Living cells as a biological analog of optical tweezers - a non-invasive microrheology approach.

Optical Tweezers Cytoskeleton / metabolism Cell Membrane / metabolism Elastic Modulus Actin Cytoskeleton
Cellular biomechanics Cytoskeleton Optical tweezers Passive microrheology

Journal

Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144

Informations de publication

Date de publication:
08 2023
Historique:
received: 22 12 2022
revised: 14 04 2023
accepted: 25 04 2023
medline: 3 7 2023
pubmed: 4 5 2023
entrez: 3 5 2023
Statut: ppublish

Résumé

Microrheology, the study of fluids on micron length-scales, promises to reveal insights into cellular biology, including mechanical biomarkers of disease and the interplay between biomechanics and cellular function. Here a minimally-invasive passive microrheology technique is applied to individual living cells by chemically binding a bead to the surface of a cell, and observing the mean squared displacement of the bead at timescales ranging from milliseconds to 100s of seconds. Measurements are repeated over the course of hours, and presented alongside analysis to quantify changes in the cells' low-frequency elastic modulus, G

Identifiants

DOI: 10.1016/j.actbio.2023.04.039 PMID: 37137402
pubmed: 37137402
pii: S1742-7061(23)00239-8
doi: 10.1016/j.actbio.2023.04.039
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

317-325

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

William Hardiman (W)

Optics and Photonics Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK. Electronic address: will.hardiman@nottingham.ac.uk.

Matt Clark (M)

Optics and Photonics Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK.

Claire Friel (C)

School of Life Sciences, University of Nottingham, Medical School, QMC, Nottingham NG7 2UH, UK.

Alan Huett (A)

School of Life Sciences, University of Nottingham, Medical School, QMC, Nottingham NG7 2UH, UK.

Fernando Pérez-Cota (F)

Optics and Photonics Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK.

Kerry Setchfield (K)

Optics and Photonics Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK.

Amanda J Wright (AJ)

Optics and Photonics Research Group, Faculty of Engineering, University of Nottingham, Nottingham NG7 2RD, UK. Electronic address: Amanda.Wright@Nottingham.ac.uk.

Manlio Tassieri (M)

Division of Biomedical Engineering, James Watt School of Engineering, University of Glasgow, Glasgow, G12 8LT, UK. Electronic address: Manlio.Tassieri@Glasgow.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Pinces optiques › Living cells as a biological analog of optical...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Living cells as a biological analog of optical...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Living cells as a biological analog of optical...
questionsmedicales.fr › Phénomènes physiques › Phénomènes mécaniques › Élasticité › Module d'élasticité › Living cells as a biological analog of optical...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Cytosquelette d'actine › Living cells as a biological analog of optical...