Inhibition of mouse trypsin isoforms by SPINK1 and effect of human pancreatitis-associated mutations.
Chronic pancreatitis
Inflammation
Inhibition
Mutation
Trypsin
Journal
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]
ISSN: 1424-3911
Titre abrégé: Pancreatology
Pays: Switzerland
ID NLM: 100966936
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
04
04
2023
revised:
26
04
2023
accepted:
28
04
2023
medline:
5
6
2023
pubmed:
7
5
2023
entrez:
6
5
2023
Statut:
ppublish
Résumé
Serine protease inhibitor Kazal type 1 (SPINK1) is a trypsin-selective inhibitor protein secreted by the exocrine pancreas. Loss-of-function SPINK1 mutations predispose to chronic pancreatitis through either reduced expression, secretion, or impaired trypsin inhibition. In this study, we aimed to characterize the inhibitory activity of mouse SPINK1 against cationic (T7) and anionic (T8, T9, T20) mouse trypsin isoforms. Kinetic measurements with a peptide substrate, and digestion experiments with β-casein indicated that the catalytic activity of all mouse trypsins is comparable. Human SPINK1 and its mouse ortholog inhibited mouse trypsins with comparable efficiency (K
Identifiants
pubmed: 37149461
pii: S1424-3903(23)00137-0
doi: 10.1016/j.pan.2023.04.043
pii:
doi:
Substances chimiques
Trypsin Inhibitor, Kazal Pancreatic
50936-63-5
Trypsin
EC 3.4.21.4
Protein Isoforms
0
SPINK1 protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
358-366Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.