3):609-623
pubmed: 27453470
J Infect Dis. 2018 Nov 22;218(suppl_5):S612-S626
pubmed: 29860496
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W34-40
pubmed: 23671333
Cell Rep. 2021 Aug 24;36(8):109604
pubmed: 34411541
Curr Opin Virol. 2019 Feb;34:149-159
pubmed: 30884330
Science. 2020 Aug 28;369(6507):1119-1123
pubmed: 32661058
N Engl J Med. 2019 Dec 12;381(24):2365-2366
pubmed: 31774948
Bioinformatics. 2017 Aug 15;33(16):2594-2595
pubmed: 28398468
Nat Methods. 2019 Nov;16(11):1153-1160
pubmed: 31591578
Nature. 2014 Oct 2;514(7520):47-53
pubmed: 25171469
Nat Med. 2020 Sep;26(9):1422-1427
pubmed: 32651581
J Infect Dis. 2018 Nov 22;218(suppl_5):S528-S536
pubmed: 30010811
N Engl J Med. 2020 May 7;382(19):1832-1842
pubmed: 32441897
Nat Commun. 2021 Jun 21;12(1):3815
pubmed: 34155209
Mol Syst Biol. 2011 Oct 11;7:539
pubmed: 21988835
Nat Biomed Eng. 2020 Nov;4(11):1030-1043
pubmed: 32747832
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Nat Med. 2021 Apr;27(4):622-625
pubmed: 33654292
J Struct Biol. 2015 Nov;192(2):216-21
pubmed: 26278980
JCI Insight. 2017 May 4;2(9):
pubmed: 28469084
Nat Methods. 2017 Mar;14(3):290-296
pubmed: 28165473
Virology. 2005 Feb 5;332(1):20-7
pubmed: 15661137
Cell. 2021 Apr 29;184(9):2316-2331.e15
pubmed: 33773105
J Virol. 2004 May;78(10):5458-65
pubmed: 15113924
Cell Host Microbe. 2018 Jun 13;23(6):845-854.e6
pubmed: 29861170
Methods Enzymol. 1996;266:418-27
pubmed: 8743697
Cell. 2019 May 30;177(6):1566-1582.e17
pubmed: 31104840
Leukemia. 2003 Dec;17(12):2257-317
pubmed: 14671650
Cell Host Microbe. 2019 Jan 9;25(1):49-58.e5
pubmed: 30629918
Cell. 2017 May 18;169(5):878-890.e15
pubmed: 28525755
Sci Immunol. 2021 Feb 23;6(56):
pubmed: 33622975
Cell. 2015 Jun 4;161(6):1280-92
pubmed: 26004070
Cell Host Microbe. 2020 Mar 11;27(3):418-427.e4
pubmed: 32059794
Nat Commun. 2019 Mar 21;10(1):1321
pubmed: 30899025
Nature. 2019 Feb;566(7744):398-402
pubmed: 30760926
Virus Res. 2005 Oct;113(1):16-25
pubmed: 16139097
Science. 2016 Mar 4;351(6277):1078-83
pubmed: 26912366
MAbs. 2015;7(2):403-12
pubmed: 25621616
Immunity. 2021 Sep 14;54(9):2159-2166.e6
pubmed: 34464596
Immunity. 2020 Feb 18;52(2):388-403.e12
pubmed: 32023489
Biol Proced Online. 2004;6:23-34
pubmed: 15103397
Front Immunol. 2021 Jul 16;12:706757
pubmed: 34335620
Nat Protoc. 2009;4(3):372-84
pubmed: 19247287
J Infect Dis. 2018 Nov 22;218(suppl_5):S418-S422
pubmed: 30060231
J Struct Biol. 2005 Oct;152(1):36-51
pubmed: 16182563
Mol Pathol. 2002 Apr;55(2):98-101
pubmed: 11950958
BMC Bioinformatics. 2020 Jul 16;21(1):314
pubmed: 32677886
Cell Rep. 2021 Apr 13;35(2):108984
pubmed: 33852862
Nature. 2014 Dec 18;516(7531):418-22
pubmed: 25296253
Nature. 2019 Feb;566(7744):393-397
pubmed: 30664748
J Infect Dis. 1998 Sep;178(3):651-61
pubmed: 9728532
Nat Med. 2019 Oct;25(10):1589-1600
pubmed: 31591605
Cell Rep. 2019 Apr 2;27(1):172-186.e7
pubmed: 30943399
J Clin Invest. 2021 Aug 2;131(15):
pubmed: 34156974
Nat Microbiol. 2021 Oct;6(10):1233-1244
pubmed: 34548634
J Virol. 2016 Mar 28;90(8):3890-3901
pubmed: 26819310
J Virol. 2019 Apr 3;93(8):
pubmed: 30728263
Science. 2016 Mar 18;351(6279):1339-42
pubmed: 26917593
Science. 2016 Mar 18;351(6279):1343-6
pubmed: 26917592
J Exp Med. 2016 Apr 4;213(4):469-81
pubmed: 27022144
Microbiol Spectr. 2015 Feb;3(1):AID-0027-2014
pubmed: 26104564