Coinfection with porcine epidemic diarrhea virus and Clostridium perfringens type A enhances disease severity in weaned pigs.
Journal
Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870
Informations de publication
Date de publication:
22 May 2023
22 May 2023
Historique:
received:
30
01
2023
accepted:
25
04
2023
medline:
24
5
2023
pubmed:
23
5
2023
entrez:
22
5
2023
Statut:
epublish
Résumé
Clostridium perfringens is a constituent of the normal gut microbiome in pigs; however, it can potentially cause pre- and post-weaning diarrhea. Nevertheless, the importance of this bacterium as a primary pathogen of diarrhea in piglets needs to be better understood, and the epidemiology of C. perfringens in Korean pig populations is unknown. To study the prevalence and typing of C. perfringens, 203 fecal samples were collected from diarrheal piglets on 61 swine farms during 2021-2022 and examined for the presence of C. perfringens and enteric viruses, including porcine epidemic diarrhea virus (PEDV). We determined that the most frequently identified type of C. perfringens was C. perfringens type A (CPA; 64/203, 31.5%). Among the CPA infections, single infections with CPA (30/64, 46.9%) and coinfections with CPA and PEDV (29/64, 45.3%) were the most common in diarrheal samples. Furthermore, we conducted animal experiments to investigate the clinical outcome of single infections and coinfections with highly pathogenic (HP)-PEDV and CPA in weaned piglets. The pigs infected with HP-PEDV or CPA alone showed mild or no diarrhea, and none of them died. However, animals that were co-inoculated with HP-PEDV and CPA showed more-severe diarrheal signs than those of the singly infected pigs. Additionally, CPA promoted PEDV replication in coinfected piglets, with high viral titers in the feces. A histopathological examination revealed more-severe villous atrophy in the small intestine of coinfected pigs than in singly infected pigs. This indicates a synergistic effect of PEDV and CPA coinfection on clinical disease in weaned piglets.
Identifiants
pubmed: 37217624
doi: 10.1007/s00705-023-05798-3
pii: 10.1007/s00705-023-05798-3
pmc: PMC10202354
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
166Subventions
Organisme : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry
ID : 321018-1
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
Références
Chan G, Farzan A, Soltes G, Nicholson VM, Pei Y, Friendship R, Prescott JF (2012) The epidemiology of Clostridium perfringens type A on Ontario swine farms, with special reference to cpb2-positive isolates. BMC Vet Res 8:156
doi: 10.1186/1746-6148-8-156
pubmed: 22947389
pmcid: 3503845
Baums CG, Schotte U, Amtsberg G, Goethe R (2004) Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates. Vet Microbiol 100:11–16
doi: 10.1016/S0378-1135(03)00126-3
pubmed: 15135508
Mertens N, Theuß T, Köchling M, Dohmann K, Lillie-Jaschniski K (2022) Pathogens detected in 205 German farms with porcine neonatal diarrhea in 2017. Vet Sci 9:44
doi: 10.3390/vetsci9020044
pubmed: 35202297
pmcid: 8879730
Mesonero-Escuredo S, Strutzberg-Minder K, Casanovas C, Segalés J (2018) Viral and bacterial investigations on the aetiology of recurrent pig neonatal diarrhoea cases in Spain. Porcine Health Manag 4:5
doi: 10.1186/s40813-018-0083-8
pubmed: 29632701
pmcid: 5885353
Schoch CL, Ciufo S, Domrachev M, Hotton CL, Kannan S, Khovanskaya R, Leipe D, Mcveigh R, O'Neill K, Robbertse B, Sharma S, Soussov V, Sullivan JP, Sun L, Turner S, Karsch-Mizrachi I (2020) NCBI Taxonomy: a comprehensive update on curation, resources and tools. Database 2020:baaa062
doi: 10.1093/database/baaa062
pubmed: 32761142
pmcid: 7408187
Lee C (2015) Porcine epidemic diarrhea virus: an emerging and re-emerging epizootic swine virus. Virol J 12:193
doi: 10.1186/s12985-015-0421-2
pubmed: 26689811
pmcid: 4687282
Lee C (2019) Porcine epidemic diarrhoea virus. In: Zakaryan H (ed) Porcine viruses: from pathogenesis to strategies for control. Caister Academic Press, Norfolk, UK, pp 107–134
Choudhury B, Dastjerdi A, Doyle N, Frossard JP, Steinbach F (2016) From the field to the lab - An European view on the global spread of PEDV. Virus Res 226:40–49
doi: 10.1016/j.virusres.2016.09.003
pubmed: 27637348
Curry SM, Schwartz KJ, Yoon KJ, Gabler NK, Burrough ER (2017) Effects of porcine epidemic diarrhea virus infection on nursery pig intestinal function and barrier integrity. Vet Microbiol 211:58–66
doi: 10.1016/j.vetmic.2017.09.021
pubmed: 29102122
Jung K, Saif LJ (2015) Porcine epidemic diarrhea virus infection: Etiology, epidemiology, pathogenesis and immunoprophylaxis. Vet J 204:134–143
doi: 10.1016/j.tvjl.2015.02.017
pubmed: 25841898
pmcid: 7110711
Lee S, Kim Y, Lee C (2015) Isolation and characterization of a Korean porcine epidemic diarrhea virus strain KNU-141112. Virus Res 208:215–224
doi: 10.1016/j.virusres.2015.07.010
pubmed: 26196056
Lee S, Lee C (2018) Genomic and antigenic characterization of porcine epidemic diarrhoea virus strains isolated from South Korea, 2017. Transbound Emerg Dis 65:949–956
doi: 10.1111/tbed.12904
pubmed: 29770590
pmcid: 7169816
Lee S, Lee DU, Noh YH, Lee SC, Choi HW, Yang HS, Seol JH, Mun SH, Kang WM, Yoo H, Lee C (2019) Molecular characteristics and pathogenic assessment of porcine epidemic diarrhoea virus isolates from the 2018 endemic outbreaks on Jeju Island, South Korea. Transbound Emerg Dis 66:1894–1909
doi: 10.1111/tbed.13219
pubmed: 31055885
pmcid: 7168548
Jang G, Lee KK, Kim SH, Lee C (2017) Prevalence, complete genome sequencing and phylogenetic analysis of porcine deltacoronavirus in South Korea, 2014–2016. Transbound Emerg Dis 64:1364–1370
doi: 10.1111/tbed.12690
pubmed: 28758347
pmcid: 7169712
Jang G, Park J, Lee C (2021) Successful eradication of porcine epidemic diarrhea in an enzootically infected farm: a two-year follow-up study. Pathogens 10:830
doi: 10.3390/pathogens10070830
pubmed: 34357980
pmcid: 8308665
Park J, Lee C (2020) Emergence and evolution of novel G2b-like porcine epidemic diarrhea virus inter-subgroup G1b recombinants. Arch Virol 165:2471–2478
doi: 10.1007/s00705-020-04767-4
pubmed: 32772174
pmcid: 7414897
Lee S, Son KY, Noh YH, Lee SC, Choi HW, Yoon IJ, Lee C (2017) Genetic characteristics, pathogenicity, and immunogenicity associated with cell adaptation of a virulent genotype 2b porcine epidemic diarrhea virus. Vet Microbiol 207:248–258
doi: 10.1016/j.vetmic.2017.06.019
pubmed: 28757031
pmcid: 7172462
Jang G, Won H, Lee DU, Noh YH, Lee SC, Choi HW, Yoon IJ, Lee YJ, Sang Yoo H, Lee C (2019) Assessment of the safety and efficacy of an attenuated live vaccine based on highly virulent genotype 2b porcine epidemic diarrhea virus in nursing piglets. Vet Microbiol 231:120–128
doi: 10.1016/j.vetmic.2019.03.009
pubmed: 30955799
pmcid: 7117296
Wang X, Ren W, Nie Y, Cheng L, Tan W, Wang C, Wei L, Zhang R, Yan G (2013) A novel watery diarrhoea caused by the co-infection of neonatal piglets with Clostridium perfringens type A and Escherichia coli (K88, 987P). Vet J 197:812–816
doi: 10.1016/j.tvjl.2013.05.023
pubmed: 23846029
Baek PS, Choi HW, Lee S, Yoon IJ, Lee YJ, Lee DS, Lee S, Lee C (2016) Efficacy of an inactivated genotype 2b porcine epidemic diarrhea virus vaccine in neonatal piglets. Vet Immunol Immunopathol 174:45–49
doi: 10.1016/j.vetimm.2016.04.009
pubmed: 27185262
pmcid: 7126956
Wang B, Russell ML, Fonseca K, Earn DJD, Horsman G, Van Caeseele P, Chokani K, Vooght M, Babiuk L, Walter SD, Loeb M (2017) Predictors of influenza a molecular viral shedding in Hutterite communities. Influenza Other Respir Viruses 11:254–262
doi: 10.1111/irv.12448
pubmed: 28207989
pmcid: 5410723
Jung K, Kim J, Ha Y, Choi C, Chae C (2006) The effects of transplacental porcine circovirus type 2 infection on porcine epidemic diarrhoea virus-induced enteritis in preweaning piglets. Vet J 171:445–450
doi: 10.1016/j.tvjl.2005.02.016
pubmed: 16624710
Dors A, Czyżewska-Dors E, Wasyl D, Pomorska-Mól M (2016) Prevalence and factors associated with the occurrence of bacterial enteropathogens in suckling piglets in farrow-to-finish herds. Vet Rec 179:598
doi: 10.1136/vr.103811
pubmed: 27655436
Vidal A, Martín-Valls GE, Tello M, Mateu E, Martín M, Darwich L (2019) Prevalence of enteric pathogens in diarrheic and non-diarrheic samples from pig farms with neonatal diarrhea in the North East of Spain. Vet Microbiol 237:108419
doi: 10.1016/j.vetmic.2019.108419
pubmed: 31585655
pmcid: 7117353
Gould LH, Limbago B (2010) Clostridium difficile in food and domestic animals: a new foodborne pathogen? Clin Infect Dis 51:577–582
doi: 10.1086/655692
pubmed: 20642351
Springer S, Finzel J, Florian V, Schoepe H, Woitow G, Selbitz HJ (2012) Occurrence and control of the Clostridium perfringens type A associated diarrhea of the suckling pigs with special consideration of the immunoprophylaxis. Tierarztl Prax Ausg G Grosstiere Nutztiere 40:375–382
pubmed: 23242147
Johannsen U, Arnold P, Köhler B, Selbitz HJ (1993) Untersuchungen zur experimentellen Clostridium perfringens-Typ-AEnterotoxämie der Saugferkel. MH Vet Med 48:129–136