Clonally expanded PD-1-expressing T cells are enriched in synovial fluid of juvenile idiopathic arthritis patients.


Journal

European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201

Informations de publication

Date de publication:
Jul 2023
Historique:
revised: 23 03 2023
received: 30 08 2022
accepted: 17 04 2023
medline: 17 7 2023
pubmed: 22 4 2023
entrez: 22 04 2023
Statut: ppublish

Résumé

Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic condition in childhood. The disease etiology remains largely unknown; however, a key role in JIA pathogenesis is surely mediated by T cells. T-lymphocytes activity is controlled via signals, known as immune checkpoints. Delivering an inhibitory signal or blocking a stimulatory signal to achieve immune suppression is critical in autoimmune diseases. However, the role of immune checkpoints in chronic inflammation and autoimmunity must still be deciphered. In this study, we investigated at the single-cell level the feature of T cells in JIA chronic inflammation, both at the transcriptome level via single-cell RNA sequencing and at the protein level by flow cytometry. We found that despite the heterogeneity in the composition of synovial CD4

Identifiants

pubmed: 37086046
doi: 10.1002/eji.202250162
doi:

Substances chimiques

Programmed Cell Death 1 Receptor 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2250162

Subventions

Organisme : Department of Experimental and Clinical Medicine of University of Florence

Informations de copyright

© 2023 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.

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Auteurs

Anna Vanni (A)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.

Alessio Mazzoni (A)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.
Flow Cytometry Diagnostic Center and Immunotherapy, Careggi University Hospital, Florence, Tuscany, Italy.

Roberto Semeraro (R)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.

Manuela Capone (M)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.

Patrick Maschmeyer (P)

Institute of Health (BIH) at Charité, Universitätsmedizin Berlin, Berlin, Berlin, Germany.
Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Institute for Medical Systems Biology (BIMSB), Berlin, Berlin, Germany.
Department of Hematology, Oncology and Cancer Immunology, Charité-Universitätsmedizin Berlin, Berlin, Berlin, Germany.

Giulia Lamacchia (G)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.

Lorenzo Salvati (L)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.

Alberto Carnasciali (A)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.

Parham Farahvachi (P)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.

Teresa Giani (T)

AOU Meyer, Florence, Tuscany, Italy.

Gabriele Simonini (G)

AOU Meyer, Florence, Tuscany, Italy.

Giovanni Filocamo (G)

Pediatric Rheumatology, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Milano IT and University of Milan, Milan, Lombardy, Italy.

Micol Romano (M)

University of Western Ontario, London, Ontario, Canada.

Francesco Liotta (F)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.
Immunology and Cell Therapy Unit, Careggi University Hospital, Florence, Tuscany, Italy.

Mir-Farzin Mashreghi (MF)

Deutsches Rheuma-Forschungszentrum (DRFZ), Institute of the Leibniz Association, Berlin, Berlin, Germany.

Lorenzo Cosmi (L)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.
Immunoallergology Unit, Careggi University Hospital, Florence, Tuscany, Italy.

Rolando Cimaz (R)

Department of Clinical Sciences and Community Health, Research Center for Adult and Pediatric Rheumatic Diseases, University of Milan, Milan, Lombardy, Italy.

Alberto Magi (A)

Department of Information Engineering, University of Florence, Florence, Tuscany, Italy.

Laura Maggi (L)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.

Francesco Annunziato (F)

Department of Experimental and Clinical Medicine, University of Florence, Florence, Tuscany, Italy.
Flow Cytometry Diagnostic Center and Immunotherapy, Careggi University Hospital, Florence, Tuscany, Italy.

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