Collagen Protein Ingestion during Recovery from Exercise Does Not Increase Muscle Connective Protein Synthesis Rates.
Journal
Medicine and science in sports and exercise
ISSN: 1530-0315
Titre abrégé: Med Sci Sports Exerc
Pays: United States
ID NLM: 8005433
Informations de publication
Date de publication:
01 10 2023
01 10 2023
Historique:
medline:
18
9
2023
pubmed:
19
5
2023
entrez:
19
5
2023
Statut:
ppublish
Résumé
Protein ingestion during recovery from exercise has been reported to augment myofibrillar protein synthesis rates, without increasing muscle connective protein synthesis rates. It has been suggested that collagen protein may be effective in stimulating muscle connective protein synthesis. The present study assessed the capacity of both whey and collagen protein ingestion to stimulate postexercise myofibrillar and muscle connective protein synthesis rates. In a randomized, double-blind, parallel design, 45 young male ( n = 30) and female ( n = 15) recreational athletes (age, 25 ± 4 yr; body mass index, 24.1 ± 2.0 kg·m -2 ) were selected to receive primed continuous intravenous infusions with l -[ring- 13 C 6 ]-phenylalanine and l -[3,5- 2 H 2 ]-tyrosine. After a single session of resistance type exercise, subjects were randomly allocated to one of three groups ingesting either 30 g whey protein (WHEY, n = 15), 30 g collagen protein (COLL, n = 15) or a noncaloric placebo (PLA, n = 15). Blood and muscle biopsy samples were collected over a subsequent 5-h recovery period to assess both myofibrillar and muscle connective protein synthesis rates. Protein ingestion increased circulating plasma amino acid concentrations ( P < 0.05). The postprandial rise in plasma leucine and essential amino acid concentrations was greater in WHEY compared with COLL, whereas plasma glycine and proline concentrations increased more in COLL compared with WHEY ( P < 0.05). Myofibrillar protein synthesis rates averaged 0.041 ± 0.010, 0.036 ± 0.010, and 0.032 ± 0.007%·h -1 in WHEY, COLL and PLA, respectively, with only WHEY resulting in higher rates when compared with PLA ( P < 0.05). Muscle connective protein synthesis rates averaged 0.072 ± 0.019, 0.068 ± 0.017, and 0.058 ± 0.018%·h -1 in WHEY, COLL, and PLA, respectively, with no significant differences between groups ( P = 0.09). Ingestion of whey protein during recovery from exercise increases myofibrillar protein synthesis rates. Neither collagen nor whey protein ingestion further increased muscle connective protein synthesis rates during the early stages of postexercise recovery in both male and female recreational athletes.
Identifiants
pubmed: 37202878
doi: 10.1249/MSS.0000000000003214
pii: 00005768-202310000-00008
pmc: PMC10487367
doi:
Substances chimiques
Muscle Proteins
0
Whey Proteins
0
Collagen
9007-34-5
Polyesters
0
Dietary Proteins
0
Types de publication
Randomized Controlled Trial
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1792-1802Informations de copyright
Copyright © 2023 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American College of Sports Medicine.
Références
Biolo G, Maggi SP, Williams BD, Tipton KD, Wolfe RR. Increased rates of muscle protein turnover and amino acid transport after resistance exercise in humans. Am J Phys . 1995;268(3 Pt 1):E514–20.
Phillips SM, Tipton KD, Aarsland A, Wolf SE, Wolfe RR. Mixed muscle protein synthesis and breakdown after resistance exercise in humans. Am J Phys . 1997;273(1 Pt 1):E99–107.
Pennings B, Koopman R, Beelen M, Senden JM, Saris WH, van Loon LJ. Exercising before protein intake allows for greater use of dietary protein–derived amino acids for de novo muscle protein synthesis in both young and elderly men. Am J Clin Nutr . 2010;93(2):322–31.
Beelen M, Koopman R, Gijsen AP, et al. Protein coingestion stimulates muscle protein synthesis during resistance-type exercise. Am J Physiol Endocrinol Metab . 2008;295(1):E70–7.
Holwerda AM, Paulussen KJM, Overkamp M, et al. Dose-dependent increases in whole-body net protein balance and dietary protein-derived amino acid incorporation into myofibrillar protein during recovery from resistance exercise in older men. J Nutr . 2019;149(2):221–30.
Moore DR, Robinson MJ, Fry JL, et al. Ingested protein dose response of muscle and albumin protein synthesis after resistance exercise in young men. Am J Clin Nutr . 2009;89(1):161–8.
Wilkinson SB, Tarnopolsky MA, Macdonald MJ, Macdonald JR, Armstrong D, Phillips SM. Consumption of fluid skim milk promotes greater muscle protein accretion after resistance exercise than does consumption of an isonitrogenous and isoenergetic soy-protein beverage. Am J Clin Nutr . 2007;85(4):1031–40.
Tang JE, Moore DR, Kujbida GW, Tarnopolsky MA, Phillips SM. Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men. J Appl Physiol (1985) . 2009;107(3):987–92.
Witard OC, Jackman SR, Breen L, Smith K, Selby A, Tipton KD. Myofibrillar muscle protein synthesis rates subsequent to a meal in response to increasing doses of whey protein at rest and after resistance exercise. Am J Clin Nutr . 2014;99(1):86–95.
Huijing PA. Muscle as a collagen fiber reinforced composite: a review of force transmission in muscle and whole limb. J Biomech . 1999;32(4):329–45.
Gillies AR, Lieber RL. Structure and function of the skeletal muscle extracellular matrix. Muscle Nerve . 2011;44(3):318–31.
Zhang C, Gao Y. Effects of aging on the lateral transmission of force in rat skeletal muscle. J Biomech . 2014;47(5):944–8.
Holm L, Van Hall G, Rose AJ, et al. Contraction intensity and feeding affect collagen and myofibrillar protein synthesis rates differently in human skeletal muscle. Am J Physiol Endocrinol Metab . 2010;298(2):E257–69.
Miller BF, Olesen JL, Hansen M, et al. Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise. J Physiol . 2005;567(3):1021–33.
Moore DR, Phillips SM, Babraj JA, Smith K, Rennie MJ. Myofibrillar and collagen protein synthesis in human skeletal muscle in young men after maximal shortening and lengthening contractions. Am J Physiol Endocrinol Metab . 2005;288(6):E1153–9.
Trommelen J, Holwerda AM, Senden JM, et al. Casein ingestion does not increase muscle connective tissue protein synthesis rates. Med Sci Sports Exerc . 2020;52(9):1983–91.
Holwerda AM, Trommelen J, Kouw IWK, et al. Exercise plus presleep protein ingestion increases overnight muscle connective tissue protein synthesis rates in healthy older men. Int J Sport Nutr Exerc Metab . 2021;31(3):217–26.
Cuthbertson DJ, Babraj J, Smith K, et al. Anabolic signaling and protein synthesis in human skeletal muscle after dynamic shortening or lengthening exercise. Am J Physiol Endocrinol Metab . 2006;290(4):E731–8.
Oikawa SY, Kamal MJ, Webb EK, McGlory C, Baker SK, Phillips SM. Whey protein but not collagen peptides stimulate acute and longer-term muscle protein synthesis with and without resistance exercise in healthy older women: a randomized controlled trial. Am J Clin Nutr . 2020;111(3):708–18.
Dideriksen KJ, Reitelseder S, Petersen SG, et al. Stimulation of muscle protein synthesis by whey and caseinate ingestion after resistance exercise in elderly individuals. Scand J Med Sci Sports . 2011;21(6):e372–83.
Dideriksen K, Reitelseder S, Malmgaard-Clausen NM, et al. No effect of anti-inflammatory medication on postprandial and postexercise muscle protein synthesis in elderly men with slightly elevated systemic inflammation. Exp Gerontol . 2016;83:120–9.
Babraj JA, Cuthbertson DJ, Smith K, et al. Collagen synthesis in human musculoskeletal tissues and skin. Am J Physiol Endocrinol Metab . 2005;289(5):E864–9.
Mikkelsen UR, Dideriksen K, Andersen MB, et al. Preserved skeletal muscle protein anabolic response to acute exercise and protein intake in well-treated rheumatoid arthritis patients. Arthritis Res Ther . 2015;17:271.
Holm L, Rahbek SK, Farup J, Vendelbo MH, Vissing K. Contraction mode and whey protein intake affect the synthesis rate of intramuscular connective tissue. Muscle Nerve . 2017;55(1):128–30.
Alcock RD, Shaw GC, Tee N, Burke LM. Plasma amino acid concentrations after the ingestion of dairy and collagen proteins, in healthy active males. Front Nutr . 2019;6:163.
Gorissen SHM, Crombag JJR, Senden JMG, et al. Protein content and amino acid composition of commercially available plant-based protein isolates. Amino Acids . 2018;50(12):1685–95.
Eastoe JE. The amino acid composition of mammalian collagen and gelatin. Biochem J . 1955;61(4):589–600.
Skov K, Oxfeldt M, Thøgersen R, Hansen M, Bertram HC. Enzymatic hydrolysis of a collagen hydrolysate enhances postprandial absorption rate—a randomized controlled trial. Nutrients . 2019;11(5):1064.
Shaw G, Lee-Barthel A, Ross ML, Wang B, Baar K. Vitamin C-enriched gelatin supplementation before intermittent activity augments collagen synthesis. Am J Clin Nutr . 2017;105(1):136–43.
Oikawa SY, McGlory C, D’Souza LK, et al. A randomized controlled trial of the impact of protein supplementation on leg lean mass and integrated muscle protein synthesis during inactivity and energy restriction in older persons. Am J Clin Nutr . 2018;108(5):1060–8.
Brzycki M. Strength testing—predicting a one-rep max from reps-to-fatigue. J Phys Educ Recreat Dance . 1993;64(1):88–90.
Vickers AJ. Comparison of an ordinal and a continuous outcome measure of muscle soreness. Int J Technol Assess Health Care . 1999;15(4):709–16.
Vasikaran S, Eastell R, Bruyère O, et al. Markers of bone turnover for the prediction of fracture risk and monitoring of osteoporosis treatment: a need for international reference standards. Osteoporos Int . 2011;22(2):391–420.
Burd NA, Groen BB, Beelen M, Senden JM, Gijsen AP, Van Loon LJ. The reliability of using the single-biopsy approach to assess basal muscle protein synthesis rates in vivo in humans. Metabolism . 2012;61(7):931–6.
Burd NA, Pennings B, Groen BB, Gijsen AP, Senden JM, Van Loon LJ. The single biopsy approach is reliable for the measurement of muscle protein synthesis rates in vivo in older men. J Appl Physiol (1985) . 2012;113(6):896–902.
Monsen ER. Dietary reference intakes for the antioxidant nutrients: vitamin C, vitamin E, selenium, and carotenoids. J Am Diet Assoc . 2000;100(6):637–40.
Chesley A, MacDougall JD, Tarnopolsky MA, Atkinson SA, Smith K. Changes in human muscle protein synthesis after resistance exercise. J Appl Physiol (1985) . 1992;73(4):1383–8.
Pennings B, Boirie Y, Senden JM, Gijsen AP, Kuipers H, van Loon LJ. Whey protein stimulates postprandial muscle protein accretion more effectively than do casein and casein hydrolysate in older men. Am J Clin Nutr . 2011;93(5):997–1005.
Wilk KE, Escamilla RF, Fleisig GS, Barrentine SW, Andrews JR, Boyd ML. A comparison of tibiofemoral joint forces and electromyographic activit during open and closed kinetic chain exercises. Am J Sports Med . 1996;24(4):518–27.
Trommelen J, Betz MW, van Loon LJC. The muscle protein synthetic response to meal ingestion following resistance-type exercise. Sports Med . 2019;49(2):185–97.
Yang Y, Breen L, Burd NA, et al. Resistance exercise enhances myofibrillar protein synthesis with graded intakes of whey protein in older men. Br J Nutr . 2012;108(10):1780–8.
Volpi E, Kobayashi H, Sheffield-Moore M, Mittendorfer B, Wolfe RR. Essential amino acids are primarily responsible for the amino acid stimulation of muscle protein anabolism in healthy elderly adults. Am J Clin Nutr . 2003;78(2):250–8.
Devries MC, McGlory C, Bolster DR, et al. Protein leucine content is a determinant of shorter- and longer-term muscle protein synthetic responses at rest and following resistance exercise in healthy older women: a randomized, controlled trial. Am J Clin Nutr . 2018;107(2):217–26.
Moore DR, Tang JE, Burd NA, Rerecich T, Tarnopolsky MA, Phillips SM. Differential stimulation of myofibrillar and sarcoplasmic protein synthesis with protein ingestion at rest and after resistance exercise. J Physiol . 2009;587(Pt 4):897–904.
König D, Oesser S, Scharla S, Zdzieblik D, Gollhofer A. Specific collagen peptides improve bone mineral density and bone markers in postmenopausal women-a randomized controlled study. Nutrients . 2018;10(1):97.
Kirmse M, Oertzen-Hagemann V, de Marées M, Bloch W, Platen P. Prolonged collagen peptide supplementation and resistance exercise training affects body composition in recreationally active men. Nutrients . 2019;11(5):1154.
Dolan E, Varley I, Ackerman KE, Pereira RMR, Elliott-Sale KJ, Sale C. The bone metabolic response to exercise and nutrition. Exerc Sport Sci Rev . 2020;48(2):49–58.
Dolan E, Dumas A, Keane KM, et al. The bone biomarker response to an acute bout of exercise: a systematic review with meta-analysis. Sports Med . 2022;52(12):2889–908.
Qvist P, Christgau S, Pedersen BJ, Schlemmer A, Christiansen C. Circadian variation in the serum concentration of C-terminal telopeptide of type I collagen (serum CTx): effects of gender, age, menopausal status, posture, daylight, serum cortisol, and fasting. Bone . 2002;31(1):57–61.
Henriksen DB, Alexandersen P, Bjarnason NH, et al. Role of gastrointestinal hormones in postprandial reduction of bone resorption. J Bone Miner Res . 2003;18(12):2180–9.