Biochemical and genotyping analyses of camels (Camelus dromedaries) trypanosomiasis in North Africa.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 05 2023
03 05 2023
Historique:
received:
30
11
2022
accepted:
24
04
2023
medline:
5
5
2023
pubmed:
4
5
2023
entrez:
3
5
2023
Statut:
epublish
Résumé
Camels are considered an important food source in North Africa. Trypanosomiasis in camels is a life-threatening disease that causes severe economic losses in milk and meat production. Therefore, the objective of this study was to determine the trypanosome genotypes in the North African region. Trypanosome infection rates were determined by microscopic examination of blood smears and polymerase chain reaction (PCR). In addition, total antioxidant capacity (TAC), lipid peroxides (MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were determined in erythrocyte lysate. Furthermore, 18S amplicon sequencing was used to barcode and characterizes the genetic diversity of trypanosome genotypes in camel blood. In addition to Trypanosoma, Babesia and Thelieria were also detected in the blood samples. PCR showed that the trypanosome infection rate was higher in Algerian samples (25.7%) than in Egyptian samples (7.2%). Parameters such as MDA, GSH, SOD and CAT had significantly increased in camels infected with trypanosomes compared to uninfected control animals, while TAC level was not significantly changed. The results of relative amplicon abundance showed that the range of trypanosome infection was higher in Egypt than in Algeria. Moreover, phylogenetic analysis showed that the Trypanosoma sequences of Egyptian and Algerian camels are related to Trypanosoma evansi. Unexpectedly, diversity within T. evansi was higher in Egyptian camels than in Algerian camels. We present here the first molecular report providing a picture of trypanosomiasis in camels, covering wide geographical areas in Egypt and Algeria.
Identifiants
pubmed: 37137920
doi: 10.1038/s41598-023-34086-y
pii: 10.1038/s41598-023-34086-y
pmc: PMC10156784
doi:
Substances chimiques
Antioxidants
0
Superoxide Dismutase
EC 1.15.1.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7176Informations de copyright
© 2023. The Author(s).
Références
Vet Parasitol. 2001 Jun 12;97(3):183-9
pubmed: 11390070
Genome Biol Evol. 2017 Aug 1;9(8):1990-1997
pubmed: 28541535
Infect Genet Evol. 2010 May;10(4):453-8
pubmed: 20302972
Vet Parasitol. 2009 Oct 28;165(1-2):41-6
pubmed: 19628334
Mol Biol Evol. 2020 May 1;37(5):1530-1534
pubmed: 32011700
BMC Genomics. 2012 Jul 24;13:341
pubmed: 22827831
Trends Genet. 2014 Sep;30(9):418-26
pubmed: 25108476
Trends Parasitol. 2022 Sep;38(9):724-736
pubmed: 35680542
Nature. 2003 Feb 27;421(6926):936-9
pubmed: 12606999
Vet Parasitol. 2011 Aug 25;180(3-4):349-53
pubmed: 21514732
Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1999-2004
pubmed: 18245376
Exp Parasitol. 2012 Aug;131(4):404-12
pubmed: 22659228
Genome Biol. 2010;11(5):210
pubmed: 20441597
Trends Parasitol. 2015 Nov;31(11):553-562
pubmed: 26463826
Clin Chim Acta. 2008 Apr;390(1-2):1-11
pubmed: 18243141
PLoS Negl Trop Dis. 2015 Jan 08;9(1):e3404
pubmed: 25568942
Trends Parasitol. 2001 Jan;17(1):19-24
pubmed: 11137736
PLoS One. 2013;8(1):e54835
pubmed: 23382983
Arch Biochem Biophys. 1959 May;82(1):70-7
pubmed: 13650640
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
Ecohealth. 2017 Mar;14(Suppl 1):128-138
pubmed: 28213652
Adv Parasitol. 2017;97:47-109
pubmed: 28325373
Vet Parasitol. 2015 Sep 15;212(3-4):118-22
pubmed: 26242833
Infect Genet Evol. 2002 May;1(3):207-14
pubmed: 12798017
Bioinformatics. 2009 Dec 15;25(24):3244-50
pubmed: 19819885
Nature. 1986 Jul 10-16;322(6075):173-5
pubmed: 3724860
Biochem Biophys Res Commun. 1972 Jan 31;46(2):849-54
pubmed: 4400444
Nat Biotechnol. 1996 Jun;14(6):717-21
pubmed: 9630977
Exp Parasitol. 2010 Jul;125(3):196-201
pubmed: 20109454
Kinetoplastid Biol Dis. 2004 Sep 17;3(1):3
pubmed: 15377385
J Clin Microbiol. 2017 Jul;55(7):2009-2017
pubmed: 28404673
Anal Biochem. 1979 Jun;95(2):351-8
pubmed: 36810
Vet Parasitol. 2007 Jun 20;147(1-2):51-60
pubmed: 17452081
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
Bioinformatics. 2019 Nov 1;35(21):4453-4455
pubmed: 31070718
Clin Chem. 1995 Dec;41(12 Pt 2):1819-28
pubmed: 7497639
PLoS Negl Trop Dis. 2011 Feb 08;5(2):e961
pubmed: 21347445
Parasitol Res. 2005 Feb;95(3):186-92
pubmed: 15619129
Vet Parasitol. 2009 Jun 10;162(3-4):192-9
pubmed: 19375230
Bioinformatics. 2009 Aug 1;25(15):1972-3
pubmed: 19505945
Acta Parasitol. 2018 Dec 19;63(4):733-743
pubmed: 30367776
J Clin Pathol. 2001 May;54(5):356-61
pubmed: 11328833
PLoS One. 2017 Jul 13;12(7):e0181279
pubmed: 28704541