Easy and Rapid Methods for Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Human Umbilical Wharton's Jelly-Derived Mesenchymal Stem Cells.
Explant method
Ficoll-Paque density gradient
Mesenchymal stem cell
Umbilical cord blood
Wharton’s jelly
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
medline:
8
11
2023
pubmed:
4
5
2023
entrez:
4
5
2023
Statut:
ppublish
Résumé
These protocols describe modified methods that use Ficoll-Paque density gradient for umbilical cord blood-derived mesenchymal stem cells and explant method for Wharton's jelly-derived mesenchymal stem cells. The Ficoll-Paque density gradient method allows to obtain mesenchymal stem cells while eliminating monocytic cells. In this method, precoating the cell culture flasks with fetal bovine serum helps remove the monocytic cells and instruct more pure mesenchymal stem cells. On the other hand, the explant method for Wharton's jelly-derived mesenchymal stem cell is user-friendly and cost-effective than enzymatic methods. In this chapter, we provide a collection of protocols to obtain mesenchymal stem cells from human umbilical cord blood and Wharton's jelly.
Identifiants
pubmed: 37140810
doi: 10.1007/7651_2023_479
doi:
Substances chimiques
Ficoll
25702-74-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
77-84Informations de copyright
© 2023. Springer Science+Business Media, LLC.
Références
Friedenstein AJ, Deriglasova UF, Kulagina NN et al (1974) Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol 2(2):83–92
pubmed: 4455512
Markov A, Thangavelu L, Aravindhan S et al (2021) Mesenchymal stem/stromal cells as a valuable source for the treatment of immune-mediated disorders. Stem Cell Res Ther 12(1):192
doi: 10.1186/s13287-021-02265-1
pubmed: 33736695
pmcid: 7971361
Zhu R, Yan T, Feng Y et al (2021) Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms. Cell Res 31(12):1244–1262
doi: 10.1038/s41422-021-00573-y
pubmed: 34702946
pmcid: 8546390
Lanzoni G, Linetsky E, Correa D et al (2021) Umbilical cord mesenchymal stem cells for COVID-19 acute respiratory distress syndrome: a double-blind, phase 1/2a, randomized controlled trial. Stem Cells Transl Med 10(5):660–673
doi: 10.1002/sctm.20-0472
pubmed: 33400390
pmcid: 8046040
Xu F, Fei Z, Dai H et al (2022) Mesenchymal stem cell-derived extracellular vesicles with high PD-L1 expression for autoimmune diseases treatment. Adv Mater 34(1):e2106265
doi: 10.1002/adma.202106265
pubmed: 34613627
Riazifar M, Mohammadi MR, Pone EJ et al (2019) Stem cell-derived exosomes as nanotherapeutics for autoimmune and neurodegenerative disorders. ACS Nano 13(6):6670–6688
doi: 10.1021/acsnano.9b01004
pubmed: 31117376
pmcid: 6880946
Rautiainen S, Laaksonen T, Koivuniemi R (2021) Angiogenic effects and crosstalk of adipose-derived mesenchymal stem/stromal cells and their extracellular vesicles with endothelial cells. Int J Mol Sci 22(19):10890
doi: 10.3390/ijms221910890
pubmed: 34639228
pmcid: 8509224
Aydin S, Şahin F (2019) Stem cells derived from dental tissues. Adv Exp Med Biol 1144:123–132
doi: 10.1007/5584_2018_333
pubmed: 30635857
Kim HJ, Cho KR, Jang H et al (2021) Intracerebroventricular injection of human umbilical cord blood mesenchymal stem cells in patients with Alzheimer’s disease dementia: a phase I clinical trial. Alzheimers Res Ther 13(1):154
doi: 10.1186/s13195-021-00897-2
pubmed: 34521461
pmcid: 8439008
Ranjbaran H, Abediankenari S, Mohammadi M et al (2018) Wharton’s jelly derived-mesenchymal stem cells: isolation and characterization. Acta Med Iran 56(1):28–33
pubmed: 29436792
Dominici M, Le Blanc K, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8(4):315–317
doi: 10.1080/14653240600855905
pubmed: 16923606
Atashi F, Modarressi A, Pepper MS (2015) The role of reactive oxygen species in mesenchymal stem cell adipogenic and osteogenic differentiation: a review. Stem Cells Dev 24(10):1150–1163
doi: 10.1089/scd.2014.0484
pubmed: 25603196
pmcid: 4424969
Li M, Yin H, Yan Z et al (2022) The immune microenvironment in cartilage injury and repair. Acta Biomater 140:23–42
doi: 10.1016/j.actbio.2021.12.006
pubmed: 34896634
Chen Q, Shou P, Zheng C et al (2016) Fate decision of mesenchymal stem cells: adipocytes or osteoblasts? Cell Death Differ 23(7):1128–1139
doi: 10.1038/cdd.2015.168
pubmed: 26868907
pmcid: 4946886
Chen Y, Shen H, Ding Y et al (2021) The application of umbilical cord-derived MSCs in cardiovascular diseases. J Cell Mol Med 25(17):8103–8114
doi: 10.1111/jcmm.16830
pubmed: 34378345
pmcid: 8419197
Marrelli M, Paduano F, Tatullo M (2015) Human periapical cyst-mesenchymal stem cells differentiate into neuronal cells. J Dent Res 94(6):843–852
doi: 10.1177/0022034515570316
pubmed: 25672890
Samaeekia R, Rabiee B, Putra I et al (2018) Effect of human corneal mesenchymal stromal cell-derived exosomes on corneal epithelial wound healing. Invest Ophthalmol Vis Sci 59(12):5194–5200
doi: 10.1167/iovs.18-24803
pubmed: 30372747
pmcid: 6203220
Cruz FF, Rocco PRM (2020) The potential of mesenchymal stem cell therapy for chronic lung disease. Expert Rev Respir Med 14(1):31–39
doi: 10.1080/17476348.2020.1679628
pubmed: 31608724
Marofi F, Alexandrovna KI, Margiana R et al (2021) MSCs and their exosomes: a rapidly evolving approach in the context of cutaneous wounds therapy. Stem Cell Res Ther 12(1):597
doi: 10.1186/s13287-021-02662-6
pubmed: 34863308
pmcid: 8642895
Dong L, Wang Y, Zheng T et al (2021) Hypoxic hUCMSC-derived extracellular vesicles attenuate allergic airway inflammation and airway remodeling in chronic asthma mice. Stem Cell Res Ther 12(1):4
doi: 10.1186/s13287-020-02072-0
pubmed: 33407872
pmcid: 7789736
Hmadcha A, Martin-Montalvo A, Gauthier BR, Soria B, Capilla-Gonzalez V (2020) Therapeutic potential of mesenchymal stem cells for cancer therapy. Front Bioeng Biotechnol 8:43
doi: 10.3389/fbioe.2020.00043
pubmed: 32117924
pmcid: 7013101
Niess H, Thomas MN, Schiergens TS et al (2016) Genetic engineering of mesenchymal stromal cells for cancer therapy: turning partners in crime into Trojan horses. Innov Surg Sci 1(1):19–32
pubmed: 31579715
pmcid: 6753982
Zhu Y, Sun Z, Han Q et al (2009) Human mesenchymal stem cells inhibit cancer cell proliferation by secreting DKK-1. Leukemia 23(5):925–933
doi: 10.1038/leu.2008.384
pubmed: 19148141
Vianello F, Villanova F, Tisato V et al (2010) Bone marrow mesenchymal stromal cells non-selectively protect chronic myeloid leukemia cells from imatinib-induced apoptosis via the CXCR4/CXCL12 axis. Haematologica 95(7):1081–1089
doi: 10.3324/haematol.2009.017178
pubmed: 20179085
pmcid: 2895031
Yu K, Yin Y, Ma D et al (2020) Shp2 activation in bone marrow microenvironment mediates the drug resistance of B-cell acute lymphoblastic leukemia through enhancing the role of VCAM-1/VLA-4. Int Immunopharmacol 80:106008
doi: 10.1016/j.intimp.2019.106008
pubmed: 31978797
Sel FA, Oguz FS (2022) Regenerative medicine application of mesenchymal stem cells. In: Turksen K (ed) Cell Biology and Translational Medicine, Volume 16. Advances in Experimental Medicine and Biology, vol 1387. Springer, Cham