Resveratrol improved mitochondrial biogenesis by activating SIRT1/PGC-1α signal pathway in SAP.
Sirtuin 1
/ metabolism
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
/ metabolism
Animals
Resveratrol
/ pharmacology
Rats
Signal Transduction
/ drug effects
Organelle Biogenesis
Male
Pancreatitis
/ drug therapy
NLR Family, Pyrin Domain-Containing 3 Protein
/ metabolism
Inflammasomes
/ metabolism
Mitochondria
/ metabolism
Rats, Sprague-Dawley
Pyroptosis
/ drug effects
Cell Line
Mitochondrial biogenesis
NLRP3 inflammasomes- pyroptosis axis
Resveratrol
SIRT1/PGC-1α
Severe acute pancreatitis
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
31 Oct 2024
31 Oct 2024
Historique:
received:
08
02
2024
accepted:
16
10
2024
medline:
1
11
2024
pubmed:
1
11
2024
entrez:
1
11
2024
Statut:
epublish
Résumé
NLRP3 inflammasomes- pyroptosis axis is activated by microcirculation dysfunction and touched off severe acute pancreatitis (SAP). Activation of PGC-1α can improve microcirculation dysfunction by promoting mitochondrial biogenesis. Resveratrol (RSV), one typical SIRT1 agonist, possesses the ability of alleviating SAP and activing PGC-1α. Therefore, the study was designated to explore whether the protective effect of RSV in SAP was though suppressing NLRP3 inflammasomes- pyroptosis axis via advancing SIRT1/PGC-1α-dependent mitochondrial biogenesis. The models of SAP were induced by treating with sodium taurodeoxycholate in rats and AR42J cells. The pathological injury, water content (dry/wet ratio) and microcirculation function of pancreas, activity of lipase and amylase were used to evaluate pancreatic damage. The expression of inflammatory cytokine was measured by ELISA and RT-PCR. The damage of mitochondrial was evaluated by measuring the changes in Mitochondrial Membrane Potential (ΔΨm), mitochondrial ROS, ATP content and MDA as well as relocation of mtDNA and the activity of SOD and GSH. The expressions of NLRP3 inflammasomes- pyroptosis axis proteins were detected by Western blotting as well as SIRT1/PGC-1α/NRF1/TFAM pathway protein. Moreover, the modification of PGC-1α was measured by co-immunoprecipitation. The results displayed that RSV can significantly improve the damage of pancreas and mitochondrial, decrease the expression of pro-inflammatory factor and the activation of NLRP3 inflammasomes- pyroptosis axis, promote the expression of an-inflammatory factor and the deacetylation of PGC-1α together with facilitating SIRT1/PGC-1α-mediating mitochondrial biogenesis. Therefore, the protective effect of RSV in SAP is though inactivation of NLRP3 inflammasomes- pyroptosis axis via promoting mitochondrial biogenesis in a SIRT1/PGC-1α-dependent manner.
Identifiants
pubmed: 39482340
doi: 10.1038/s41598-024-76825-9
pii: 10.1038/s41598-024-76825-9
doi:
Substances chimiques
Sirtuin 1
EC 3.5.1.-
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
0
Resveratrol
Q369O8926L
Ppargc1a protein, rat
0
Sirt1 protein, rat
EC 3.5.1.-
NLR Family, Pyrin Domain-Containing 3 Protein
0
Inflammasomes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
26216Subventions
Organisme : Sichuan Provincial Department of Science and Technology
ID : 2022YFS0331
Informations de copyright
© 2024. The Author(s).
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