Control of Enzyme Reactivity in Response to Osmotic Pressure Modulation Mimicking Dynamic Assembly of Intracellular Organelles.
artificial cells
elastin-like polypeptides
liquid-liquid phase separation
microfluidics
synthetic organelles
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
revised:
17
04
2023
received:
27
02
2023
medline:
18
8
2023
pubmed:
7
5
2023
entrez:
7
5
2023
Statut:
ppublish
Résumé
In response to variations in osmotic stress, in particular to hypertonicity associated with biological dysregulations, cells have developed complex mechanisms to release their excess water, thus avoiding their bursting and death. When water is expelled, cells shrink and concentrate their internal bio(macro)molecular content, inducing the formation of membraneless organelles following a liquid-liquid phase separation (LLPS) mechanism. To mimic this intrinsic property of cells, functional thermo-responsive elastin-like polypeptide (ELP) biomacromolecular conjugates are herein encapsulated into self-assembled lipid vesicles using a microfluidic system, together with polyethylene glycol (PEG) to mimic cells' interior crowded microenvironment. By inducing a hypertonic shock onto the vesicles, expelled water induces a local increase in concentration and a concomitant decrease in the cloud point temperature (T
Identifiants
pubmed: 37149761
doi: 10.1002/adma.202301856
doi:
Substances chimiques
Peptides
0
Horseradish Peroxidase
EC 1.11.1.-
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2301856Subventions
Organisme : IdEx Bordeaux
ID : 10-IDEX-03-02
Organisme : Marie Skłodowska - Curie
ID : BIOMOLMACS
Organisme : Marie Skłodowska - Curie
ID : 859416
Informations de copyright
© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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