α-Lactam Electrophiles for Covalent Chemical Biology.

Activity-Based Protein Profiling Hydrolases Inhibitors Lactams Small Ring Systems

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
26 06 2023
Historique:
received: 22 03 2023
medline: 19 6 2023
pubmed: 28 4 2023
entrez: 28 4 2023
Statut: ppublish

Résumé

Electrophilic groups are one of the key pillars of contemporary chemical biology and medicinal chemistry. For instance, 3-membered N-heterocyclic compounds-such as aziridines, azirines, and oxaziridines-possess unique electronic and structural properties which underlie their potential and applicability as covalent tools. The α-lactams are also members of this group of compounds, however, their utility within the field remains unexplored. Here, we demonstrate an α-lactam reagent (AM2) that is tolerant to aqueous buffers while being reactive towards biologically relevant nucleophiles. Interestingly, carboxylesterases 1 and 2 (CES1/2), both serine hydrolases with key roles in endo- and xenobiotic metabolism, were found as primary covalent targets for AM2 in HepG2 liver cancer cells. All in all, this study constitutes the starting point for the further development and exploration of α-lactam-based electrophilic probes in covalent chemical biology.

Identifiants

pubmed: 37114559
doi: 10.1002/anie.202304142
doi:

Substances chimiques

Lactams 0
Heterocyclic Compounds 0
Azirines 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202304142

Informations de copyright

© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

Références

J. Singh, R. C. Petter, T. A. Baillie, A. Whitty, Nat. Rev. Drug Discovery 2011, 10, 307-317.
E. De Vita, Future Med. Chem. 2021, 13, 193-210.
T. Zhang, J. M. Hatcher, M. Teng, N. S. Gray, M. Kostic, Cell Chem. Biol. 2019, 26, 1486-1500.
K. M. Backus, B. E. Correia, K. M. Lum, S. Forli, B. D. Horning, G. E. González-Páez, S. Chatterjee, B. R. Lanning, J. R. Teijaro, A. J. Olson, D. W. Wolan, B. F. Cravatt, Nature 2016, 534, 570-574.
A. M. Roberts, D. K. Miyamoto, T. R. Huffman, L. A. Bateman, A. N. Ives, D. Akopian, M. J. Heslin, C. M. Contreras, M. Rape, C. F. Skibola, D. K. Nomura, ACS Chem. Biol. 2017, 12, 899-904.
E. Resnick, A. Bradley, J. Gan, A. Douangamath, T. Krojer, R. Sethi, P. P. Geurink, A. Aimon, G. Amitai, D. Bellini, J. Bennett, M. Fairhead, O. Fedorov, R. Gabizon, J. Gan, J. Guo, A. Plotnikov, N. Reznik, G. F. Ruda, L. Díaz-Sáez, V. M. Straub, T. Szommer, S. Velupillai, D. Zaidman, Y. Zhang, A. R. Coker, C. G. Dowson, H. M. Barr, C. Wang, K. V. M. Huber, P. E. Brennan, H. Ovaa, F. Von Delft, N. London, J. Am. Chem. Soc. 2019, 141, 8951-8968.
A. Keeley, L. Petri, P. Ábrányi-Balogh, G. M. Keserű, Drug Discovery Today 2020, 25, 983-996.
M. B. Cordon, K. M. Jacobsen, C. S. Nielsen, P. Hjerrild, T. B. Poulsen, ChemBioChem 2022, 23, e202100253.
Y. Liu, M. P. Patricelli, B. F. Cravatt, Proc. Natl. Acad. Sci. USA 1999, 96, 14694-14699.
B. F. Cravatt, A. T. Wright, J. W. Kozarich, Annu. Rev. Biochem. 2008, 77, 383-414.
E. Weerapana, C. Wang, G. M. Simon, F. Richter, S. Khare, M. B. D. Dillon, D. A. Bachovchin, K. Mowen, D. Baker, B. F. Cravatt, Nature 2010, 468, 790-795.
P. R. A. Zanon, F. Yu, P. Z. Musacchio, L. Lewald, M. Zollo, K. Krauskopf, D. Mrdović, P. Raunft, T. E. Maher, M. Cigler, C. J. Chang, K. Lang, F. D. Toste, A. I. Nesvizhskii, S. M. Hacker, ChemRxiv preprint 2021, https://doi.org/10.26434/chemrxiv.14186561.v1.
P. R. A. Zanon, L. Lewald, S. M. Hacker, Angew. Chem. Int. Ed. 2020, 59, 2829-2836.
B. K. Hansen, C. J. Loveridge, S. Thyssen, G. J. Wørmer, A. D. Nielsen, J. Palmfeldt, M. Johannsen, T. B. Poulsen, Angew. Chem. Int. Ed. 2019, 58, 3533-3537.
G. J. Wørmer, B. K. Hansen, J. Palmfeldt, T. B. Poulsen, Angew. Chem. Int. Ed. 2019, 58, 11918-11922.
M. Gehringer, S. A. Laufer, J. Med. Chem. 2019, 62, 5673-5724.
K. Bach, B. L. H. Beerkens, P. R. A. Zanon, S. M. Hacker, ACS Cent. Sci. 2020, 6, 546-554.
M. Ahangarpour, I. Kavianinia, P. A. Hume, P. W. R. Harris, M. A. Brimble, J. Am. Chem. Soc. 2022, 144, 13652-13662.
A. Istrate, M. B. Geeson, C. D. Navo, B. B. Sousa, M. C. Marques, R. J. Taylor, T. Journeaux, S. R. Oehler, M. R. Mortensen, M. J. Deery, A. D. Bond, F. Corzana, G. Jiménez-Osés, G. J. L. Bernardes, J. Am. Chem. Soc. 2022, 144, 10396-10406.
R. N. Reddi, E. Resnick, A. Rogel, B. V. Rao, R. Gabizon, K. Goldenberg, N. Gurwicz, D. Zaidman, A. Plotnikov, H. Barr, Z. Shulman, N. London, J. Am. Chem. Soc. 2021, 143, 4979-4992.
M. J. S. A. Silva, H. Faustino, J. A. S. Coelho, M. V. Pinto, A. Fernandes, I. Compañón, F. Corzana, G. Gasser, P. M. P. Gois, Angew. Chem. Int. Ed. 2021, 60, 10850-10857.
C. E. Stieger, Y. Park, M. A. R. de Geus, D. Kim, C. Huhn, J. S. Slenczka, P. Ochtrop, J. M. Müchler, R. Süssmuth, J. Broichhagen, M.-H. Baik, C. Hackenberger, Angew. Chem. Int. Ed. 2022, 61, e202205348.
K. C. Tang, S. M. Maddox, K. M. Backus, M. Raj, Chem. Sci. 2022, 13, 763-774.
B. P. Rempel, S. G. Withers, Glycobiology 2008, 18, 570-586.
L. M. McGregor, M. L. Jenkins, C. Kerwin, J. E. Burke, K. M. Shokat, Biochemistry 2017, 56, 3178-3183.
S. Lin, X. Yang, S. Jia, A. M. Weeks, M. Hornsby, P. S. Lee, R. V. Nichiporuk, A. T. Iavarone, J. A. Wells, F. D. Toste, C. J. Chang, Science 2017, 355, 597-602.
A. H. Christian, S. Jia, W. Cao, P. Zhang, A. T. Meza, M. S. Sigman, C. J. Chang, F. D. Toste, J. Am. Chem. Soc. 2019, 141, 12657-12662.
N. Ma, J. Hu, Z. M. Zhang, W. Liu, M. Huang, Y. Fan, X. Yin, J. Wang, K. Ding, W. Ye, Z. Li, J. Am. Chem. Soc. 2020, 142, 6051-6059.
E. Smith, I. Collins, Future Med. Chem. 2015, 7, 159-183.
A. V. West, G. Muncipinto, H. Y. Wu, A. C. Huang, M. T. Labenski, L. H. Jones, C. M. Woo, J. Am. Chem. Soc. 2021, 143, 6691-6700.
I. Lengyel, J. C. Sheehan, Angew. Chem. Int. Ed. Engl. 1968, 7, 25-36.
C. Hubschwerlen, in Compr. Med. Chem. II, Elsevier Ltd, Amsterdam, 2007, pp. 479-518.
M. Benitez, Y. D. Wang, I. Lengyel, M. Fitzsimmons, V. Cesare, J. Heterocycl. Chem. 2018, 55, 2877-2882.
E. R. Talaty, M. M. Yusoff, Chem. Commun. 1998, 985-986.
R. V. Hoffman, Z. Zhao, A. Costales, D. Clarke, J. Org. Chem. 2002, 67, 5284-5294.
D. J. Tantillo, K. N. Houk, R. V. Hoffman, J. Tao, J. Org. Chem. 1999, 64, 3830-3837.
I. Lengyel, V. Cesare, S. Chen, T. Taldone, Heterocycles 2002, 57, 677-695.
E. R. Talaty, C. M. Utermoehlen, J. Chem. Soc. D 1970, 473-474.
H. K. Box, K. G. Upul Kumarasinghe, R. R. Nareddy, G. Akurathi, A. Chakraborty, B. Raji, G. B. Rowland, Tetrahedron 2014, 70, 9709-9717.
J. C. Sheehan, I. A. Lengyel, J. Am. Chem. Soc. 1964, 86, 746-747.
J. C. Sheehan, J. H. Beeson, J. Am. Chem. Soc. 1967, 89, 362-366.
F. Maran, J. Am. Chem. Soc. 1993, 115, 6557-6563.
A. D. Cohen, B. M. Showalter, J. P. Toscano, Org. Lett. 2004, 6, 401-403.
I. Lengyel, V. Cesare, T. Taldone, Tetrahedron 2004, 60, 1107-1124.
I. Lengyel, D. B. Uliss, Chem. Commun. 1968, 1621-1622.
I. Lengyel, D. B. Uliss, R. V. Mark, J. Org. Chem. 1970, 35, 4077-4084.
J. C. Sheehan, M. M. Nafissi-V, J. Am. Chem. Soc. 1969, 91, 1176-1178.
D. Wang, L. Zou, Q. Jin, J. Hou, G. Ge, L. Yang, Acta Pharm. Sin. B 2018, 8, 699-712.
C. Wang, D. Abegg, B. G. Dwyer, A. Adibekian, ChemBioChem 2019, 20, 2212-2216.
B. F. Krippendorff, R. Neuhaus, P. Lienau, A. Reichel, W. Huisinga, J. Biomol. Screening 2009, 14, 913-923.
E. Mons, S. Roet, R. Q. Kim, M. P. C. Mulder, Curr. Protoc. 2022, 2, e419.
J. A. Crow, V. Bittles, A. Borazjani, P. M. Potter, M. K. Ross, Biochem. Pharmacol. 2012, 84, 1215-1222.
A. Tuley, W. Fast, Biochemistry 2018, 57, 3326-3337.
Y. Q. Song, Q. Jin, D. D. Wang, J. Hou, L. W. Zou, G. B. Ge, Chem.-Biol. Interact. 2021, 345, 109566.
M. K. Ross, A. Borazjani, R. Wang, J. Allen Crow, S. Xie, Arch. Biochem. Biophys. 2012, 522, 44-56.
V. Cesare, T. M. Lyons, I. Lengyel, Synthesis 2002, 1716-1720.
G. F. Makhaeva, S. V. Lushchekina, N. P. Boltneva, O. G. Serebryakova, N. V. Kovaleva, E. V. Rudakova, N. A. Elkina, E. V. Shchegolkov, Y. V. Burgart, T. S. Stupina, A. A. Terentiev, E. V. Radchenko, V. A. Palyulin, V. I. Saloutin, S. O. Bachurin, R. J. Richardson, Eur. J. Med. Chem. 2021, 218, 113385.
A. Singh, M. Gao, M. W. Beck, RSC Med. Chem. 2021, 12, 1142-1153.
E. Dominguez, A. Galmozzi, J. W. Chang, K. L. Hsu, J. Pawlak, W. Li, C. Godio, J. Thomas, D. Partida, S. Niessen, P. E. O'Brien, A. P. Russell, M. J. Watt, D. K. Nomura, B. F. Cravatt, E. Saez, Nat. Chem. Biol. 2014, 10, 113-121.

Auteurs

Alejandro Mahía (A)

Department of Chemistry, Aarhus University, Langelandsgade 140, 8000, Aarhus C, Denmark.

Anders E Kiib (AE)

Department of Chemistry, Aarhus University, Langelandsgade 140, 8000, Aarhus C, Denmark.

Marija Nisavic (M)

Department of Chemistry, Aarhus University, Langelandsgade 140, 8000, Aarhus C, Denmark.
Department of Clinical Medicine-Research Unit for Molecular Medicine, Aarhus University Hospital, Palle Juul-Jensens Boulevard 82, 8200, Aarhus N, Denmark.

Esben B Svenningsen (EB)

Department of Chemistry, Aarhus University, Langelandsgade 140, 8000, Aarhus C, Denmark.

Johan Palmfeldt (J)

Department of Clinical Medicine-Research Unit for Molecular Medicine, Aarhus University Hospital, Palle Juul-Jensens Boulevard 82, 8200, Aarhus N, Denmark.

Thomas B Poulsen (TB)

Department of Chemistry, Aarhus University, Langelandsgade 140, 8000, Aarhus C, Denmark.

Articles similaires

Biological Evolution History, 20th Century Selection, Genetic History, 19th Century Biology

Nirmatrelvir plus ritonavir reduces COVID-19 hospitalization and prevents long COVID in adult outpatients.

Fatemeh Saheb Sharif-Askari, Hawra Ali Hussain Alsayed, Narjes Saheb Sharif-Askari et al.
1.00
Ritonavir Humans Female Male Middle Aged
Animals Cyclams Benzylamines Hematopoietic Stem Cell Transplantation Granulocyte Colony-Stimulating Factor
Humans Biology Educational Measurement Students Curriculum

Classifications MeSH