Molecular mechanism of engineered Zymomonas mobilis to furfural and acetic acid stress.
Acetic acid
Furfural
Lignocellulosic biomass
Proteomics
RNA-Seq
Zymomonas mobilis
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
02 May 2023
02 May 2023
Historique:
received:
06
03
2023
accepted:
12
04
2023
medline:
3
5
2023
pubmed:
2
5
2023
entrez:
1
5
2023
Statut:
epublish
Résumé
Acetic acid and furfural (AF) are two major inhibitors of microorganisms during lignocellulosic ethanol production. In our previous study, we successfully engineered Zymomonas mobilis 532 (ZM532) strain by genome shuffling, but the molecular mechanisms of tolerance to inhibitors were still unknown. Therefore, this study investigated the responses of ZM532 and its wild-type Z. mobilis (ZM4) to AF using multi-omics approaches (transcriptomics, genomics, and label free quantitative proteomics). Based on RNA-Seq data, two differentially expressed genes, ZMO_RS02740 (up-regulated) and ZMO_RS06525 (down-regulated) were knocked out and over-expressed through CRISPR-Cas technology to investigate their roles in AF tolerance. Overall, we identified 1865 and 14 novel DEGs in ZM532 and wild-type ZM4. In contrast, 1532 proteins were identified in ZM532 and wild-type ZM4. Among these, we found 96 important genes in ZM532 involving acid resistance mechanisms and survival rates against stressors. Furthermore, our knockout results demonstrated that growth activity and glucose consumption of mutant strains ZM532∆ZMO_RS02740 and ZM4∆ZMO_RS02740 decreased with increased fermentation time from 42 to 55 h and ethanol production up to 58% in ZM532 than that in ZM532∆ZMO_RS02740. Hence, these findings suggest ZMO_RS02740 as a protective strategy for ZM ethanol production under stressful conditions.
Identifiants
pubmed: 37127628
doi: 10.1186/s12934-023-02095-1
pii: 10.1186/s12934-023-02095-1
pmc: PMC10152622
doi:
Substances chimiques
Acetic Acid
Q40Q9N063P
Furaldehyde
DJ1HGI319P
Ethanol
3K9958V90M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
88Subventions
Organisme : National Natural Sciences Foundation of China
ID : 32070036
Informations de copyright
© 2023. The Author(s).
Références
Appl Microbiol Biotechnol. 2019 Mar;103(5):2087-2099
pubmed: 30661108
BMC Microbiol. 2010 May 07;10:135
pubmed: 20459639
Biotechnol Biofuels. 2020 Jan 25;13:15
pubmed: 31998408
Biotechnol Biofuels. 2013 Feb 15;6(1):22
pubmed: 23409974
Biotechnol Biofuels. 2015 Sep 22;8:153
pubmed: 26396591
Access Microbiol. 2019 Sep 25;1(8):e000054
pubmed: 32974554
Biotechnol Biofuels. 2018 May 02;11:125
pubmed: 29743953
Structure. 2016 Dec 6;24(12):2227-2235
pubmed: 27866852
Metab Eng. 2019 Jul;54:301-316
pubmed: 31078792
Acta Physiol (Oxf). 2017 Feb;219(2):409-440
pubmed: 27364602
Mol Microbiol. 2000 Jul;37(2):239-53
pubmed: 10931321
Biochem Biophys Res Commun. 1996 Dec 4;229(1):219-24
pubmed: 8954109
PLoS One. 2018 Dec 17;13(12):e0202238
pubmed: 30557402
BMC Biotechnol. 2017 Aug 1;17(1):63
pubmed: 28764759
Genetics. 1998 Mar;148(3):929-36
pubmed: 9539414
Genetics. 1995 Feb;139(2):1067-76
pubmed: 7713409
Nat Rev Microbiol. 2008 Dec;6(12):893-903
pubmed: 18997824
ACS Infect Dis. 2018 Mar 9;4(3):373-381
pubmed: 29260856
Biotechnol Biofuels. 2019 Jan 05;12:10
pubmed: 30627218
EMBO J. 1998 Jul 15;17(14):3968-80
pubmed: 9670013
BMC Microbiol. 2008 Oct 15;8:181
pubmed: 18922164
Biotechnol Biofuels. 2020 Aug 13;13:144
pubmed: 32817760
FEMS Microbiol Lett. 2014 Dec;361(1):17-24
pubmed: 25251081
Nat Biotechnol. 2010 May;28(5):511-5
pubmed: 20436464
AIMS Microbiol. 2020 Feb 11;6(1):1-31
pubmed: 32226912
Front Microbiol. 2019 Apr 30;10:953
pubmed: 31114568
J Biol Chem. 1992 Feb 5;267(4):2507-11
pubmed: 1370823
Appl Microbiol Biotechnol. 2015 Jun;99(12):5363-71
pubmed: 25895089
Bioinformatics. 2014 May 1;30(9):1236-40
pubmed: 24451626
Biotechnol Biofuels. 2012 Oct 11;5(1):75
pubmed: 23057803
J Chromatogr A. 2015 Oct 30;1418:158-166
pubmed: 26422304
Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10395-400
pubmed: 20484677
Nucleic Acids Res. 2014;42(16):10776-85
pubmed: 25114049
J Biol Chem. 2005 Apr 15;280(15):15370-9
pubmed: 15657035
Mol Microbiol. 2012 Mar;83(5):926-35
pubmed: 22288397
PLoS One. 2015 Apr 23;10(4):e0124563
pubmed: 25905464
Nat Rev Microbiol. 2004 Nov;2(11):898-907
pubmed: 15494746
Biotechnol Biofuels. 2021 Jan 6;14(1):5
pubmed: 33407786
Molecules. 2018 Feb 01;23(2):
pubmed: 29389875
Wiley Interdiscip Rev RNA. 2014 Nov-Dec;5(6):747-63
pubmed: 24944158
Nature. 1997 Jan 9;385(6612):151-4
pubmed: 8990116
Biochim Biophys Acta Biomembr. 2018 Dec;1860(12):2549-2565
pubmed: 30293966
J Bacteriol. 2002 Nov;184(22):6357-66
pubmed: 12399506
Appl Environ Microbiol. 1994 Dec;60(12):4624-6
pubmed: 7811102
Appl Environ Microbiol. 2021 Mar 11;87(7):
pubmed: 33483304
Genome Biol. 2007;8(2):R22
pubmed: 17313685
Biotechnol Biofuels. 2011 Jun 24;4(1):18
pubmed: 21702938
Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656
pubmed: 14665678
Biotechnol Biofuels. 2019 Dec 16;12:288
pubmed: 31890016
Appl Environ Microbiol. 2009 Oct;75(19):6132-41
pubmed: 19684179
J Biotechnol. 2009 Dec;144(4):259-67
pubmed: 19683550
Free Radic Res. 2002 Jan;36(1):13-21
pubmed: 11999699
Cells. 2021 Feb 14;10(2):
pubmed: 33673015
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
BMC Plant Biol. 2021 Feb 2;21(1):71
pubmed: 33530948
Bioinformatics. 2006 May 15;22(10):1172-6
pubmed: 16452114
Biol Open. 2018 Jan 17;7(1):
pubmed: 29242197
Biotechnol Biofuels. 2013 Jul 09;6(1):99
pubmed: 23837621
Bioinformatics. 2010 Jan 1;26(1):136-8
pubmed: 19855105
Front Microbiol. 2014 May 22;5:246
pubmed: 24904559
Nature. 1997 Jun 12;387(6634):667-8
pubmed: 9192889
Biosci Biotechnol Biochem. 2004 Feb;68(2):458-61
pubmed: 14981317
Appl Environ Microbiol. 2020 Nov 10;86(23):
pubmed: 32978126
Biotechnol Rep (Amst). 2019 Mar 21;23:e00328
pubmed: 30984572
Nucleic Acids Res. 2013 Aug;41(14):e140
pubmed: 23716638
Nucleic Acids Res. 2009 Jan;37(1):1-13
pubmed: 19033363
Bioinformatics. 2008 Dec 15;24(24):2849-56
pubmed: 18940824
Front Microbiol. 2019 Apr 17;10:822
pubmed: 31057523
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
Biotechnol Biofuels. 2019 Mar 14;12:52
pubmed: 30911332
Microbiology (Reading). 2012 May;158(Pt 5):1196-1205
pubmed: 22322961
Biotechnol Biofuels. 2014 Sep 30;7(1):140
pubmed: 25298783
Microbiologyopen. 2016 Aug;5(4):626-36
pubmed: 27061463
Appl Microbiol Biotechnol. 2012 Mar;93(6):2513-8
pubmed: 22350321
Bioinformatics. 2001 Dec;17(12):1123-30
pubmed: 11751220
Appl Microbiol Biotechnol. 2015 Feb;99(4):2009-22
pubmed: 25582559
Appl Microbiol Biotechnol. 2012 Jul;95(1):189-99
pubmed: 22592554
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Annu Rev Microbiol. 1988;42:359-93
pubmed: 2849372
Appl Microbiol Biotechnol. 2015 Jul;99(13):5739-48
pubmed: 25935346
J Biol Chem. 2000 Nov 10;275(45):34931-7
pubmed: 10944529
Biotechnol Biofuels. 2017 Aug 24;10:204
pubmed: 28855965
Biotechnol Biofuels. 2020 Feb 26;13:28
pubmed: 32127915
Biotechnol Bioeng. 2015 Nov;112(11):2228-40
pubmed: 25943255
Sci Rep. 2017 Sep 18;7(1):11739
pubmed: 28924169