Effects of Prunus Tomentosa Thumb Total Flavones on adjuvant arthritis in rats and regulation of autophagy.
Prunus Tomentosa Thumb Total Flavones
autophagy
inflammatory factors
Journal
Technology and health care : official journal of the European Society for Engineering and Medicine
ISSN: 1878-7401
Titre abrégé: Technol Health Care
Pays: Netherlands
ID NLM: 9314590
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
2
5
2023
pubmed:
12
4
2023
entrez:
11
4
2023
Statut:
ppublish
Résumé
Rheumatoid arthritis (RA) is a slow in taking effect systemic autoimmune disease. Prunus Tomentosa Thumb Total Flavones (PTTTF) has anti-inflammatory and antioxidant properties. The purpose of this study is to the PTTTF on adjuvant arthritis (AA) in rats and to explore the mechanism of autophagy. Adjuvant arthritis model was established in rats. The cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-17 (IL-17), tumor necrosis factor (TNF-α) of rat synovial tissue were determined by RT-PCR. The histopathological varieties of knee joints in AA rats were observed by HE staining. The expressions of autophagy-related proteins ATG5, ATG7, ATG12, Beclin1, Lc3II and Bcl-2 in rat synovial tissue were determined by Western Blotting. PTTTF (50, 100, 200 mg/kg) significantly inhibited inflammation in rats (P< 0.01). PTTTF significantly inhibited inflammatory factor COX in rat synovial tissue. COX-2, IL-1β, IL-6, IL-17, TNF-α expression (P< 0.05); PTTTF can significantly improve the pathological damage of rat knee joint PTTTF and can significantly inhibited the expression of autophagy-related proteins in rat synovium (P< 0.05 ). PTTTF can inhibit adjuvant arthritis in rats and can inhibit the expression of autophagy-related proteins ATG5, ATG7, ATG12, Beclin1, Lc3II and Bcl-2.
Sections du résumé
BACKGROUND
BACKGROUND
Rheumatoid arthritis (RA) is a slow in taking effect systemic autoimmune disease. Prunus Tomentosa Thumb Total Flavones (PTTTF) has anti-inflammatory and antioxidant properties.
OBJECTIVE
OBJECTIVE
The purpose of this study is to the PTTTF on adjuvant arthritis (AA) in rats and to explore the mechanism of autophagy.
METHODS
METHODS
Adjuvant arthritis model was established in rats. The cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-17 (IL-17), tumor necrosis factor (TNF-α) of rat synovial tissue were determined by RT-PCR. The histopathological varieties of knee joints in AA rats were observed by HE staining. The expressions of autophagy-related proteins ATG5, ATG7, ATG12, Beclin1, Lc3II and Bcl-2 in rat synovial tissue were determined by Western Blotting.
RESULTS
RESULTS
PTTTF (50, 100, 200 mg/kg) significantly inhibited inflammation in rats (P< 0.01). PTTTF significantly inhibited inflammatory factor COX in rat synovial tissue. COX-2, IL-1β, IL-6, IL-17, TNF-α expression (P< 0.05); PTTTF can significantly improve the pathological damage of rat knee joint PTTTF and can significantly inhibited the expression of autophagy-related proteins in rat synovium (P< 0.05 ).
CONCLUSION
CONCLUSIONS
PTTTF can inhibit adjuvant arthritis in rats and can inhibit the expression of autophagy-related proteins ATG5, ATG7, ATG12, Beclin1, Lc3II and Bcl-2.
Identifiants
pubmed: 37038787
pii: THC236012
doi: 10.3233/THC-236012
pmc: PMC10200214
doi:
Substances chimiques
Interleukin-17
0
Interleukin-6
0
Tumor Necrosis Factor-alpha
0
Beclin-1
0
Cyclooxygenase 2
EC 1.14.99.1
Autophagy-Related Proteins
0
Proto-Oncogene Proteins c-bcl-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
123-136Références
Connect Tissue Res. 2019 Jul;60(4):358-366
pubmed: 30477351
Curr Drug Targets. 2018;19(9):1009-1017
pubmed: 27550202
Clin Transl Med. 2017 Dec;6(1):24
pubmed: 28748360
Int Immunopharmacol. 2020 Aug;85:106642
pubmed: 32470883
Cells. 2020 Apr 03;9(4):
pubmed: 32260219
Sci Rep. 2016 Sep 09;6:32928
pubmed: 27611176
Am J Transl Res. 2018 Mar 15;10(3):762-770
pubmed: 29636866
Front Immunol. 2021 Dec 24;12:809806
pubmed: 35003139
Inflammation. 2021 Feb;44(1):1-12
pubmed: 32954452
Life Sci. 2019 Jun 1;226:164-172
pubmed: 30970265
Int Immunopharmacol. 2020 Jul;84:106570
pubmed: 32413739
Chem Biol Interact. 2019 Dec 1;314:108839
pubmed: 31563593
Mediators Inflamm. 2017;2017:7623145
pubmed: 28255205
Cell. 2008 Jan 11;132(1):27-42
pubmed: 18191218
Histochem Cell Biol. 2017 Jun;147(6):695-705
pubmed: 28097431
Nature. 2019 Nov;575(7781):203-209
pubmed: 31666698
Lancet. 2016 Oct 22;388(10055):2023-2038
pubmed: 27156434
J Enzyme Inhib Med Chem. 2021 Dec;36(1):450-461
pubmed: 33557646
Viruses. 2019 Aug 23;11(9):
pubmed: 31450758
Am J Transl Res. 2017 May 15;9(5):2065-2076
pubmed: 28559961
Front Pharmacol. 2020 May 15;11:568
pubmed: 32499694
Oncotarget. 2017 Feb 28;8(9):15420-15430
pubmed: 28053286
Exp Ther Med. 2018 Sep;16(3):2670-2676
pubmed: 30186500
Autophagy. 2018;14(2):243-251
pubmed: 29165043
Bioessays. 2020 Nov;42(11):e2000122
pubmed: 32851706