Photoelectrochemistry of a photosystem I - Ferredoxin construct on ITO electrodes.
Biosynthesis
Ferredoxin NADP(+) reductase
ITO electrode
Photobioelectrochemistry
Photosystem I
Journal
Bioelectrochemistry (Amsterdam, Netherlands)
ISSN: 1878-562X
Titre abrégé: Bioelectrochemistry
Pays: Netherlands
ID NLM: 100953583
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
06
01
2023
revised:
26
04
2023
accepted:
03
05
2023
medline:
23
10
2023
pubmed:
2
6
2023
entrez:
1
6
2023
Statut:
ppublish
Résumé
In this study, photobioelectrodes based on a ferredoxin-modified photosystem I (PSI-Fd) from Thermosynechococcus vestitus have been prepared and characterized regarding the direct electron transfer between PSI-Fd and the electrode. The modified PSI with the covalently linked ferredoxin (Fd) on its stromal side has been immobilized on indium-tin-oxide (ITO) electrodes with a 3-dimensional inverse-opal structure. Compared to native PSI, a lower photocurrent and a lower onset potential of the cathodic photocurrent have been observed. This can be mainly attributed to a different adsorption behavior of the PSI-Fd-construct onto the 3D ITO. However, the overall behavior is rather similar to PSI. First experiments have been performed for applying this PSI-Fd photobioelectrode for enzyme-driven NADPH generation. By coupling the electrode system with ferredoxin-NADP
Identifiants
pubmed: 37263168
pii: S1567-5394(23)00096-8
doi: 10.1016/j.bioelechem.2023.108459
pii:
doi:
Substances chimiques
Photosystem I Protein Complex
0
Ferredoxins
0
NADP
53-59-8
Ferredoxin-NADP Reductase
EC 1.18.1.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108459Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.