NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b.

Dynamics FGF FGF8 FGF8b FGFR Fibroblast growth factor Fibroblast growth factor receptor. Motions Structure

Journal

Biomolecular NMR assignments
ISSN: 1874-270X
Titre abrégé: Biomol NMR Assign
Pays: Netherlands
ID NLM: 101472371

Informations de publication

Date de publication:
06 2023
Historique:
received: 21 02 2023
accepted: 24 04 2023
medline: 2 6 2023
pubmed: 29 4 2023
entrez: 28 4 2023
Statut: ppublish

Résumé

The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the previously reported X-ray data, excluding the N-terminal gN helix, which apparently forms only in complex with the receptor. The reported data provide an NMR starting point for the investigation of FGF8b interaction with its receptors and with potential drugs or inhibitors.

Identifiants

pubmed: 37118562
doi: 10.1007/s12104-023-10132-8
pii: 10.1007/s12104-023-10132-8
pmc: PMC10232571
doi:

Substances chimiques

Fibroblast Growth Factor 8 148997-75-5
Protein Isoforms 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

135-142

Informations de copyright

© 2023. The Author(s).

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Auteurs

Bruno Hargittay (B)

Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe University, Max-von-Laue-Str. 7, 60438, Frankfurt/Main, Germany.

Konstantin S Mineev (KS)

Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe University, Max-von-Laue-Str. 7, 60438, Frankfurt/Main, Germany.

Christian Richter (C)

Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe University, Max-von-Laue-Str. 7, 60438, Frankfurt/Main, Germany.

Sridhar Sreeramulu (S)

Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe University, Max-von-Laue-Str. 7, 60438, Frankfurt/Main, Germany.

Hendrik R A Jonker (HRA)

Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe University, Max-von-Laue-Str. 7, 60438, Frankfurt/Main, Germany.

Krishna Saxena (K)

Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe University, Max-von-Laue-Str. 7, 60438, Frankfurt/Main, Germany.
Structural Genomics Consortium, Johann Wolfgang Goethe University, Max-von-Laue-Str. 15, 60438, Frankfurt/Main, Germany.

Harald Schwalbe (H)

Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe University, Max-von-Laue-Str. 7, 60438, Frankfurt/Main, Germany. schwalbe@nmr.uni-frankfurt.de.

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Classifications MeSH