Progenitor-derived glia are required for spinal cord regeneration in zebrafish.
Glia
Neural repair
Regeneration
Spinal cord injury
Zebrafish
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
15 05 2023
15 05 2023
Historique:
received:
27
07
2022
accepted:
26
04
2023
pmc-release:
22
05
2024
medline:
24
5
2023
pubmed:
22
5
2023
entrez:
22
5
2023
Statut:
ppublish
Résumé
Unlike mammals, adult zebrafish undergo spontaneous recovery after major spinal cord injury. Whereas reactive gliosis presents a roadblock for mammalian spinal cord repair, glial cells in zebrafish elicit pro-regenerative bridging functions after injury. Here, we perform genetic lineage tracing, assessment of regulatory sequences and inducible cell ablation to define mechanisms that direct the molecular and cellular responses of glial cells after spinal cord injury in adult zebrafish. Using a newly generated CreERT2 transgenic line, we show that the cells directing expression of the bridging glial marker ctgfa give rise to regenerating glia after injury, with negligible contribution to either neuronal or oligodendrocyte lineages. A 1 kb sequence upstream of the ctgfa gene was sufficient to direct expression in early bridging glia after injury. Finally, ablation of ctgfa-expressing cells using a transgenic nitroreductase strategy impaired glial bridging and recovery of swim behavior after injury. This study identifies key regulatory features, cellular progeny, and requirements of glial cells during innate spinal cord regeneration.
Identifiants
pubmed: 37213080
pii: 310517
doi: 10.1242/dev.201162
pmc: PMC10233714
pii:
doi:
Substances chimiques
Zebrafish Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R01 NS113915
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS123708
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS096617
Pays : United States
Organisme : NINDS NIH HHS
ID : R21 NS124635
Pays : United States
Informations de copyright
© 2023. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests The authors declare no competing or financial interests.
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