CARD8 Inflammasome Activation by HIV-1 Protease.

Inflammasomes / metabolism CARD Signaling Adaptor Proteins / metabolism Apoptosis Regulatory Proteins / metabolism HIV Protease
CARD8 inflammasome Caspase-1 (CASP1) HIV-1 Protease Pyroptosis

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
medline: 21 4 2023
pubmed: 19 4 2023
entrez: 19 04 2023
Statut: ppublish

Résumé

The pattern recognition receptor CARD8 is an inflammasome sensor for intracellular HIV-1 protease activity. Previously, the only method for studying the CARD8 inflammasome has been through utilizing DPP8/DPP9 inhibitors including Val-boroPro (VbP) to modestly and nonspecifically activate the CARD8 inflammasome. The identification of HIV-1 protease as a target for sensing by CARD8 has opened the door for a new method of studying the underlying mechanism of CARD8 inflammasome activation. Additionally, triggering the CARD8 inflammasome offers a promising strategy for reducing HIV-1 latent reservoirs. Here we describe the methods to study CARD8 sensing of HIV-1 protease activity through non-nucleoside reverse transcriptase inhibitor (NNRTI)-mediated pyroptosis of HIV-1-infected immune cells and through an HIV and CARD8 co-transfection model.

Identifiants

DOI: 10.1007/978-1-0716-3040-2_6 PMID: 37074642
pubmed: 37074642
doi: 10.1007/978-1-0716-3040-2_6
doi:

Substances chimiques

Inflammasomes 0
CARD Signaling Adaptor Proteins 0
p16 protease, Human immunodeficiency virus 1 EC 3.4.23.-
Apoptosis Regulatory Proteins 0
HIV Protease EC 3.4.23.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

67-79

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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Auteurs

Kolin M Clark (KM)

Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Saint Louis, MO, USA.

Qiankun Wang (Q)

Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Saint Louis, MO, USA.

Liang Shan (L)

Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, Saint Louis, MO, USA. liang.shan@wustl.edu.
Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, Saint Louis, MO, USA. liang.shan@wustl.edu.

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Classifications MeSH

questionsmedicales.fr › Structures macromoléculaires › Complexes multiprotéiques › Inflammasomes › CARD8 Inflammasome Activation by HIV-1 Protease.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › Protéines adaptatrices de signalisation CARD › CARD8 Inflammasome Activation by HIV-1 Protease.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines régulatrices de l'apoptose › CARD8 Inflammasome Activation by HIV-1 Protease.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales non structurales › Protéases virales › Protéase du VIH › CARD8 Inflammasome Activation by HIV-1 Protease.