Effects of Follicle-Stimulating Hormone on Human Sperm Motility In Vitro.
FSH
FSHR
sperm motility
spermatozoa
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
31 Mar 2023
31 Mar 2023
Historique:
received:
31
01
2023
revised:
17
03
2023
accepted:
29
03
2023
medline:
14
4
2023
entrez:
13
4
2023
pubmed:
14
4
2023
Statut:
epublish
Résumé
To evaluate whether the follicle-stimulating hormone (FSH) receptor (FSHR) is expressed in human spermatozoa and the effects of FSH incubation on sperm function. Twenty-four Caucasian men were recruited. Thirteen patients had asthenozoospermia, and the remaining 11 had normal sperm parameters (controls). After confirming FSHR expression, spermatozoa from patients and controls were incubated with increasing concentrations of human purified FSH (hpFSH) to reassess FSHR expression and localization and to evaluate progressive and total sperm motility, the mitochondrial membrane potential, and protein kinase B (AKT) 473 and 308 phosphorylation. FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa. Its localization does not differ between patients and controls. Incubation with hpFSH at a concentration of 30 mIU/mL appeared to increase FSHR expression mainly in patients. Incubation of human spermatozoa with hpFSH overall resulted in an overall deterioration of both progressive and total motility in patients and controls and worse mitochondrial function only in controls. Finally, incubation with FSH increased AKT
Identifiants
pubmed: 37047508
pii: ijms24076536
doi: 10.3390/ijms24076536
pmc: PMC10095528
pii:
doi:
Substances chimiques
Follicle Stimulating Hormone
9002-68-0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Follicle Stimulating Hormone, Human
0
Receptors, FSH
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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