Prominent epigenetic and transcriptomic changes in CD4
CD4+
CD8+
Methylation
Modules
Multiple sclerosis
Networks
Pregnancy
T cells
Transcriptomics
Journal
Journal of neuroinflammation
ISSN: 1742-2094
Titre abrégé: J Neuroinflammation
Pays: England
ID NLM: 101222974
Informations de publication
Date de publication:
27 Apr 2023
27 Apr 2023
Historique:
received:
17
10
2022
accepted:
17
04
2023
medline:
1
5
2023
pubmed:
28
4
2023
entrez:
27
4
2023
Statut:
epublish
Résumé
Multiple sclerosis (MS) is a neuroinflammatory disease in which pregnancy leads to a temporary amelioration in disease activity as indicated by the profound decrease in relapses rate during the 3rd trimester of pregnancy. CD4 Women with MS and healthy controls were longitudinally sampled before, during (1st, 2nd and 3rd trimesters) and after pregnancy. DNA methylation array and RNA sequencing were performed on paired CD4 Both DNA methylation and RNA sequencing revealed a prominent regulation, mostly peaking in the 3rd trimester and reversing post-partum, thus mirroring the clinical course with improvement followed by a worsening in disease activity. This rebound pattern was found to represent a general adaptation of the maternal immune system, with only minor differences between MS and controls. By using a network-based approach, we highlighted several genes at the core of this pregnancy-induced regulation, which were found to be enriched for genes and pathways previously reported to be involved in MS. Moreover, these pathways were enriched for in vitro stimulated genes and pregnancy hormones targets. This study represents, to our knowledge, the first in-depth investigation of the methylation and expression changes in peripheral CD4
Sections du résumé
BACKGROUND
BACKGROUND
Multiple sclerosis (MS) is a neuroinflammatory disease in which pregnancy leads to a temporary amelioration in disease activity as indicated by the profound decrease in relapses rate during the 3rd trimester of pregnancy. CD4
METHODS
METHODS
Women with MS and healthy controls were longitudinally sampled before, during (1st, 2nd and 3rd trimesters) and after pregnancy. DNA methylation array and RNA sequencing were performed on paired CD4
RESULTS
RESULTS
Both DNA methylation and RNA sequencing revealed a prominent regulation, mostly peaking in the 3rd trimester and reversing post-partum, thus mirroring the clinical course with improvement followed by a worsening in disease activity. This rebound pattern was found to represent a general adaptation of the maternal immune system, with only minor differences between MS and controls. By using a network-based approach, we highlighted several genes at the core of this pregnancy-induced regulation, which were found to be enriched for genes and pathways previously reported to be involved in MS. Moreover, these pathways were enriched for in vitro stimulated genes and pregnancy hormones targets.
CONCLUSION
CONCLUSIONS
This study represents, to our knowledge, the first in-depth investigation of the methylation and expression changes in peripheral CD4
Identifiants
pubmed: 37106402
doi: 10.1186/s12974-023-02781-2
pii: 10.1186/s12974-023-02781-2
pmc: PMC10134602
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
98Subventions
Organisme : Neuroförbundet
ID : F2018-0052
Organisme : Forskningsrådet i Sydöstra Sverige
ID : FORSS-315121
Organisme : Stiftelsen för Strategisk Forskning
ID : SB16-0011
Organisme : Vetenskapsrådet
ID : 2018-02776
Informations de copyright
© 2023. The Author(s).
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