Phytochemical Estimation and Therapeutic Amelioration of Aesculus hippocastanum L. Seeds Ethanolic Extract in Gastric Ulcer in Rats Possibly by Inhibiting Prostaglandin Synthesis.
Rats
Animals
Stomach Ulcer
/ chemically induced
Antioxidants
/ pharmacology
Ranitidine
/ adverse effects
Aesculus
Ulcer
/ drug therapy
Quercetin
Plant Extracts
/ pharmacology
Indomethacin
/ therapeutic use
Glutathione
Superoxide Dismutase
Rutin
/ adverse effects
Prostaglandins
/ adverse effects
Phytochemicals
/ therapeutic use
Aesculus hippocastanum L.
gastro-protective activity
liquid chromatography and mass spectroscopy analysis
pharmacological action
physicochemical analysis
phytochemical
Journal
Chinese journal of integrative medicine
ISSN: 1993-0402
Titre abrégé: Chin J Integr Med
Pays: China
ID NLM: 101181180
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
accepted:
27
07
2022
medline:
31
8
2023
pubmed:
20
4
2023
entrez:
20
04
2023
Statut:
ppublish
Résumé
To quantify phytochemicals using liquid chromatography and mass spectroscopy (LCMS) analysis and explore the therapeutic effect of Aesculus hippocastanum L. (AH) seeds ethanolic extract against gastric ulcers in rats. Preliminary phytochemical testing and LCMS analysis were performed according to standard methods. For treatment, the animals were divided into 7 groups including normal control, ulcer control, self-healing, AH seeds low and high doses, ranitidine and per se groups. Rats were orally administered with 10 mg/kg of indomethacin, excluding the normal control group (which received 1% carboxy methyl cellulose) and the per se group (received 200 mg/kg AH seeds extract). The test group rats were then given 2 doses of AH seeds extract (100 and 200 mg/kg, respectively), while the standard group was given ranitidine (50 mg/kg). On the 11th day, rats in all groups were sacrificed, and their stomach was isolated to calculate the ulcer index, and other parameters such as blood prostaglandin (PGE The phytochemical examination shows that the AH seeds contain alkaloids, flavonoids, saponins, phenolic components, and glycosides. LCMS analysis confirms the presence of quercetin and rutin. The AH seeds extract showed significant improvement in gastric mucosa conditions after indomethacin-induced gastric lesions (P<0.01). Further marked improvement in blood PGE LCMS report confirms the presence of quercetin and rutin in AH seeds ethanolic extract. The therapeutic effect of AH seeds extract against indomethacin-induced ulcer in rat model indicated the regenerated membrane integrity, with improved cellular functions and mucus thickness. Further, improved antioxidant enzyme level would help to reduce PGE
Identifiants
pubmed: 37079159
doi: 10.1007/s11655-023-3734-9
pii: 10.1007/s11655-023-3734-9
doi:
Substances chimiques
Antioxidants
0
Ranitidine
884KT10YB7
Quercetin
9IKM0I5T1E
Plant Extracts
0
Indomethacin
XXE1CET956
Glutathione
GAN16C9B8O
Superoxide Dismutase
EC 1.15.1.1
Rutin
5G06TVY3R7
Prostaglandins
0
Phytochemicals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
818-824Informations de copyright
© 2023. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.
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