Airway proteolytic control of pneumococcal competence.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
05 2023
05 2023
Historique:
received:
07
07
2022
accepted:
11
05
2023
revised:
12
06
2023
medline:
14
6
2023
pubmed:
31
5
2023
entrez:
31
5
2023
Statut:
epublish
Résumé
Streptococcus pneumoniae is an opportunistic pathogen that colonizes the upper respiratory tract asymptomatically and, upon invasion, can lead to severe diseases including otitis media, sinusitis, meningitis, bacteremia, and pneumonia. One of the first lines of defense against pneumococcal invasive disease is inflammation, including the recruitment of neutrophils to the site of infection. The invasive pneumococcus can be cleared through the action of serine proteases generated by neutrophils. It is less clear how serine proteases impact non-invasive pneumococcal colonization, which is the key first step to invasion and transmission. One significant aspect of pneumococcal biology and adaptation in the respiratory tract is its natural competence, which is triggered by a small peptide CSP. In this study, we investigate if serine proteases are capable of degrading CSP and the impact this has on pneumococcal competence. We found that CSP has several potential sites for trypsin-like serine protease degradation and that there were preferential cleavage sites recognized by the proteases. Digestion of CSP with two different trypsin-like serine proteases dramatically reduced competence in a dose-dependent manner. Incubation of CSP with mouse lung homogenate also reduced recombination frequency of the pneumococcus. These ex vivo experiments suggested that serine proteases in the lower respiratory tract reduce pneumococcal competence. This was subsequently confirmed measuring in vivo recombination frequencies after induction of protease production via poly (I:C) stimulation and via co-infection with influenza A virus, which dramatically lowered recombination events. These data shed light on a new mechanism by which the host can modulate pneumococcal behavior and genetic exchange via direct degradation of the competence signaling peptide.
Identifiants
pubmed: 37256908
doi: 10.1371/journal.ppat.1011421
pii: PPATHOGENS-D-22-01194
pmc: PMC10259803
doi:
Substances chimiques
Serine Proteases
EC 3.4.-
Peptides
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1011421Subventions
Organisme : NIAID NIH HHS
ID : U01 AI124302
Pays : United States
Organisme : NIAID NIH HHS
ID : R56 AI155614
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI168214
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI171038
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI158076
Pays : United States
Informations de copyright
Copyright: © 2023 Echlin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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