Oocyte secreted factors control genes regulating FSH signaling and the maturation cascade in cumulus cells: the oocyte is not in a hurry.
Cumulus cells
EGF-like maturation cascade
FSH
Oocyte-secreted factors (OSFs)
mRNA
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
16
01
2023
accepted:
29
04
2023
pmc-release:
01
08
2024
medline:
27
7
2023
pubmed:
19
5
2023
entrez:
19
5
2023
Statut:
ppublish
Résumé
To assess the effects of the oocyte on mRNA abundance of FSHR, AMH and major genes of the maturation cascade (AREG, EREG, ADAM17, EGFR, PTGS2, TNFAIP6, PTX3, and HAS2) in bovine cumulus cells. (1) Intact cumulus-oocyte complexes, (2) microsurgically oocytectomized cumulus-oolema complexes (OOX), and (3) OOX + denuded oocytes (OOX+DO) were subjected to in vitro maturation (IVM) stimulated with FSH for 22 h or with AREG for 4 and 22 h. After IVM, cumulus cells were separated and relative mRNA abundance was measured by RT-qPCR. After 22 h of FSH-stimulated IVM, oocytectomy increased FSHR mRNA levels (p=0.005) while decreasing those of AMH (p=0.0004). In parallel, oocytectomy increased mRNA abundance of AREG, EREG, ADAM17, PTGS2, TNFAIP6, and PTX3, while decreasing that of HAS2 (p<0.02). All these effects were abrogated in OOX+DO. Oocytectomy also reduced EGFR mRNA levels (p=0.009), which was not reverted in OOX+DO. The stimulatory effect of oocytectomy on AREG mRNA abundance (p=0.01) and its neutralization in OOX+DO was again observed after 4 h of AREG-stimulated IVM. After 22 h of AREG-stimulated IVM, oocytectomy and addition of DOs to OOX caused the same effects on gene expression observed after 22 h of FSH-stimulated IVM, except for ADAM17 (p<0.025). These findings suggest that oocyte-secreted factors inhibit FSH signaling and the expression of major genes of the maturation cascade in cumulus cells. These may be important actions of the oocyte favoring its communication with cumulus cells and preventing premature activation of the maturation cascade.
Identifiants
pubmed: 37204638
doi: 10.1007/s10815-023-02822-y
pii: 10.1007/s10815-023-02822-y
pmc: PMC10371970
doi:
Substances chimiques
Epidermal Growth Factor
62229-50-9
Cyclooxygenase 2
EC 1.14.99.1
Follicle Stimulating Hormone
9002-68-0
ErbB Receptors
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1961-1971Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2019/14588-6
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 001
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
J Reprod Fertil. 1986 Mar;76(2):645-56
pubmed: 3084771
Anim Reprod Sci. 2004 Jul;82-83:431-46
pubmed: 15271471
J Assist Reprod Genet. 2019 May;36(5):905-913
pubmed: 30887159
Mol Reprod Dev. 2005 Jul;71(3):358-67
pubmed: 15822117
Biol Reprod. 2000 Feb;62(2):370-7
pubmed: 10642575
Mol Cell Endocrinol. 2010 May 14;320(1-2):87-96
pubmed: 20138115
Theriogenology. 2023 Jan 1;195:209-216
pubmed: 36368115
Front Immunol. 2018 Nov 29;9:2808
pubmed: 30555480
Nat Commun. 2021 Mar 4;12(1):1438
pubmed: 33664246
Endocrinology. 2005 Jan;146(1):77-84
pubmed: 15459120
Reproduction. 2011 Apr;141(4):425-35
pubmed: 21239527
Endocrinology. 2007 Dec;148(12):6164-75
pubmed: 17901238
Mol Cell Endocrinol. 2012 Jun 5;356(1-2):65-73
pubmed: 22101318
Mol Endocrinol. 2006 Apr;20(4):715-23
pubmed: 16051667
Anat Embryol (Berl). 1987;176(1):41-4
pubmed: 3605648
Mol Reprod Dev. 2007 Jun;74(6):740-9
pubmed: 17075796
Fertil Steril. 2011 Nov;96(5):1246-51.e1
pubmed: 21917251
Mol Reprod Dev. 2019 Nov;86(11):1639-1651
pubmed: 31389116
Curr Biol. 2018 Apr 23;28(8):R354-R356
pubmed: 29689210
Biol Reprod. 2004 Feb;70(2):465-72
pubmed: 14568913
Science. 2010 Oct 15;330(6002):366-9
pubmed: 20947764
Biol Reprod. 1997 Apr;56(4):976-84
pubmed: 9096881
Hum Reprod Update. 2021 Jan 4;27(1):27-47
pubmed: 33020823
Reproduction. 2013 Jun 14;146(1):27-35
pubmed: 23641036
Ital J Anat Embryol. 2001;106(2 Suppl 2):241-8
pubmed: 11732583
Mol Hum Reprod. 2009 Dec;15(12):779-88
pubmed: 19651638
Biol Reprod. 2018 Feb 1;98(2):162-169
pubmed: 29165545
J Reprod Dev. 2012;58(5):510-4
pubmed: 23124701
Endocrinology. 2003 Oct;144(10):4376-84
pubmed: 12959984
Endocrinology. 2018 Sep 1;159(9):3433-3445
pubmed: 30060157
Mol Reprod Dev. 2008 Jan;75(1):105-14
pubmed: 17549700
Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):E776-85
pubmed: 23382188
Reprod Fertil Dev. 2021 Dec;34(2):27-35
pubmed: 35231269
Biol Reprod. 2007 Oct;77(4):743-50
pubmed: 17582010
Hum Reprod. 2007 May;22(5):1247-52
pubmed: 17293344
Theriogenology. 2012 Sep 1;78(4):887-97
pubmed: 22626782
Reproduction. 2015 Jan;149(1):21-9
pubmed: 25323036
Fertil Steril. 2013 Aug;100(2):585-92.e1
pubmed: 23663993
Biol Reprod. 2005 Jan;72(1):195-9
pubmed: 15385414
J Biol Chem. 1989 Aug 15;264(23):13840-7
pubmed: 2503506
Hum Reprod Update. 2008 Mar-Apr;14(2):159-77
pubmed: 18175787
Dev Biol. 2015 Jul 15;403(2):139-49
pubmed: 25981108
Dev Biol. 1990 Aug;140(2):307-17
pubmed: 2115479
Development. 2008 Oct;135(19):3229-38
pubmed: 18776144
Proc Natl Acad Sci U S A. 2006 Jul 11;103(28):10678-83
pubmed: 16818886
Reprod Fertil Dev. 2017 Feb;29(2):234-243
pubmed: 26194863
Reprod Biomed Online. 2011 Apr;22(4):389-98
pubmed: 21353640
Science. 2004 Jan 30;303(5658):682-4
pubmed: 14726596
Reprod Fertil Dev. 2013;25(6):890-9
pubmed: 23021259
Science. 2009 May 15;324(5929):938-41
pubmed: 19443782
Hum Reprod Update. 2022 Feb 28;28(2):232-254
pubmed: 34969065
Biol Reprod. 2003 Jul;69(1):269-77
pubmed: 12620932
J Biol Chem. 2001 Apr 6;276(14):11387-92
pubmed: 11154695
Biol Reprod. 2002 Mar;66(3):707-17
pubmed: 11870078
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
Mol Endocrinol. 2014 May;28(5):706-21
pubmed: 24650175
Mol Endocrinol. 2006 Jun;20(6):1352-65
pubmed: 16543407
Reproduction. 2001 Dec;122(6):829-38
pubmed: 11732978
Hum Reprod. 2019 Nov 1;34(11):2244-2253
pubmed: 31725884
Theriogenology. 2000 Sep 15;54(5):741-56
pubmed: 11101035
Mol Endocrinol. 2011 Jan;25(1):104-16
pubmed: 21047912
Mol Reprod Dev. 2004 Nov;69(3):347-55
pubmed: 15349847
Dev Biol. 1990 Mar;138(1):16-25
pubmed: 2155145
Reprod Fertil Dev. 2009;21(5):655-64
pubmed: 19486602
Mol Endocrinol. 2010 Jun;24(6):1230-9
pubmed: 20382892
Hum Reprod Update. 2007 May-Jun;13(3):289-312
pubmed: 17242016
Reproduction. 2017 Dec;154(6):745-753
pubmed: 28874516
Endocrinology. 2012 Sep;153(9):4533-43
pubmed: 22778215
Reproduction. 2016 Nov;152(5):481-9
pubmed: 27492081
Biol Reprod. 2011 Dec;85(6):1252-9
pubmed: 21816847