Direct observation of epoxy resin blocks for renal biopsy by low-vacuum scanning electron microscopy.
Electron microscopy
Glomerulus
LVSEM
Podocyte
Renal biopsy
Journal
Medical molecular morphology
ISSN: 1860-1499
Titre abrégé: Med Mol Morphol
Pays: Japan
ID NLM: 101239023
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
20
02
2023
accepted:
26
04
2023
medline:
28
11
2023
pubmed:
11
5
2023
entrez:
10
5
2023
Statut:
ppublish
Résumé
To improve the resolution of low-vacuum scanning electron microscopy (LVSEM), the epoxy resin block for the transmission electron microscopy (TEM) was observed directly with LVSEM. After observing ultrathin sections from renal biopsies of IgA nephropathy, membranous nephropathy, lupus nephritis, diabetic nephropathy (DM), thin basement membrane disease (TBMD), Alport's syndrome, Fabry's disease, and renal amyloidosis, the epoxy resin blocks of the same sites were observed by LVSEM and compared. The LVSEM image of the epoxy resin block corresponds to the negative of the TEM image, and when the gradation is reversed, the LVSEM image was comparable to the TEM image. At a low magnification of 100 ×, the entire specimen, including the glomerulus, was obtained. LVSEM at 5000 × magnification was sufficient to identify paramesangial deposits in IgA nephropathy and subepithelial electron-dense deposits (EDD) and spikes in membranous nephropathy. Glomerular basement membrane thickening in DM and thinning in TBMD could be sufficiently diagnosed with LVSEM at 6000 ×. Accumulation of ceramide in Fabry's disease was easily identified, but amyloid fibril could not be identified by LVSEM. LVSEM of renal biopsy epoxy resin blocks can replace TEM up to moderate magnification.
Identifiants
pubmed: 37165248
doi: 10.1007/s00795-023-00356-x
pii: 10.1007/s00795-023-00356-x
pmc: PMC10415507
doi:
Substances chimiques
Epoxy Resins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
206-216Subventions
Organisme : Dr. Naohiko Kobayashi research donation
ID : #2022-9
Informations de copyright
© 2023. The Author(s).
Références
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