Leptospiral imelysin (LIC_10713) is secretory, immunogenic and binds to laminin, fibronectin, and collagen IV.
Diagnosis
Imelysin
Invasin
LIC10713
Leptospira
Leptospirosis
Peptidase M75
Putative lipoprotein
Secretory
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
25
01
2023
accepted:
09
05
2023
revised:
03
05
2023
medline:
3
7
2023
pubmed:
25
5
2023
entrez:
25
5
2023
Statut:
ppublish
Résumé
Leptospirosis is a widespread zoonotic disease caused by pathogenic Leptospira. Early and accurate diagnosis is the prime step in managing the disease. Secretory proteins of Leptospira remain distinguished for diagnosis due to their availability as soluble proteins in the serum and their interaction with the host immune response due to their extracellular presence. This study presents the cloning, expression, purification, and characterization of imelysin or LruB (LIC_10713), a putative leptospiral protein. We report that the localization of imelysin showed its presence in the inner membrane and in the culture supernatant. The imelysin was upregulated under in vitro physiological conditions of infection. The LIC_10713 interacted significantly with laminin, fibronectin, collagen type I, and collagen type IV in a dose-dependent manner. Phylogenetic analysis showed that LIC_10713 is predominately found in the pathogenic species of Leptospira, and the GxHxxE motif of imelysin-like proteins is represented as the amino acid sequence G
Identifiants
pubmed: 37227474
doi: 10.1007/s00253-023-12573-6
pii: 10.1007/s00253-023-12573-6
doi:
Substances chimiques
Laminin
0
Fibronectins
0
Collagen Type IV
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4275-4289Subventions
Organisme : Indian Council of Medical Research, New Delhi, India.
ID : Leptos/22/2013-ECD-I-2012-2400
Organisme : Indian Council of Medical Research, New Delhi, India.
ID : ICMR-SRF 2020-0756/PROTEOMICS-BMS
Organisme : Indian Council of Medical Research, New Delhi, India.
ID : Grant No: Fellowship/131/2022-ECD-II-2021-8230
Organisme : Department of Science and Technology, Science and Engineering Research Board, New Delhi, India
ID : SR/SO/HS/0108/2012
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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