Lawsone encapsulated polylactic-co-glycolic acid nanoparticles modified with chitosan-folic acid successfully inhibited cell growth and triggered apoptosis in Panc-1 cancer cells.
drug delivery systems
nanobiotechnology
nanomedicine
nanotechnology
polymer structure
Journal
IET nanobiotechnology
ISSN: 1751-875X
Titre abrégé: IET Nanobiotechnol
Pays: United States
ID NLM: 101303205
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
revised:
12
04
2023
received:
18
01
2023
accepted:
29
04
2023
medline:
31
7
2023
pubmed:
16
5
2023
entrez:
16
5
2023
Statut:
ppublish
Résumé
The present research aims to encapsulate lawsone in polylactic-co-glycolic acid (PLGA) nanoparticles modified with folic acid (FA) and chitosan (CS) to study its anticancer effects against Panc-1 cells. The nanoparticles were analysed in means of shape/size and zeta potential index using scanning electron microscope and dynamic light scattering. High-performance liquid chromatography was applied to evaluate the lawsone entrapment efficacy. The authors performed acridine orange/propidium iodide staining and flow cytometry to monitor apoptosis induction and cell cycle arrest. The expressions of apoptosis-related genes (BAX and BCL-2) were assessed by real time PCR. Nanoparticle antioxidative and antibacterial activities were examined by DPPH/ABTS scavenging assay, disk diffusion method, and minimum inhibitory concentration and minimum bactericidal concentration evaluation. The NPs were 229.65 nm, the encapsulation efficiency was 81%. The concentration of lawsone that exerts 50% cell growth inhibition (IC
Identifiants
pubmed: 37191102
doi: 10.1049/nbt2.12139
pmc: PMC10374556
doi:
Substances chimiques
Polylactic Acid-Polyglycolic Acid Copolymer
1SIA8062RS
Chitosan
9012-76-4
lawsone
TLH4A6LV1W
Polyglycolic Acid
26009-03-0
Folic Acid
935E97BOY8
Lactic Acid
33X04XA5AT
Glycols
0
glycolic acid
0WT12SX38S
bcl-2-Associated X Protein
0
Drug Carriers
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
425-437Informations de copyright
© 2023 The Authors. IET Nanobiotechnology published by John Wiley & Sons Ltd.
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