SpkH (Sll0005) from Synechocystis sp. PCC 6803 is an active Mn
ABC1-type kinase
Casein kinase
Phosphorylation
Serine-threonine protein kinases
Synechocystis
Journal
Biochimie
ISSN: 1638-6183
Titre abrégé: Biochimie
Pays: France
ID NLM: 1264604
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
16
02
2023
revised:
23
04
2023
accepted:
10
05
2023
medline:
25
9
2023
pubmed:
21
5
2023
entrez:
20
5
2023
Statut:
ppublish
Résumé
Twelve genes for the potential serine-threonine protein kinases (STPKs) have been annotated in the genome of Synechocystis sp. PCC 6803. Based on similarities and distinctive domain organization, they were divided into two clusters: serine/threonine-protein N2-like kinases (PKN2-type) and "activity of bc1 complex" kinases (ABC1-type). While the activity of the PKN2-type kinases have been demonstrated, no ABC1-type kinases activity have hitherto been reported. In this study, a recombinant protein previously annotated as a potential STPK of ABC1-type (SpkH, Sll0005) was expressed and purified to homogeneity. We demonstrated SpkH phosphorylating activity and substrate preference for casein in in vitro assays using [γ-
Identifiants
pubmed: 37209809
pii: S0300-9084(23)00111-6
doi: 10.1016/j.biochi.2023.05.006
pii:
doi:
Substances chimiques
Protein Kinases
EC 2.7.-
Serine
452VLY9402
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114-122Informations de copyright
Copyright © 2023 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.