Combined Structural and Computational Study of the mRubyFT Fluorescent Timer Locked in Its Blue Form.
blue chromophore
cell timer
crystal structure
fluorescent protein
fluorescent timer
mRuby
mRubyFT
molecular modeling
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
26 Apr 2023
26 Apr 2023
Historique:
received:
30
03
2023
revised:
21
04
2023
accepted:
25
04
2023
medline:
15
5
2023
pubmed:
13
5
2023
entrez:
13
5
2023
Statut:
epublish
Résumé
The mRubyFT is a monomeric genetically encoded fluorescent timer based on the mRuby2 fluorescent protein, which is characterized by the complete maturation of the blue form with the subsequent conversion to the red one. It has higher brightness in mammalian cells and higher photostability compared with other fluorescent timers. A high-resolution structure is a known characteristic of the mRubyFT with the red form chromophore, but structural details of its blue form remain obscure. In order to obtain insight into this, we obtained an S148I variant of the mRubyFT (mRubyFT
Identifiants
pubmed: 37175610
pii: ijms24097906
doi: 10.3390/ijms24097906
pmc: PMC10178504
pii:
doi:
Substances chimiques
Luminescent Proteins
0
Coloring Agents
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Russian Science Foundation
ID : 21-74-20135
Organisme : Russian Science Foundation
ID : 22-13-00012
Références
J Chem Inf Model. 2021 Oct 25;61(10):5125-5132
pubmed: 34601882
J Chem Phys. 2015 Feb 21;142(7):074111
pubmed: 25702006
J Chem Inf Model. 2020 Dec 28;60(12):6288-6297
pubmed: 33206518
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
J Biol Chem. 2010 May 21;285(21):15978-84
pubmed: 20220148
Acc Chem Res. 2012 Feb 21;45(2):265-75
pubmed: 21882809
Curr Opin Struct Biol. 2012 Oct;22(5):679-88
pubmed: 23000031
J Chem Theory Comput. 2012 Sep 11;8(9):3257-3273
pubmed: 23341755
Science. 2016 Sep 30;353(6307):1536-1541
pubmed: 27708103
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12501-4
pubmed: 7809066
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2256-68
pubmed: 15572779
Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):760-3
pubmed: 15299374
J Phys Chem B. 2010 Jul 29;114(29):9698-705
pubmed: 20593847
Nat Chem Biol. 2009 Feb;5(2):118-26
pubmed: 19136976
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82
pubmed: 16369096
J Comput Chem. 2011 Jul 15;32(9):1929-43
pubmed: 21469161
Int J Mol Sci. 2022 Mar 16;23(6):
pubmed: 35328628
J Comput Chem. 2010 Mar;31(4):671-90
pubmed: 19575467
Chem Rev. 2017 Jan 25;117(2):758-795
pubmed: 27754659
Int J Mol Sci. 2023 Feb 07;24(4):
pubmed: 36834686
PLoS One. 2011;6(12):e28674
pubmed: 22174863
Nature. 1995 Feb 23;373(6516):663-4
pubmed: 7854443
J Am Chem Soc. 2006 Mar 15;128(10):3166-8
pubmed: 16522096
J Chem Phys. 2020 Jul 28;153(4):044130
pubmed: 32752662
J Chem Phys. 2010 Apr 21;132(15):154104
pubmed: 20423165
Nat Methods. 2018 May;15(5):351-354
pubmed: 29578535
J Comput Chem. 2012 Feb 15;33(5):580-92
pubmed: 22162017
J Am Chem Soc. 2010 Feb 24;132(7):2243-53
pubmed: 20121102
Nat Biotechnol. 2006 Dec;24(12):1569-71
pubmed: 17115054
J Fluoresc. 2020 Dec;30(6):1287-1293
pubmed: 32833117
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Front Mol Neurosci. 2013 Mar 04;6:2
pubmed: 23459413
J Phys Chem A. 2013 Apr 11;117(14):3100-8
pubmed: 23514314
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):368-80
pubmed: 22505257
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Chem Biol. 2004 Jun;11(6):845-54
pubmed: 15217617
Biochemistry. 2007 Mar 27;46(12):3775-83
pubmed: 17323929
Chem Biol. 2010 Apr 23;17(4):333-41
pubmed: 20416505
Acta Crystallogr D Struct Biol. 2018 Feb 1;74(Pt 2):85-97
pubmed: 29533234