Restoration of aberrant gene expression of monocytes in systemic lupus erythematosus via a combined transcriptome-reversal and network-based drug repurposing strategy.


Journal

BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258

Informations de publication

Date de publication:
18 Apr 2023
Historique:
received: 24 08 2022
accepted: 27 03 2023
medline: 20 4 2023
pubmed: 19 4 2023
entrez: 18 04 2023
Statut: epublish

Résumé

Monocytes -key regulators of the innate immune response- are actively involved in the pathogenesis of systemic lupus erythematosus (SLE). We sought to identify novel compounds that might serve as monocyte-directed targeted therapies in SLE. We performed mRNA sequencing in monocytes from 15 patients with active SLE and 10 healthy individuals. Disease activity was assessed with the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2 K). Leveraging the drug repurposing platforms iLINCS, CLUE and L1000CDS Application of two independent - a transcriptome-reversal and a network-based -drug repurposing strategies uncovered novel agents that might remedy transcriptional disturbances of monocytes in SLE.

Sections du résumé

BACKGROUND BACKGROUND
Monocytes -key regulators of the innate immune response- are actively involved in the pathogenesis of systemic lupus erythematosus (SLE). We sought to identify novel compounds that might serve as monocyte-directed targeted therapies in SLE.
RESULTS RESULTS
We performed mRNA sequencing in monocytes from 15 patients with active SLE and 10 healthy individuals. Disease activity was assessed with the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2 K). Leveraging the drug repurposing platforms iLINCS, CLUE and L1000CDS
CONCLUSIONS CONCLUSIONS
Application of two independent - a transcriptome-reversal and a network-based -drug repurposing strategies uncovered novel agents that might remedy transcriptional disturbances of monocytes in SLE.

Identifiants

pubmed: 37072752
doi: 10.1186/s12864-023-09275-8
pii: 10.1186/s12864-023-09275-8
pmc: PMC10114456
doi:

Substances chimiques

NF-kappa B 0
MicroRNAs 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

207

Subventions

Organisme : European Research Council
ID : 742390
Pays : International

Informations de copyright

© 2023. The Author(s).

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Auteurs

Dimitrios Nikolakis (D)

Amsterdam Institute for Gastroenterology Endocrinology and Metabolism, Department of Gastroenterology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Meibergdreef 9, Amsterdam, 1105 AZ, The Netherlands.
Department of Rheumatology and Clinical Immunology, Amsterdam Rheumatology & Immunology Center (ARC), Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
Amsterdam Institute for Infection & Immunity, Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
Onassis Foundation, Athens, Greece.

Panagiotis Garantziotis (P)

Laboratory of Autoimmunity and Inflammation, Center for Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Department Rheumatology and Immunology, Hannover Medical School, Hannover, Germany.

George Sentis (G)

Laboratory of Autoimmunity and Inflammation, Center for Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.

Antonis Fanouriakis (A)

Rheumatology and Clinical Immunology Unit, Department of Internal Medicine, Attikon University Hospital, Athens, 4th, Greece.
Department of Propaedeutic Internal Medicine, "Laiko" General Hospital, Athens, Greece.
Joint Academic Rheumatology Program, National and Kapodistrian University of Athens Medical School, Athens, Greece.

George Bertsias (G)

Department of Rheumatology and Clinical Immunology, Medical School, University Hospital of Heraklion, University of Crete, Heraklion, Greece.
Institute of Molecular Biology and Biotechnology-FORTH, Heraklion, Greece.

Eleni Frangou (E)

Laboratory of Autoimmunity and Inflammation, Center for Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Department of Nephrology, Limassol General Hospital, Limassol, Cyprus.
Medical School, University of Nicosia, Nicosia, Cyprus.

Dionysis Nikolopoulos (D)

Laboratory of Autoimmunity and Inflammation, Center for Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
Rheumatology and Clinical Immunology Unit, Department of Internal Medicine, Attikon University Hospital, Athens, 4th, Greece.

Aggelos Banos (A)

Laboratory of Autoimmunity and Inflammation, Center for Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.

Dimitrios T Boumpas (DT)

Laboratory of Autoimmunity and Inflammation, Center for Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, Athens, Greece. boumpasd@uoc.gr.
Rheumatology and Clinical Immunology Unit, Department of Internal Medicine, Attikon University Hospital, Athens, 4th, Greece. boumpasd@uoc.gr.
Joint Academic Rheumatology Program, National and Kapodistrian University of Athens Medical School, Athens, Greece. boumpasd@uoc.gr.

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