Microglia mediate neurocognitive deficits by eliminating C1q-tagged synapses in sepsis-associated encephalopathy.


Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
26 05 2023
Historique:
medline: 29 5 2023
pubmed: 26 5 2023
entrez: 26 5 2023
Statut: ppublish

Résumé

Sepsis-associated encephalopathy (SAE) is a severe and frequent complication of sepsis causing delirium, coma, and long-term cognitive dysfunction. We identified microglia and C1q complement activation in hippocampal autopsy tissue of patients with sepsis and increased C1q-mediated synaptic pruning in a murine polymicrobial sepsis model. Unbiased transcriptomics of hippocampal tissue and isolated microglia derived from septic mice revealed an involvement of the innate immune system, complement activation, and up-regulation of lysosomal pathways during SAE in parallel to neuronal and synaptic damage. Microglial engulfment of C1q-tagged synapses could be prevented by stereotactic intrahippocampal injection of a specific C1q-blocking antibody. Pharmacologically targeting microglia by PLX5622, a CSF1-R inhibitor, reduced C1q levels and the number of C1q-tagged synapses, protected from neuronal damage and synapse loss, and improved neurocognitive outcome. Thus, we identified complement-dependent synaptic pruning by microglia as a crucial pathomechanism for the development of neuronal defects during SAE.

Identifiants

pubmed: 37235660
doi: 10.1126/sciadv.abq7806
pmc: PMC10219600
doi:

Substances chimiques

Complement C1q 80295-33-6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

eabq7806

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Auteurs

Ha-Yeun Chung (HY)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.
Center for Sepsis Control and Care, Jena University Hospital, Jena 07747, Germany.

Jonathan Wickel (J)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.
Center for Sepsis Control and Care, Jena University Hospital, Jena 07747, Germany.

Nina Hahn (N)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.
Center for Sepsis Control and Care, Jena University Hospital, Jena 07747, Germany.

Nils Mein (N)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.

Meike Schwarzbrunn (M)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.

Philipp Koch (P)

Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Jena 07745, Germany.

Mihai Ceanga (M)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.

Holger Haselmann (H)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.

Carolin Baade-Büttner (C)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.

Nikolai von Stackelberg (N)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.

Nina Hempel (N)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.

Lars Schmidl (L)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.

Marco Groth (M)

Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Jena 07745, Germany.

Nico Andreas (N)

Institute of Immunology, Jena University Hospital, Jena 07743, Germany.

Juliane Götze (J)

Department of Anaesthesiology and Intensive Care Medicine, Jena University Hospital, Jena 07747, Germany.
Septomics Research Center, Jena University Hospital, Jena 07745, Germany.

Sina M Coldewey (SM)

Center for Sepsis Control and Care, Jena University Hospital, Jena 07747, Germany.
Department of Anaesthesiology and Intensive Care Medicine, Jena University Hospital, Jena 07747, Germany.
Septomics Research Center, Jena University Hospital, Jena 07745, Germany.

Michael Bauer (M)

Center for Sepsis Control and Care, Jena University Hospital, Jena 07747, Germany.
Department of Anaesthesiology and Intensive Care Medicine, Jena University Hospital, Jena 07747, Germany.

Christian Mawrin (C)

Department of Neuropathology, University of Magdeburg, Magdeburg, Germany.
Section of Pathology, Institute of Forensic Medicine, Jena University Hospital, Jena 07749, Germany.

Justina Dargvainiene (J)

Neuroimmunology, Institute of Clinical Chemistry, UKSH, Kiel/Lübeck, Germany.

Frank Leypoldt (F)

Neuroimmunology, Institute of Clinical Chemistry, UKSH, Kiel/Lübeck, Germany.
Department of Neurology, Christian-Albrechts University, Kiel 24105, Germany.

Stephan Steinke (S)

Department Medical Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Braunschweig, Germany.

Zhao-Qi Wang (ZQ)

Leibniz Institute on Aging - Fritz Lipmann Institute (FLI), Jena 07745, Germany.
Faculty of Biological Sciences, Friedrich Schiller University, Jena 07745, Germany.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, China.

Michael Hust (M)

Department Medical Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics, Technische Universität Braunschweig, Braunschweig, Germany.

Christian Geis (C)

Section of Translational Neuroimmunology, Department of Neurology, Jena University Hospital, Jena 07747, Germany.
Center for Sepsis Control and Care, Jena University Hospital, Jena 07747, Germany.

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