Immunohistochemical and ultrastructural review of six cases previously diagnosed as null cell PitNETs.
Electron microscopy
Null cell pituitary neuroendocrine tumor
Transcription factors
Ultrastructure
Journal
Brain tumor pathology
ISSN: 1861-387X
Titre abrégé: Brain Tumor Pathol
Pays: Japan
ID NLM: 9716507
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
04
03
2023
accepted:
07
04
2023
medline:
3
7
2023
pubmed:
19
4
2023
entrez:
18
04
2023
Statut:
ppublish
Résumé
Pituitary neuroendocrine tumors (PitNETs) lacking lineage affiliation are termed "null cell" PitNETs (NCTs). NCTs are characterized as being immunonegative for pituitary hormones as well as transcription factors. We analyzed the ultrastructure and immunohistochemistry of six hormone-negative and transcription factor (TPIT, PIT1, SF1)-negative PitNETs, with less than 1% immunoreactive cells. Histologically, three cases presented a perivascular pattern and pseudorosettes; the other three showed a solid pattern with oncocytic changes. Electron microscopic examination revealed poorly differentiated tumor cells with sparsely scattered secretory granules and intracellular organelles in all null cell tumors when compared with hormone-positive PitNETs. Two cases harbored a honeycomb Golgi (HG) structure, and three oncocytic tumors showed mitochondrial accumulation. The two HG cases were immunopositive for newly obtained TPIT (CL6251) and showed some adrenocorticotropic hormone-positive cells, while the remaining four were diffusely immunopositive for GATA3, with two SF1-positive cases identified in subsequent immunostaining. Thus, these six cases may be classified as two sparsely granulated corticotroph PitNETs, two gonadotroph PitNETs with SF1 re-staining, and two likely gonadotroph PitNETs with GATA3 immunostaining. No "true NCT" was detected among 1071 PitNETs, demonstrating the importance of precise diagnosis following the most recent criteria to improve therapeutic success.
Identifiants
pubmed: 37072666
doi: 10.1007/s10014-023-00462-9
pii: 10.1007/s10014-023-00462-9
doi:
Substances chimiques
Transcription Factors
0
Hormones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
158-162Subventions
Organisme : MEXT KAKENHI
ID : 19185472
Informations de copyright
© 2023. The Author(s), under exclusive licence to The Japan Society of Brain Tumor Pathology.
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