Expansion of human megakaryocyte-biased hematopoietic stem cells by biomimetic Microniche.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 04 2023
18 04 2023
Historique:
received:
14
11
2022
accepted:
04
04
2023
medline:
20
4
2023
pubmed:
19
4
2023
entrez:
18
04
2023
Statut:
epublish
Résumé
Limited numbers of available hematopoietic stem cells (HSCs) limit the widespread use of HSC-based therapies. Expansion systems for functional heterogenous HSCs remain to be optimized. Here, we present a convenient strategy for human HSC expansion based on a biomimetic Microniche. After demonstrating the expansion of HSC from different sources, we find that our Microniche-based system expands the therapeutically attractive megakaryocyte-biased HSC. We demonstrate scalable HSC expansion by applying this strategy in a stirred bioreactor. Moreover, we identify that the functional human megakaryocyte-biased HSCs are enriched in the CD34
Identifiants
pubmed: 37072407
doi: 10.1038/s41467-023-37954-3
pii: 10.1038/s41467-023-37954-3
pmc: PMC10113370
doi:
Substances chimiques
Antigens, CD34
0
Leukocyte Common Antigens
EC 3.1.3.48
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2207Informations de copyright
© 2023. The Author(s).
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