Organic Electronic Platform for Real-Time Phenotypic Screening of Extracellular-Vesicle-Driven Breast Cancer Metastasis.


Journal

Advanced healthcare materials
ISSN: 2192-2659
Titre abrégé: Adv Healthc Mater
Pays: Germany
ID NLM: 101581613

Informations de publication

Date de publication:
10 2023
Historique:
revised: 21 04 2023
received: 15 04 2023
medline: 30 10 2023
pubmed: 12 5 2023
entrez: 12 5 2023
Statut: ppublish

Résumé

Tumor-derived extracellular vesicles (TEVs) induce the epithelial-to-mesenchymal transition (EMT) in nonmalignant cells to promote invasion and cancer metastasis, representing a novel therapeutic target in a field severely lacking in efficacious antimetastasis treatments. However, scalable technologies that allow continuous, multiparametric monitoring for identifying metastasis inhibitors are absent. Here, the development of a functional phenotypic screening platform based on organic electrochemical transistors (OECTs) for real-time, noninvasive monitoring of TEV-induced EMT and screening of antimetastatic drugs is reported. TEVs derived from the triple-negative breast cancer cell line MDA-MB-231 induce EMT in nonmalignant breast epithelial cells (MCF10A) over a nine-day period, recapitulating a model of invasive ductal carcinoma metastasis. Immunoblot analysis and immunofluorescence imaging confirm the EMT status of TEV-treated cells, while dual optical and electrical readouts of cell phenotype are obtained using OECTs. Further, heparin, a competitive inhibitor of cell surface receptors, is identified as an effective blocker of TEV-induced EMT. Together, these results demonstrate the utility of the platform for TEV-targeted drug discovery, allowing for facile modeling of the transient drug response using electrical measurements, and provide proof of concept that inhibitors of TEV function have potential as antimetastatic drug candidates.

Identifiants

pubmed: 37171457
doi: 10.1002/adhm.202301194
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2301194

Informations de copyright

© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.

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Auteurs

Walther C Traberg (WC)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.

Johana Uribe (J)

Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, Olin Hall, Ithaca, NY, 14853, USA.

Victor Druet (V)

Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 3955, Kingdom of Saudi Arabia.

Adel Hama (A)

Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 3955, Kingdom of Saudi Arabia.

Chrysanthi-Maria Moysidou (CM)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.

Miriam Huerta (M)

Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, Olin Hall, Ithaca, NY, 14853, USA.

Reece McCoy (R)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.

Daniel Hayward (D)

Early Cancer Institute, University of Cambridge, Hutchison Research Centre, Cambridge, CB2 0XZ, UK.

Achilleas Savva (A)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.

Amaury M R Genovese (AMR)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.

Suraj Pavagada (S)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.
Early Cancer Institute, University of Cambridge, Hutchison Research Centre, Cambridge, CB2 0XZ, UK.

Zixuan Lu (Z)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.

Anil Koklu (A)

Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 3955, Kingdom of Saudi Arabia.

Anna-Maria Pappa (AM)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.
Healthcare Innovation Engineering Center, Khalifa University, Abu Dhabi, PO Box 127788, United Arab Emirates.
Department of Biomedical Engineering, Khalifa University of Science and Technology, Abu Dhabi, PO Box 127788, United Arab Emirates.

Rebecca Fitzgerald (R)

Early Cancer Institute, University of Cambridge, Hutchison Research Centre, Cambridge, CB2 0XZ, UK.

Sahika Inal (S)

Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 3955, Kingdom of Saudi Arabia.

Susan Daniel (S)

Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell University, Olin Hall, Ithaca, NY, 14853, USA.

Róisín M Owens (RM)

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, CB3 0AS, UK.

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